Study on the thermotolerance mechanisms of Agaricus bisporus as well as the cloning and functional verification of related genes

高温胁迫(Heatstress，HS)指生物体处于超过其适宜温度范围的环境下所受到的一种非生物胁迫(Abioticstress)，它能破坏生物体正常的细胞动态平衡，导致生物体严重的生长、发育停滞，甚至死亡。随着温室效应的影响和全球变暖的趋势，高温胁迫以及与之紧密关联的干旱、渗透等胁迫已经成为导致全球性农作物减产的主要原因。本文主要研究高温胁迫对双孢蘑菇的损害，以及耐温双孢蘑菇菌株02对高温的响应和抵御机制；同时探讨了应用分子生物学和基因工程技术培育耐高温双孢蘑菇品种的可能性。 双孢蘑菇[AgaricusbisporusLange(Imbach)]是具有高经济价值和生态意义的栽培食用菌。双孢蘑...Heat stress is one type of abiotic stress, which caused by high environmental temperature beyond optimum. Heat stress could disturb the cellular homeostasis of organisms, leading to sever stagnation in growth, development, and even death. As the global climate getting warmer, heat stress becomes the main cause for the great reduction in agriculture and breeding. In this dissertation, we investigat...学位：理学博士院系专业：生命科学学院生物化学与生物技术系_微生物学学号：2162006015330

Research on the Dimerization Sequence of Exo70 and Its Role in Breast Cancer Cell Metastasis

Exo70是胞外分泌复合体Exocyst的关键亚基,可通过参与Exocyst复合体或自身聚合促进细胞迁移。我们在研究中发现,Exo70自身形成二聚体的能力显著强于与Exocyst其它7个亚基的结合,提示Exo70二聚化的重要作用。进一步构建Exo70缺失突变体,并通过一系列免疫共沉淀实验确定了Exo70形成二聚体的关键位置是位于氨基酸31~35与505~509的两段同样的序列SLEKS。进而利用这两个序列的缺失突变体,通过transwell迁移实验,证实这两段SLEKS序列在Exo70二聚化及乳腺癌细胞迁移过程中的关键作用。以上结果为Exo70独立于Exocyst复合体功能之外的新功能研究提供了依据,也为乳腺癌及其转移的治疗提供潜在的靶点。Exo70,the key subunit of Exocyst protein complex,promotes cell metastasisby self-dimerization or by involving in the Exocyst complex formation.In this research,we found that Exo70 was dramatically more capable of forming self-dimerization than binding to the other subunits of exocyst complex,and aindicative of the important roles of its self-dimerization.Then we constructed a set of truncation mutants of Exo70 in order to determine the core sequence for its dimerization.With co-immun oprecipitation analysis,we identified two identical protein sequences"SLEKS"located at amino acid 31~35 and 505~509 as critical sequencesfor the dimerization of Exo70.By "cell transwell"essay,we further found that the full length Exo70 significantly promotes breast cancer metastasis,while the two truncation mutants of Exo70(Δ31~35 and Δ505~509) don't process such ability,indicating that these two "SLEKS"sequences play pivotal role in breast cancer cell metastasis and invasion.Together,these results provide fundamental basis for researches on novel functions of Exo70 besides its well-known role in Exocyst complex formation and provide potential drug target for treating breast cancer and its metastasis.国家自然科学基金青年项目(31300970);; 福州大学科研启动项目(510402)共同资

A Novel Path to Patterning Based on the Static Breath Figure Technique

李磊:2001年7月-2003年6月，在北京大学化学与分子工程学院高分子系从事博士后研究。[中文文摘]对呼吸图技术制备二维有序多孔结构的研究进展进行了综合评述,并重点介绍了本课题组发展的静态呼吸图技术.利用静态呼吸图法,可制备高度有序的聚合物、聚合物/无机物微孔膜.这些有序的结构可以直接应用于光掩膜.进一步,多孔聚合物膜可以被紫外光交联和改性.表面改性的多孔聚合物膜可以用于细胞支架.而交联的聚合物/无机物前驱体微孔膜可以用来制备无机纳米材料阵列.结果表明,静态呼吸图技术是一种简单、高效的对聚合物、聚合物/无机物薄膜进行图案化的通用方法,并展示了图案化薄膜广阔的功能化前景.[英文文摘]The breath figure technique is one of the most promising strategies for the fabrication of large-sized patterns containing an ordered two-dimensional array of holes.In this review,particular emphasis is placed on the static breath figure technique,which is a robust methodology suitable for various polymers including linear polystyrene (PS),amphiphilic diblock copolymers and polymer/inorganic precursor hybrid films.The resultant highly ordered microporous polymer films can be used as a mask for lithography.Moreover, the microporous polymer films can be surface modified and cross-linked by UV irradiation. The surface modified films can potentially be applied as a cell scaffold and the cross-linked polymer/hybrid films can be used as templates for the growth of nanomaterial arrays.国家自然科学基金(50703032,20974089); 福建省杰出青年基金(2009J06029);教育部新世纪人才基金项目资助

