The metabolic profilings study of serum and spinal cord from acute spinal cord injury rats ^1H NMR spectroscopy

目的：采用-1H NMR核磁共振代谢组学的方法研究急性脊髓损伤模型大鼠的代谢组学特征及生物标志物,探讨核磁共振代谢组学应用于脊髓损伤研究的可行性。方法：取8周龄清洁级雄性SD大鼠20只,体重（200±10）g,按照随机数字法分为假手术组和模型组,每组10只,模型组采用改良的Allens法制作急性脊髓不完全损伤模型,假手术组不损伤脊髓,术后第1、5、7天采用BBB运动功能评分法进行行为学观察,术后第7天收集脊髓组织作病理学观察,核磁共振代谢组学对两组大鼠血清和脊髓样本进行代谢组学分析。结果：BBB评分显示假手术组术后后肢运动无明显改变,各时间点差异无统计学意义,模型组大鼠术后双下肢呈迟缓性瘫痪,BBB运动评分较低,各时间点差异存在统计学意义,两组运动功能评分在各时间点的差异均有统计学意义;病理切片显示假手术脊髓结构正常,神经分布均匀,模型组脊髓组织结构紊乱,神经元数目减少,存在炎性细胞浸润和空腔坏死组织。代谢组学分析表明,血清中极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）、谷氨酰胺（glutamine）、柠檬酸（citrate）、二甲基甘氨酸（DMG）等物质和脊髓中谷胱甘肽（glutathione）、3-羟基丁酸（3-OH-butyrate）、N-乙酰天冬氨酸（NAA）、磷酸胆碱（GPC）、谷氨酸（glutamate）、抗坏血酸（ascorbate）等物质浓度有明显变化（P〈0.05）。结论 ：通过对假手术组和模型组大鼠血清和脊髓样本进行代谢组学检测和分析得到了两组样本的差异性代谢物质,有助于解释急性脊髓损伤后血清和脊髓组织中的特异性小分子物质的变化规律,为后期针对性地研究这些代谢标记物在急性脊髓损伤中的作用提供研究基础。Objective： To establish the rat model of acute spinal cord injury,followed by aprimary study on this model with 1H NMR based on metabonomics and to explore the metabonomics and biomarkers of spinal cord injury rat.Methods： Twenty eight-week-old adult male SD rats of clean grade,with body weight of （200±10） g,were divided into sham operation group and model group in accordance with the law of random numbers,and every group had 10 rats. The rats of sham operation group were operated without damaging the spinal cord,and rats of model group were made an animal model of spinal cord incomplete injury according to the modified Allen＇s method. According to BBB score to observate the motor function of rats on the 1th,5th,and 7th days after surgery. Postoperative spinal cord tissue was collected in order to pathologic observation at the 7th day,and the metabolic profilings of serum and spinal cord from spinal cord injury rats were studied by 1H NMR spectroscopy.Results： The hindlimb motion of rats did not obviously change in sham operation group,there was no significant difference at each time point;and rats of model group occurred flaccid paralysis of both lower extremities,there was a significant difference at each time; there was significant differences between two groups at each time. Pathological results showed the spinal cord structure was normal with uniform innervation in shame group,while in model group,the spinal cord structure was mussy,and the neurons were decreased,with inflammatory cells and necrotic tissue. Analysis of metabonomics showed that concentration of very low density fat protein （VLDL）,low density fat protein （LDL）,glutamine,citric acid,dimethylglycine （DMG） in the serum and glutathione,3-OH-butyrate,N-Acetyl-L-aspartic acid （NAA）,glycerophosphocholine （GPC）,glutamic acid,and ascorbate in spinal cord had significant changes（P〈0.05）.Conclusion： There are significant differences in metabolic profile from serum and spinal cord sample between model group and sha浙江省自然基金（编号：LY15H270003）;浙江省中医药科技计划项目（编号：2015ZZ017

