Effects of Wu Ling Powder on Blood Pressure and Renin Angiotensin Aldosterone System in Spontaneously Hypertensive Rats

目的:观察五苓散降压作用及其对肾素-血管紧张素-醛固酮系统(RAAS)的影响。方法:选择50只自发性高血压大鼠按随机数字表法分为模型组、五苓散高、中、低剂量组、替米沙坦组各10只,选择10只Wistar京都大鼠作为正常组,测量大鼠用药前后2、4、6、8周的血压、心率以及体质量。测定给药8周各组血清中肾素(Renin)、血管紧张素Ⅱ(AngⅡ)、醛固酮(ALD)水平以及心肌组织中血管紧张素Ⅱ1型受体(AT1)、ACE2血管紧张素转化酶2(ACE2)mRNA的表达。结果:用药后第4周起五苓散各剂量组对自发性高血压大鼠的血压、心率以及体质量均有降低作用;用药8周后,五苓散高、中剂量组血清中Renin、AngⅡ、ALD的含量均低于模型组,五苓散各剂量组心肌组织中AT1 mRNA的表达量低于模型组,ACE2 mRNA的表达量高于模型组。结论:五苓散能够降低血压,其机制可能与RAAS系统的调控作用有关。Objective: observing the effect of Wuling Powder on Blood Pressure and the Renin-Angiotensin-Aldosterone System( RAAS). Methods: 50 spontaneously hypertensive rats were randomly divided into model group,Wuling Powder of high dose group,medium dose group,low dose group,Telmisartan group,with 10 rats in each group. The normal group was10 Wistar-Tyoto rats. The rats were measured before the treatment and after 2,4,6,8 weeks for blood pressure,heart rate and weight. After 8 weeks,the content of Renin,AngⅡ and ALD in the blood serum of rats and the expression of mRNA of AT1 and ACE2 in muscle tissue were tested. Results: After treatment for fourth weeks,Wuling Powder in each dose group can reduce blood pressure heart rate and weight were significantly. After 8 weeks,Wuling Powder in high dose group and medium dose group have less Renin,AngⅡ and ALD than the model group. Wuling Powder in each dose group of myocardial tissue in AT1 mRNA was lower than that of model group and ACE2 mRNA was higher than that of model group.Conclusion: Wuling Powder can reduce blood pressure in spontaneously hypertensive rats,its mechanism may be related to the regulation of RAAS.2013~2015福建省中医药科研项目计划(wzln201309)-五苓散健脾-利水-温阳通脉多靶点途径调控高血压大鼠RAAS表达的机制研

芪参益气滴丸对ApoE基因敲除小鼠动脉粥样硬化中调节性T细胞的影响

目的:研究芪参益气滴丸对动脉粥样硬化中调节性T细胞的影响。方法:采用6周龄雄性C57BL/6J小鼠和Apo E基因敲除小鼠,分为正常组、模型组、芪参低剂量组、芪参高剂量组,每组6只。除正常组外,其他3组小鼠均给予高脂饲料喂养,药物组还给予高、低剂量的芪参益气滴丸,8周后评价内脏指数、动脉粥样硬化病变、调节性T细胞情况。结果:与模型组比较,芪参高剂量组可显著降低肝指数(P<0.05),减少动脉粥样硬化斑块面积(P<0.05,P<0.01),并增加脾脏调节性T细胞数量(P<0.01)。结论:芪参益气滴丸可能通过增加脾脏调节性T细胞数量抑制动脉粥样硬化形成。福建省自然科学基金项目(No.2016J01413);;广东省自然科学基金项目(No.2016A030313860);;教育部留学回国人员科研启动基金项目(No.2015-311);;教育部“新世纪优秀人才支持计划”项目(No.NCET-13-0505);;国家重点研发计划“重大慢性非传染性疾病防控研究”重点专项(No.2016YFC1305903)~

Efficient Preparation of β-O-4 Dilignols and Their Oxidative Coupling Studies

Efficient Preparation of β-O-4 Dilignols and Their Oxidative Coupling Studie

Fueling metabolism one reaction at a time

Fueling metabolism one reaction at a tim

设计依赖非天然辅酶的氧化还原代谢途径

Designing non-natural redox cofactor-wired metabolis

Systems Analysis and engineering of Oleaginous Red Yeasts

Systems Analysis and engineering of Oleaginous Red Yeast

Conversion of biomass to microbial lipids for biofuels

Conversion of biomass to microbial lipids for biofuel

Non-natural redox cofactor-wired metabolic circuits

Non-natural redox cofactor-wired metabolic circuit

Efficient acid-catalyzed hydrolysis of cellulose in ionic liquid

A novel method for cellulose hydrolysis catalyzed by mineral acids in the ionic liquid 1-butyl-3-methylimidazolium chloride ([C(4)mim]Cl) has been developed that facilitates the hydrolysis of cellulose with dramatically accelerated reaction rates at 100 degrees C under atmospheric pressure and without pretreatment