Abundance and production of bacteria and their correlations with environmental factor

以2014年8月南海北部海水样品为研究对象,利用平板计数法和流式细胞仪计数法对南海北部表层和垂直海域可培养细菌和细菌总数分布状况进行研究,对细菌; 生产力进行测定,并结合环境因子进行相关性分析。结果表明:珠江口到南海北部海域,水平方向可培养细菌总数变化范围是3.70*10~2 ~; 1.42*10~3 CFU/mL,细菌总数变化范围是5.12 * 10~5 ~1.61 * 10~6; cells/mL,细菌生产力的变化范围是0.03 ~0.40 mg/m~3/h;垂直方向上可培养细菌变化范围是1.08 *10~3 ~9.00; *10~3 CFU/mL,细菌生产力变化范围是0.01 ~0.08; mg/m~3/h,其中表层海水中的细菌生产力明显高于底层。与环境因子相关性分析表明,水平方向上,影响南海北部表层海水细菌总数和细菌生产力的主要因; 素是温度、盐度、硝酸盐(NO_3-N)、硅酸盐(SiO_3-Si)、亚硝酸盐(NO_2-N)和磷酸盐(PO_4-P)(P<0.05);垂直方向上; ,影响南海北部可培养细菌总数的主要因素是NO_2-N(P<0.05),影响细菌生产力的主要影响因素是温度和盐度(P; <0.05)。可见,南海北部表层海水中细菌总数高于可培养细菌总数2; ~3个数量级,表明该海域表层海水存在大量不可培养细菌;细菌的生命活动在海水表层相较底层更为活跃。Distribution of abundance and production of bacteria and their; correlations with environmental factor were investigated, using plate; count method and flow cytometry, in the surface and vertical waters of; the northern south China sea in August 2014. The cultivable bacterial; abundance ranged from 3.70 * 10~2 CFU/mL to 1.42 * 10~3 CFU/ mL, the; total bacterial abundance ranged from 5.12 * 10~5 cells/mL to 1.61 *; 10~6 cells/mL and the bacterial productions varied from 0.03 mg/m~3/h to; 0.40 mg/m~3/h in horizontal distribution. In vertical distribution, the; cultivable bacterial abundance ranged from 1.08 * 10~3 CFU/mL to 9.00 *; 10~3 CFU/mL and the bacterial productions varied from 0.01 mg/m~3/h to; 0.08 mg/m~3/h, respectively. The correlation analysis results showed; that the environmental factors affecting the abundance of total bacteria; and bacterial productivity included temperature, salinity,; nitrate(NO_3-N),silicate (SiO_3-Si), nitrite (NO_2-N),and phosphate; (PO_4-P) (P<0.05). NO_2-N was the main influencing factor to cultivable; bacteria abundance (P < 0.05), while bacterial productivity was highly; correlated with temperature and salinity in vertical distribution of; northern south China sea (P < 0.05). The total bacterial abundance was 2; ~ 3 orders of magnitude higher than cultivable bacteria, suggesting that; there were many uncultured bacterium in surface water of northern south; China sea and bacterial activities in the surface maybe more active than; in the bottom.全球变化与海气交互作用; 国家重点研发计划全球变化及应对专项; 海洋公益性行业科研专

Diversity of CO_2 fixation gene in the surface waters of northern South China Sea in the Calvin cycle

