Progress in the studies of keratinocyte growth factor

角质细胞生长因子(KGF)从属于成纤维细胞生长因子家族。KGF基因表达受多种细胞因子调控。KGF与受体KGFR特异性的结合发挥其多种生物学功能:参与组织、器官的发育;参与皮肤、胃、肠、肾、膀胱、肺等上皮的损伤修复;减少放、化疗所带来的副作用,具有损伤防护功能;KGF与肿瘤密不可分。Keratinocyte growth factor (KGF) is a member of the fibroblast growth factor family. A lot of cytokinesare responsible for KGF expression. By binding with its receptor KGFR, KGF does its biological functions in repairprocesses of various tissues and organs, including skin, bowel, bladder, kidney, lung and so on. KGF can stimulatedevelopment of some tissues and organs. It also acts as a protective factor on injury induced by radiation andchemotherapy. KGF is concerned with some tumors.福建省自然科学基金资助项目(B0210002

Research on Perception of Wuxi Huishan Ancient Town Destination Image Based on IPA Analysis

运用IPA分析法对无锡惠山古镇旅游目的地形象感知进行了实证分析。研究表明:惠山古镇目的地形象与游客期望总体上还存在差距,古建筑、文物古迹、空气质量等是古镇形象目前的相对优势,而原生态生活、河水质量、旅游服务以及公共厕所等是古镇形象目前的相对劣势,是今后形象建设的重点。在调查基础上提出了针对性建议,尤其是要刻意地将惠山祠堂群与古镇标志性景观和古镇特色结合起来进行宣传

Cloning of Recombinant Keratinocyte Growth Factor and Its Construction of Plant Expression Vector

从鼠肺中提取RNA,设计相应的引物,经RT PCR扩增出角化细胞生长因子cDNA序列;插入pMD18 T载体,经DNA测序证实为KGF基因.将KGF基因经酶切后连接于植物CaMV35S启动子和NOS终止子之间,单酶切插入pBI1301植物双元表达载体,转化大肠杆菌,阳性克隆提取质粒后转化农杆菌EHA105,实现KGF植物表达载体的构建,为KGF的进一步研究奠定基础.Abstract: The cDNA gene encoding keratinocyte growth factor (KGF) was amplified from mouse lung by RT-PCR and inserted into pMD18-T vector. The result of automatic sequence showed that the sequence of inserted fragment was KGF gene. The KGF gene was inserted into pBPFΩ7 under the control of CaMV35S promotor and NOS terminator, then was digested and inserted into pBI 1301 to form the plant binary expression vector. The plant binary expression vector of KGF was transformed into Agrobacterium EHA 105. It provided a reliable basis for the further study on KGF.福建省自然科学基金(B0210002)资助(2002年

Cloning and Secquencing Analysis of Calmodulin Gene from Sonneratia apertala of Mangrove Plant

用高等植物钙调蛋白的保守序列设计两端引物 ,以红树植物海桑属无瓣海桑Sonneratiaapertala总DNA为模板 ,扩增出无瓣海桑钙调蛋白基因 ,并以之构建重组质粒pT ACaM .经测序分析比对 ,发现无瓣海桑钙调蛋白基因全长 1418bp ,其中第 1外显子 76bp ,第 2外显子 371bp ,中间为一 946bp的内含子所隔开 .编码 148氨基酸的蛋白质 ,其编码区序列与已知的其他高等植物的钙调蛋白基因核苷酸序列同源性高达 85 %以上 .而蛋白质序列同源性达95 %以上The sequence was amplified using total DNA of Sonneratia apertala as the template.The PCR primers was designed and synthesized according to the 5′-and 3′-terminal oligo nucleotide sequences of calmodulin gene of plants in Genbank. The result of DNA sequence analysis shows that the gene is 1 418 bp length which contains 2 exons. The first one is 76 bp, the second is 731 bp. They are interrupted by a 946 bp intron. The nucleotide sequences of ORF share more than 85% homologies as compared with those of calmodulin genes of other several plants, the amino acid sequences share more than 95% homologies with it

乡村旅游拉力动机的结构及旅游者类型分析

在乡村旅游研究领域,基于动机性质分类视角的研究起步较晚,研究者更多关注推力动机,对拉力动机研究较为忽视。采用因子分析得出乡村旅游拉力动机由乡村文化、农事活动、乡村环境和乡村娱乐4个维度构成,乡村文化和农事活动是最为重要的动机。聚类分析得出男女样本中均存在活跃型、真实体验型、环境型和其他型旅游者,男性中单独存在娱乐型、而女性中单独存在享受型旅游者,表征了位于三个不同发展层面的旅游者群体。旅游者的乡村旅游动机正向深度体验发展。在讨论基础上给出营销建议

The structure of pull motivations of rural tourism and the segmentation of rural tourists

在乡村旅游研究领域,基于动机性质分类视角的研究起步较晚,研究者更多关注推力动机,对拉力动机研究较为忽视。采用因子分析得出乡村旅游拉力动机由乡村文化、农事活动、乡村环境和乡村娱乐4个维度构成,乡村文化和农事活动是最为重要的动机。聚类分析得出男女样本中均存在活跃型、真实体验型、环境型和其他型旅游者,男性中单独存在娱乐型、而女性中单独存在享受型旅游者,表征了位于三个不同发展层面的旅游者群体。旅游者的乡村旅游动机正向深度体验发展。在讨论基础上给出营销建议