石油和煤微生物脱硫技术的研究进展

生物脱硫技术在能源工业发展和环境保护等方面显示出潜在的优势. 本文综述了应用于化石燃料生物脱硫的主要菌种及其脱硫原理、微生物的选育优化及微生物脱硫的动力学, 介绍了生物脱硫技术的工业化应用. 对生物脱硫技术的经济性进行了分析, 指出了进一步研究微生物脱硫技术存在的问题和在我国发展该技术的重要性

微生物脱硫过程中的产物分析

微生物脱除矿物燃料中的杂环有机硫分为微生物降解脱硫和微生物催化脱硫，前一途径中菌株通过断裂有机化合物的C-C键，降解模型化合物二苯并噻吩（DBT）生成含硫水溶性产物从燃料中脱除，破坏了碳氢化合物的碳骨架，引起燃烧值的降低。后一途径中微生物或它们的酶催化氧化DBT的杂环硫原子，生成二苯并噻吩砜（DBTO_2）及终产物2-羟基联苯（HBP）和硫酸盐（‘4S’途径），保留了整个碳氢化合物的骨架。本研究用DBT作为唯一硫源筛选不同的菌种。通过液相色谱（HPLC）和液相色谱/质谱/质谱联用（LC-MS-MS）法定性、定量分析代谢物，从而了解所筛菌种的脱硫途径及脱硫效果

Application of a Lanthanide Fluorescence System Coupled with the Oxidation Reaction of p-HBA Catalyzed by FeTSPc to the Determination of H_2O_2

[中文文摘]用四磺基酞菁铁(FeTSPc)催化H2O2氧化对羟基苯甲酸(p HBA)形成弱荧光的二聚产物———联二对 羟基苯甲酸,该产物在碱性条件下能与铽、EDTA形成三元配合物,产生较强的分子内敏化发光,表现出铽离子的特征荧光发射.据此,建立了雨水中过氧化氢和血清中葡萄糖的灵敏测定方法.在最佳条件下,过氧化氢的线性范围为0～2.0×10-5mol/L,检测限为2.5×10-8mol/L.[英文文摘]The oxidation product of phydroxybenzoic acid (pHBA) oxidated by hydrogen peroxide in the presence of iron(III)tetrasulfonatophthalocyanine (FeTSPc) forms a ternary fluorescent complex with Tb3+ in the alkaline medium. Based on this fact, a novel lanthanide fluorescence system with a coupling reaction was developed for the determination of hydrogen peroxide in rainwater and glucose in human serum. Under optimum conditions, the calibration graph was linear over the range of 0～2.0× 10 -5 mol/L.The detection limit of this method was 2.5 ×10- 8 mol/ L for hydrogen peroxide , and the standard deviation ( n = 9) was 3. 13 %for the determination of 1.0 ×10- 6 mol/ L hydrogen peroxide. This method can be applied to the determination of biochemical substances which produce hydrogen peroxide in oxidation reactions catalyzed by their oxidases. The feasibility in practical clinical analysis has been proven by the determination of glucose in human serum.福建省青年科技人才创新项目(2001J042)资

微生物脱硫过程中的产物分析

微生物脱除矿物燃料中的杂环有机硫分为微生物降解脱硫和微生物催化脱硫，前一途径中菌株通过断裂有机化合物的C-C键，降解模型化合物二苯并噻吩（DBT）生成含硫水溶性产物从燃料中脱除，破坏了碳氢化合物的碳骨架，引起燃烧值的降低。后一途径中微生物或它们的酶催化氧化DBT的杂环硫原子，生成二苯并噻吩砜（DBTO_2）及终产物2-羟基联苯（HBP）和硫酸盐（‘4S’途径），保留了整个碳氢化合物的骨架。本研究用DBT作为唯一硫源筛选不同的菌种。通过液相色谱（HPLC）和液相色谱/质谱/质谱联用（LC-MS-MS）法定性、定量分析代谢物，从而了解所筛菌种的脱硫途径及脱硫效果