为揭示南海北部表层海水中参与卡尔文循环的固碳基因多样性及其与环境因子的关系,本研究以卡尔文循环中的关键酶核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubis CO)的Ⅰ、Ⅱ型基因(cbbL、cbbM)作为分子标记,采用Illumina Miseq高通量测序技术对海水中的固碳基因多样性进行分析,并结合多元统计分析的方法,探讨了固碳基因多样性与环境因子的关系.结果显示,在南海北部表层海水中,含cbbL基因固碳基因主要归属于变形菌门(Proteobacteria)、蓝藻门(Cyanobacteria)和厚壁菌门(Firmicutes),其中,优势亚群分别是γ-变形菌亚门(45.3%)、蓝细菌(30.9%)和β-变形杆菌亚门(23.8%);而含cbbM基因的固碳菌群未检测到;近岸的A9站位与其他站位物种组成有一定的差异,异着色菌属(Allochromatium)、硫杆菌属(Thiobacillus)和硫单胞菌属(Thiohalomonas)为其特有菌属.相关性和冗余分析(RDA)结果显示,含cbbL基因的固碳基因丰度与水温、盐度呈显著负相关(p<0.01),与硝酸盐、亚硝酸盐、硅酸盐呈显著正相关(p<0.01).To elucidate the diversity of CO_2 fixation and its relationship with environmental factors in surface water of northern South China Sea,the coding gene form I( cbbL) and form II( cbbM)( that encodes Ribulose-1,5-biphosphate carboxylase/oxygenase( Rubis CO) were chosen as biomarkers in Calvin-Benson-Bassham cycle( a common carbon fixation pathway for chemolithoautotrophic microorganisms) to analyze the carbon sequestration gene diversity. The Illumina Miseq sequencing method and multivariate statistical analysis were employed. The cbbM gene was not detected in any of samples,while the cbbL gene was found in all samples. The cbbL-containing genetic communities were dominated by Proteobacteria,Cyanobacteria and Firmicutes,in which γ-Proteobacteria( 45. 3%),Cyanobacteria( 30. 9%),β-Proteobacteria( 23. 8%) were predominant subpopulation. There were differences between the nearshore station of A9 with other stations,in which Allochromatiu,Thiobacillus and Thiohalomonas were unique species in A9 station.Relativity and redundancy analysis suggest that water temperature, salinity, nitrate, nitrite, silicate were highly statistically significant factors.Temperature and salinity were positively( p < 0. 01),while the other three factors negatively influenced CO_2 fixation gene of cbbL gene abundance( p <0. 01).全球变化与海气交互作用专项(No.GASI-03-01-02-05);; 海洋公益性行业科研专项(No.201305030,201405007)~

Clone of OsCHX1 Gene and Agrobacterium Mediated Transformation of the Gene into Rice

利用RT-PCR扩增水稻的Na+、K+/H+反向转运蛋白(OsCHX1)基因全序列,将其与35S启动子连接后,插入到p1301中,构建植物过量表达载体p1301-35S-OsCHX1-NOS.将该质粒转化农杆菌EHA105,并对水稻愈伤组织进行转化,获得了再生植株.对再生植株进行GUS和PCR鉴定,发现超过85%的再生植株为阳性植株.此研究结果为进一步探讨OsCHX1基因功能提供了实验材料.The full length of OsCHX1 gene was amplified by RT-PCR and pBPF-35S-OsCHX1 was constructed after ligating gene of OsCHX1 with pBPF.The Hind III digested fragment from pBPF-35S-OsCHX1 was inserted into p1301 and p1301-35S-OsCHX1-NOS was constructed.OsCHX1 gene was introduced into rice(Oryza Sativa Japonica cultivar Npponbare) by Agorbacterium mediated method.More than 100 seedlings regenerated from transformants were obtained.After detection by GUS stain and PCR it was found that OsCHX1 gene was transfered into rice genomes.福建省青年人才创新项目(2005J004)资

Study on Introducing KGF Gene into Rice Mediated by Agrobacterium

角质细胞生长因子(Keratinocyte growth factor,KGF)在组织的损伤修复中起着重要的作用.在植物里面表达高活性的KGF具有成本低、易开发的优点.本实验将人角质细胞生长因子基因经农杆菌介导转入水稻中,获得110株再生植株.对再生植株进行GUS检测发现有44.7%植株呈阳性,PCR检测的结果也显示有52.6%的植株为阳性植株.Keratinocyte growth factor(KGF) plays an important role in the wound repair.It is economical to express KGF in plant.In this study,we introduced human KGF gene into rice mediated by Agrobacterium.110 independent seedlings regenerated from transformants have been obainted.The positive ratio of the seedling is 44.7% by GUS detection and 52.6% by PCR detection

