31 research outputs found

    海洋储碳机制及区域碳氮硫循环耦合对全球变化的响应

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    海洋作为地球表面最大的活跃碳库,其碳收支在很大程度上决定了全球气候变化的走向.然而,海洋碳循环是一个多时空尺度的过程,相关的碳收支估算存在很大的; 不确定性,其控制过程与机理更是一个颇具挑战性的难题(胡敦欣等, 2015),特别是海洋储碳机制,是研究全球变化及应对的核心内容之一.国家重点研发计划专项全球变化及应对项

    Proteomic Analysis of Rice Cultivar Jiafuzhan in the Responses to Xanthomonas campestris pv.oryzicola Infection

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    作者简介: 陈芳育(1978-) , 男, 讲师。E-mail : cfy307@ sohu. com * 通讯作者(Corresponding author) : 陈亮( 1963-) , 男, 教授, 博士生导师, 研究方向: 细胞与分子生物学。E-mail: chenlg@ xmu. edu. cn[中文文摘]运用双向电泳分析高抗水稻品种“佳辐占”受强毒力细菌性条斑病病原菌侵染2d后的叶片蛋白质组变化,共发现38个蛋白质发生差异表达,其中32个上调,5个下调,1个新增。用MALDI-TOF-MS分析和数据库检索鉴定出其中的33个差异表达蛋白质,并将它们分为4个功能类群,即信号转导相关蛋白、防卫相关蛋白、代谢相关蛋白和蛋白质稳定相关蛋白。这些蛋白分别参与了信号识别、信号传递、抗氧化、糖代谢、细胞壁加固、植保素合成等抗病生理反应。研究表明,水稻对细菌性条斑病病原菌的侵染存在着一个复杂的抗病信号应答和代谢调控网络,其作用机理可以通过差异表达的蛋白质(酶)反映出来,其中差异表达的8个R蛋白和3个PR蛋白可能与水稻对细菌性条斑病的抗病性密切相关。本研究为进一步揭示水稻对细菌性条斑病的抗性机理及相关抗病基因的功能克隆提供了依据。[英文文摘]Rice bacterial leaf streak( BLS) caused by the pathogen Xanthomonas oryzae pv. oryzicola ( Xooc) is one of the major rice diseases in South China. Here we focus on proteomics as a tool for the discovery of differentially expressed proteins closely related to the disease resistance. The leaves of rice cultivar Jiafuzhan (Oryzae sativa L. ) highly resistant to the disease, were infected by"89773-1- 1" strain of the Xooc with strong pathogenicity. Total proteins were extracted from the leaves sampled at two days after inoculation, and separated by two- dimensional electrophoresis. It was found that there were thirty- eight proteins expressed differentially, of which thirty-two were up-regulated, five down-regulated and one was "new". Of the thirty- eight responsive proteins, thirty-three were identified by MALD-I TOF-MS and database searching.Based on the predicted function, we grouped them into four clusters: signal transduction, defensive responses, substance metabolism and protein stabilization, which were involved in many resistant physiological react ions, including signal recognition and transduction, antioxidant react ion, carbonhydrate metabolism, cel-l wall reinforcement and phytoalexin biosythesis. In turn a complex signal transduct ion and metabolic regulative network in the resistant responses to the infection of Xooc was outlined in this work, and the molecular mechanism was revealed by differentially expressed protein/enzyme patterns during Xooc infection. In this study, eight R proteins and three pathogenesis- related(PR) proteins which might relate closely to the disease-resistance were found. This result provides us the basic information to further reveal the resistant mechanism and conduct functional cloning of the resistan-t related genes in rice to BLS.生物农药与化学生物学教育部重点实验室( 福建农林大学) 开放课题基金项目( KF0411

    Honeycomb-Patterned Hybrid Films and Their Template Applications via A Tunable Amphiphilic Block Polymer/Inorganic Precursor System