石油生物催化深度脱硫

简要综述了国外石油生物催化脱硫研究的新进展,介绍了石油生物催化脱硫的发展趋势和应用前景

A Novel Method for the Determination of Nucleic Acids Based on Enzyme Catalyzed Fluorescence Reaction

A novel fluorimetric method has been developed for the rapid determination of ncleic acids, based on that the catalytic activity of hemin is decreased due to the ion-association with Alcian blue 8GX and recovered when nucleic acids are added to the system. The change in the catalytic activity of hemin results in the fluorescence change of the hemin-catalyzed fluorogenic reaction system of p-hydroxyphenylacetic acid(p-HPA) and H2O2 and the fluorescence change of the system is proportional to the quantities of nucleic acids existing. The corresponding detect ion lim it s of the method are 0.91 ng/mL for CT DNA and 7.5 ng/mL for yeast RNA

Determination of Nucleic Acids Based on Shifting the Association Equilibrium between Tetrasulphonated Aluminum Phthalocyanine and Alcian Blue

[中文文摘]阴离子荧光染料四磺基铝酞菁与阳离子荧光染料爱尔新蓝的缔合作用 ,使四磺基铝酞菁发生荧光猝灭 ,而当核酸存在时 ,染料缔合平衡受到影响而导致四磺基铝酞菁的荧光恢复。根据这一原理 ,建立了核酸定量测定的新方法。方法具有很高的灵敏度和较好的选择性 ,其线性范围为 0～ 2 0 0 μg/L ;检测限分别为 1.8μg/L(SMDNA)、2 .0 μg/L(CTDNA)、5 .4μg/L(酵母RNA)。将方法用于实际样品金黄色葡萄球菌中DNA含量的测定 ,获得满意结果。[英文文摘]Based on the ability of nucleic acids to shift the association equilibrium of an ion association complex of alcian blue and tetra sulfonated aluminum phthalocyanine, thus leading to an increase in phthalocyanine fluorescence, a new method was suggested for the fluorimetric determination of nucleic acids. Investigations were carried out on the spectral characteristic, selection of the buffer system, effect of pH, influence of reaction time, the usage of reagents and interference of foreign substances.Under optimum conditions , the calibration curves for determinations of calf thymus deoxyribonucleic (DNA) (CTDNA) , salmon DNA (SMDNA) and yeast ribonucleic acid (RNA) were linear over the range of 2.0～200μg/ L. The detection limits for CTDNA , SMDNA and RNA were 2.0 μg/ L , 1.8μg/ L and 5.4μg/ L , respectively. This method was used to the determination of golden staplylococuus DNA and the result was in agreement with that achieved by an ultraviolet spectrophotometric method

一种自动变位电动拧紧装置

本发明涉及一种螺栓拧紧装置，具体地说是一种通过位置平移和角度旋转使电动拧紧机自动对准螺栓的一种自动变位电动拧紧装置，包括升降机构、平移机构、推动旋转机构和电动拧紧机，所述平移机构安装在所述升降机构上，所述平移机构通过所述升降机构带动升降，所述电动拧紧机和推动旋转机构均安装在所述平移机构上，所述电动拧紧机和推动旋转机构通过所述平移机构带动平移，在所述平移机构上设有旋转座，电动拧紧机安装在所述旋转座上，所述旋转座通过所述推动旋转机构驱动旋转。本发明拧紧效率高且拧紧质量好，能够保证拧紧装配的高节拍和高质量的要求

表面活性剂对微生物脱除柴油中有机硫的影响

采用假单胞菌(Pseudomonas delafieldii)菌株R-8和红色红球菌(Rhodococcus erythropolis)菌株N1-36研究了加氢精制柴油脱硫工艺,两株菌脱除柴油中有机硫的活性相近. 添加表面活性剂能提高菌株对柴油的脱硫率;当Tween80存在、搅拌转速为250 r/min时,菌株R-8最高可脱除硫含量<300 mg/L的柴油中72%的有机硫;但当硫含量超过1000 mg/L时,微生物脱硫率极低