利用RT-PCR扩增水稻的Na+、K+/H+反向转运蛋白(OsCHX1)基因全序列,将其与35S启动子连接后,插入到p1301中,构建植物过量表达载体p1301-35S-OsCHX1-NOS.将该质粒转化农杆菌EHA105,并对水稻愈伤组织进行转化,获得了再生植株.对再生植株进行GUS和PCR鉴定,发现超过85%的再生植株为阳性植株.此研究结果为进一步探讨OsCHX1基因功能提供了实验材料.The full length of OsCHX1 gene was amplified by RT-PCR and pBPF-35S-OsCHX1 was constructed after ligating gene of OsCHX1 with pBPF.The Hind III digested fragment from pBPF-35S-OsCHX1 was inserted into p1301 and p1301-35S-OsCHX1-NOS was constructed.OsCHX1 gene was introduced into rice(Oryza Sativa Japonica cultivar Npponbare) by Agorbacterium mediated method.More than 100 seedlings regenerated from transformants were obtained.After detection by GUS stain and PCR it was found that OsCHX1 gene was transfered into rice genomes.福建省青年人才创新项目(2005J004)资