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    通讯作者地址: Li,L(通讯作者),Xiamen Univ,Coll Mat, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Mat, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 3. Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 电子邮件地址: [email protected], we show a facile and versatile method-prepare highly ordered inorganic patterns on solid substrates by pyrolyzing UV cross-linked polymr/functional. precursor hybrid films. The crosslinked polymer matrix acted as structure-directing agent in a pyrolyzing process, whereas the functional precursor was converted into the skeleton of the micropatterns. The inorganic micropatterns could be further catalytically functionalized to grow CNT and ZnO nanorod arrays by simply changing different functional precursors. This simple technique offers new prospects in the field of micropatterns, nanolithography, and template.National Natural Science Foundation of China,50703032,20974089;Natural Science Foundation of Fujian Province,2009J06029; Ministry of Education of Chin

    Patterned Carbon Nanotubes with Adjustable Array: A Functional Breath Figure Approach

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    通讯作者地址: Xie, SY (通讯作者), Xiamen Univ, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Mat, Xiamen 361005, Peoples R China 3. Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 电子邮件地址: [email protected], [email protected] is of paramount importance in many areas of modern science and technology. As a valuable part in miniaturized devices, large-scale aligned CNTs with serial port configuration is highly desirable. Here, we reported that the synthesis of a pair of patterned carbon nanotubes with the shape of serial port by CVD starting from different breath figure templates, cross-linked polymer matrix and ferrous inorganic micropatterns. The growth mechanism of the isolated CNT bundles is attributed to the selectively interfacial aggregation of the ferrocene to the walls of the cavities, a so-called Pickering-emulsion effect, whereas the honeycomblike skeleton of the dense CNT arrays develop from the catalytically functionalized hexagonal edges. This synthesis strategy exemplifies a new possibility for making use of CNTs to fabricate functional carbons with unique geometry or specific properties in a controllable way. We expect that the methodology can be also applied onto the fabrication of patterned graphene.National Natural Science Foundation of China 50703032,20974089 Natural Science Foundation of Fujian Province 2009J06029 Ministry of Education of Chin

    最近130 ka以来黄土中Zr/Rb值变化及其对冬季风的指示意义

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    Advances in the research of methane oxidation in forest soils

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    甲烷是一种重要的温室气体,对全球气候变暖的贡献仅次于CO2,约为25%。大气甲烷可以被土壤中甲烷氧化细菌在有氧条件下吸收利用,陆地生态系统森林土壤氧化吸收甲烷的研究已有大量报道。甲烷氧化菌是以甲烷作为唯一的碳源和能源的一类细菌的总称。但森林土壤在全球甲烷核算中具有一定的不确定性,取决于产甲烷菌和甲烷氧化菌的相对活性。甲烷氧化菌的研究集中在环境对氧化能力的影响和自身氧化能力上。大气甲烷氧化过程为高氧化能力低亲力氧化,受到一些因子,如土壤温度、土壤通气状况、pH、氮肥等的影响,具体机理的研究还有待进一步深入。土壤通气状况受土壤质地与土壤水分影响,土地利用类型可能改变土壤容重、土壤结构和土壤水分,进而影响土壤甲烷氧化。植物可以通过自身对生境的作用或化感作用影响土壤甲烷氧化。土壤动物的研究则相对较少,目前排放清单中仅有白蚁是全球甲烷核算所包括的。从甲烷氧化菌的分类出发,对甲烷氧化菌氧化甲烷的机理、菌的生态分布及甲烷氧化的影响因素、时空异质性、观测方法等作出了综述,为正确认识和准确预测森林土壤在一定气候和土地利用类型条件下的甲烷氧化强度提供理论依据

    藻-菌生态系统代谢功能的生态学研究

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    在室内模拟条件下,研究了一些生态因子对藻-菌(A+B)生态系统代谢有机碳(C6H12O6)、NH3-N和无机磷(IP)的影响.研究结果表明,当藻-菌生态系统中藻(A)或菌(B)的起始数量一定时,其代谢C6H12O6的速率,随与之组合的B或A的起始数量增加(数量比则相应降低)而增加.在光照和黑暗条件下,A+B系统代谢上述3种营养物质的速率均有一定的差异.黑暗下C6H12O6的平均代谢速率较光照下高12.3%(P<0.05),IP和NH3-N的平均代谢速率则分别较光照下低14.4%(P<0.05)和16.2
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