The Construction of a Plant Binary Vector pCAMBIA130-pRP-6-IPT-Nos for Expressing IPT Gene in Developing Rice Seed

以含有IPT基因的中间载体pSG516为基础,采用酶切的方法获得IPT-Nos核酸片段,以水稻品种9311为材料,采用PCR的方法克隆出种子中特异表达的醇溶谷蛋白RP-6基因启动子,并将此启动子连接到pCAMBIA1300上,构建pCAMBIA1300-pRP-6载体,将IPT-Nos核酸片段插入到pCAMBIA1300-pRP-6,构建了pCAMBIA1300-pRP-6-IPT-Nos双元表达载体.In order to obtain transgenic rice which express exogenous IPT gene in developing seed and to further investigate the function of cytokinin in rice seed development,we constructed the binary vector pCAMBIA1300-pRP-6-IPT-Nos.Firstly IPT-Nos fragment was obtained from pSG516 by cutting PSG516 plasmid with Nco I and Spe I restriction enzymes.Secondly,developing endosperm RP-6 gene specifically expressed in developing endosperm was selected and it′s promoter(pRP-6) was isolated from rice genomic DNA(cultivar 9311) by using PCR amplification.Thirdly pRP6 was inserted into pCAMBIA1300,with pCAMBIA1300-pRP-6 obtained.Finally,plant expression vector pCAMBIA130-pRP-6-IPT-Nos was constructed after inserting IPT-Nos fragment into pCAMBIA1300-pRP-6.福建省青年科技人才创新项目(2005J004)资

施用氮肥对人工羊草草地产量及养分吸收的影响

为研究施氮对人工羊草草地产量及养分吸收的影响.以中科2号羊草(Leyrnus chinensis‘ghongke No.2')为材料,设置5个氮肥处理水平,测定不同生育期羊草氮、磷、钾养分含量以及干草产量。结果表明:施用氮肥可以显著提高羊草产量,施用量以133.8 kg·hm~(-2)最为适宜,羊草产量为13134.7 kg·hm~(-2),每千克纯氮增产55.5 kg;施用氮肥可以提高羊草氮、磷、钾吸收量

定量遥感反演作物蒸腾和土壤水分利用率的区域分异

首先讨论了在农业生态站建立的行之有效的、以遥感数据为主体的作物蒸腾二层模型.重点阐明了以多时相辐射温度和热惯量信息为基础的作物地表混合像元（视场）温度的分解模型,在遥感反演过程中提出了一些在模型和算法方面的优化和改进:提出了一个区域比辐射率估算的算法、提出了运用地表温度的静态反馈算法和扩展生态台站的空气温度到区域的算法.并在LUCC分类技术的支持下,提出了以地面粗糙度和辐射温度为参数的地面2m高的气温和风速的空间扩展算法.最后应用 NOAA-AVHRR和地面同步观测及其定标,按像元逐点运算,反演了华北地区农田作物蒸腾和水分利用率的区域分布.揭示了春天华北地区水资源在农业上的利用效率,为采取有效的节水措施提供了科学依据

All solution-processed large-area patterned flexible photodetectors based on ZnOEP/PVK hybrid film

All solution-processed large-area patterned flexible photodetectors were successfully fabricated by utilizing small-molecule organic semiconductor/polymer hybrid film as an active layer and patterned printed Ag electrodes. The large-area hybrid film could be fabricated by a direct solution-process self-assembly method and had a wide absorption spectrum and improved charge transport ability, resulting in high photoresponsivity. Moreover, the device displayed fast response time, high stability and broadband spectral response. Importantly, the device exhibited high flexibility, good folding strength and electrical stability on shafts with different curvature radiuses and after bending. Even after 500 bending cycles, the device still showed good photodetector and photoswitching properties. The facile low-cost large-area fabrication process and excellent performance gives potential applications in large-scale flexible electronic devices