Experimental Study of the Abalone Shell on Cataract induced by Oxidative Stress

目的研究石决明提取物对体外培养的晶状体氧化应激性白内障形成的作用及机制。方法离体培养小鼠晶状体,应用不同浓度的石决明提取物预孵育晶状体24h后,加入1mm过氧化氢,继续培养3小时后恢复正常培养,72小时后观察小鼠晶状体混浊程度,测定晶状体组织培养液中的乳酸脱氢酶(LDH)含量,晶状体组织中还原型谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)活力。结果石决明提取物在1~2 mg/ml浓度范围内减轻氧化应激造成的晶状体混浊,减少晶状体LDH的释放,提高组织内GSH含量和SOD活力。结论石决明提取物可减轻氧化应激白内障的形成,其作用主要与石决明提取物提高内源性抗氧化系统有关。Objective To study the effect of the abalone shell extract on oxidative stress induced cataract formation and its mechanism in cultured mouse lens in vitro. Methods The cultured mouse lens were pretreated with the abalone shell extract in different concentrations for24 hours,and then 1mm hydrogen peroxide was added and continued incubating for 3 hours,and they were changed to normal culture media.After 72 hours,the opacity of each lens was observed under an anatomical microscope,the content of lactate dehydrogenase( LDH)leakages,the content of the reduced glutathione( GSH) and activity of superoxide dismutase( SOD) in lens tissue were assayed. Results Abalone shell extract in the concentration range of 1 ~ 2 mg / ml reduced the lens opacity caused by oxidative stress,alleviated the release of LDH,and increased GSH content and SOD activity in cultured lens. Conclusion Abalone shell extract can alleviate the oxidative stress induced cataract formation,and this effect is mainly related to its improvement of the endogenous antioxidant system in lens.2012年福建省卫生厅中医药科研专项课题(No.WST201210);; 2013年福建省卫生厅中医药科研专项课题(No.wzhw201302);; 2014年厦门市科技局科技惠民项目课题(No.3502Z20144030

粗糙铂电极上甲酸吸附氧化的电化学原位表面增强拉曼光谱研究

采用循环伏安法和电化学原位表面增强拉曼光谱 (SERS)技术研究甲酸的解离吸附与氧化行为 .首次报道了甲酸吸附、解离和氧化的电化学原位SERS谱 ,发现甲酸在粗糙铂电极上能自发解离吸附 ;首次成功地获得了粗糙铂电极上甲酸吸附解离的强吸附中间体CO和活性中间体COOH的表面增强拉曼光谱 ,同时首次检测到甲酸氧化最终产物CO2 的拉曼光谱信号 ,从分子水平证实甲酸解离吸附反应的双途径机理

贝壳吸声界面吸声性能试验研究

以贝壳、玻璃棉为主要材料,利用贝壳、玻璃棉、空腔组成一个吸声界面,通过驻波管吸声降噪的试验研究,探究不同厚度的贝壳层、玻璃棉层、空腔这三者的搭配组成的吸声界面的吸声频谱特性及变化规律,用于不同贝壳层厚度、不同玻璃棉厚度吸声性能的预测评估,为最终实现贝壳吸声产品研发提供参考

In-situ SERS study on the electro-oxidation with HCOOH on a roughened platinum electrode

The dissociative adsorption and oxidation behavior of HCOOH on Pt was investigated by cyclic voltammogram (CV) and in-situ surface enhanced Raman spectroscopy (SERS) techniques. The in-stiu SERS of HCOOH adsorption, dissociation and oxidation on rough Pt is reported. It is found that HCOOH can spontaneously dissociate. The surface Raman spectra of CO, the strongly adsorbed intermediate and COOH, the weakly adsorbed intermediate of the dissociative adsorption of HCOOH were successfully obtained for the first time. At the same time, the Raman spectra of the finally oxidized product CO2 of HCOOH was also firstly detected. The dual path reaction mechanism for the oxidation of HCOOH was confirmed at molecular level

Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model

阿尔茨海默病（Alzheimer’s Disease, AD）是一种以渐进性认知功能丧失为主要特征的神经退行性疾病，是最为常见的老年痴呆类型。随着全球人口老龄化的加剧，AD正在成为二十一世纪最大的疾病之一。该研究首次揭示sTREM2在AD中具有重要的保护功能，提出sTREM2或可用于AD治疗的新观点，同时也进一步佐证了小胶质细胞在AD治疗中的核心作用，研究为AD等神经退行性疾病的防治开辟了新思路、提供了新靶点。 厦门大学医学院博士后钟力和硕士研究生徐颖为论文共同第一作者，陈小芬教授和卜国军教授为该论文的共同通讯作者。厦门大学的文磊、孙灏、卓仁恭等教授和美国Sanford-Burnham-Prebys医学研究所的许华曦教授共同参与了该项目的研究。【Abstract】Triggering receptor expressed on myeloid cells 2 (TREM2) is a microglial surface receptor genetically linked to the risk for Alzheimer’s disease (AD). A proteolytic product, soluble TREM2 (sTREM2), is abundant in the cerebrospinal fluid and its levels positively correlate with neuronal injury markers. To gain insights into the pathological roles of sTREM2, we studied sTREM2 in the brain of 5xFAD mice, a model of AD, by direct stereotaxic injection of recombinant sTREM2 protein or by adeno-associated virus (AAV)-mediated expression. We found that sTREM2 reduces amyloid plaque load and rescues functional deficits of spatial memory and long-term potentiation. Importantly, sTREM2 enhances microglial proliferation, migration, clustering in the vicinity of amyloid plaques and the uptake and degradation of Aβ. Depletion of microglia abolishes the neuroprotective effects of sTREM2. Our study demonstrates a protective role of sTREM2 against amyloid pathology and related toxicity and suggests that increasing sTREM2 can be explored for AD therapy.Research by the authors was supported by grants from the National Natural Science Foundation of China 81370459, 31400914 (to X.C.), 81701079 (to L.Z.), 81373999, 81774377 (to L.W.), and 81601227 (to R.Z.), grants from the Natural Science Foundation of Guangdong Province 2016A030306005 (to X.C.), 2016A030310371 (to R.Z.), grants from the Fundamental Research Funds for the Central Universities 20720180055 (to X.C.), grants from the Alzheimer's Association AARG-18-56635 (to X.C.), and C4C-15-369446 (to H.X.). NIH grants RF1AG056130 (to G.B. and H.X.), R01AG035355 (to G.B.), R37AG027924 (to G.B.), and RF1AG056114 (to H.X.), grants from the Postdoctoral Science Foundation of China 2016M600503 and 2017T100469 (to L.Z.), a grant from the Tanz Family Funds (to H.X.), and a grant from the Natural Science Foundation of Fujian Province 2016J05203 (to R.Z.).该工作得到国家自然科学基金、厦门大学校长基金、广东省自然科学杰出青年基金、美国阿尔茨海默氏症协会基金和中国博士后科学基金等的资助

Fabrication of multi-level carbon nanotube arrays with adjustable patterns

通讯作者地址: Li, L (通讯作者),Xiamen Univ, Coll Mat, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Mat, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Xiamen, Peoples R China 3. Natl Inst Adv Ind Sci & Technol, Next Generat Device Team, Res Ctr Photovolta Technol, Tsukuba, Ibaraki 3058568, Japan 电子邮件地址: [email protected] carbon nanotube (CNT) arrays with adjustable patterns were prepared by a combination of the breath figure (BF) process and chemical vapor deposition. Polystyrene-b-poly(acrylic acid)/ferrocene was dissolved in carbon disulfide and cast onto a Si substrate covered with a transmission electron microscope grid in saturated relative humidity. A two-level microporous hybrid film with a block copolymer skeleton formed on the substrate after evaporation of the organic solvent and water. One level of ordered surface features originates from the contour of the hard templates; while the other level originates from the condensation of water droplets (BF arrays). Ultraviolet irradiation effectively cross-linked the polymer matrix and endowed the hybrid film with improved thermal stability. In the subsequent pyrolysis, the incorporated ferrocene in the hybrid film was oxidized and turned the polymer skeleton into the ferrous inorganic micropatterns. Either the cross-linked hybrid film or the ferrous inorganic micropatterns could act as a template to grow the multi-level CNT patterns, e. g. isolated and honeycomb-structured CNT bundle arrays perpendicular to the substrate.National Natural Science Foundation of China 50703032 51035002 20974089 Ministry of Education of China NCET-08-0475 Natural Science Foundation of Fujian Province 2009J0602

Honeycomb-Patterned Hybrid Films and Their Template Applications via A Tunable Amphiphilic Block Polymer/Inorganic Precursor System

通讯作者地址: Li,L(通讯作者),Xiamen Univ,Coll Mat, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Mat, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 3. Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 电子邮件地址: [email protected], we show a facile and versatile method-prepare highly ordered inorganic patterns on solid substrates by pyrolyzing UV cross-linked polymr/functional. precursor hybrid films. The crosslinked polymer matrix acted as structure-directing agent in a pyrolyzing process, whereas the functional precursor was converted into the skeleton of the micropatterns. The inorganic micropatterns could be further catalytically functionalized to grow CNT and ZnO nanorod arrays by simply changing different functional precursors. This simple technique offers new prospects in the field of micropatterns, nanolithography, and template.National Natural Science Foundation of China,50703032,20974089;Natural Science Foundation of Fujian Province,2009J06029; Ministry of Education of Chin