一种有色废液的脱色处理方法

本发明公开了一种有色废液的脱色处理方法，采用负载的金属催化剂与还原剂可将有色废液快速脱色，其脱色过程不消耗负载的金属催化剂，只需添加还原剂可保持持续不断的有色废液脱色处理。负载的金属催化剂失活后，可采用有机溶剂或水清洗脱除催化剂表面附着的污染物，负载的金属催化剂可重新具有活性，与还原剂一起快速脱除有色废液的颜色。此有色废液的脱色处理方法过程简单，所有原料价格低廉，处理有色废液量大，易于规模化应用，而且负载的金属催化剂可循环长期利用，大幅降低有色废液的脱色处理成本

一种重金属离子去除剂的制备方法及应用

本发明公开了一种重金属离子去除剂的制备方法及应用。所述的重金属离子去除剂，负载在低廉的载体表面，具有较大的比表面积，对重金属离子的去除率高，容量大，并且环境友好，可用于去除地下水、地表水等各类水体中铅、镉、砷、铬、铜、镍、钴、锌等重金属离子污染物。此重金属离子去除剂可以再生，循环利用，降低处理成本

一种负载的金属催化剂的制备方法

本发明公开了一种负载的金属催化剂的制备方法及应用，其中金属催化剂包括载体骨架材料和负载在载体骨架材料表面的金属或金属氧化物的纳米颗粒，或螯合在载体骨架材料表面的金属离子，其中起催化作用的金属离子、金属或金属氧化物的纳米颗粒是通过粘结在载体骨架材料表面的邻苯二酚类衍生物的聚合层而负载的。此负载金属催化剂的制备方法简便，使用于多种载体骨架材料，并可负载一种或多种金属离子或纳米颗粒，具有普适性。此负载金属催化剂具有活性金属分散性好，载体比表面积大，用量少，成本低等优点。此负载贵金属催化剂是一类广泛应用的环境友好的催化剂材料，可高效用于染料废水脱色、废水有机污染物消除、汽车尾气处理、空气净化、加氢及重整催化反应、气体传感器、燃料电池等环境化工领域

摄食转“全鱼”基因黄河鲤小鼠的生理和病理分析

为了消除公众对转基因生物安全性的疑虑，加速转 “全鱼”基因黄河的产生化进程 ，本实验按类似国家卫生部瘭药毒理实实验规范进行了昆明种小鼠摄食转“全鱼”基因黄河鲤后生理和病理研究。与对照组小鼠比较，两者在生长、等方面，没有显著性差异，摄食转基因鱼对小鼠的生殖机能及对子一代幼仔的生长和发育也无影响。实验结果，转“全鱼”基因黄河鲤鱼与普通黄河鲤鱼在食品安全方面具有实质等同性

摄食转“全鱼”基因黄河鲤小鼠的生理和病理分析

为了消除公众对转基因生物安全性的疑虑 ,加速转“全鱼”基因黄河鲤(CyprinuscarpioL Huanghevar )的产业化进程 ,本实验按类似国家卫生部新药毒理学实验规范进行了昆明种小鼠摄食转“全鱼”基因黄河鲤后生理和病理研究。与对照组小鼠比较 ,两者在生长、血液常规、血生化成分、组织病理等方面 ,没有显著性差异(P >0 0 1) ;摄食转基因鱼对小鼠的生殖机能及对子一代幼仔的生长和发育也无影响。实验结果显示 :转“全鱼”基因黄河鲤鱼与普通黄河鲤鱼在食品安全方面具实质等同

Electrochemical Performance of Si-C Composites Prepared by Discharge-Plasma Assisted Milling

首次采用介质阻挡放电等离子体辅助高能两次球磨制得Si-C复合材料，其结构为微纳尺度硅颗粒均匀分散于微米级碳基体上. Si-C复合电极首周期循环放电容量为1259 mAh·g-1，20和100周期循环的容量分别为474和396 mAh·g-1. 该电极充放电曲线和交流阻抗测试的结果表明，复合材料中的硅和碳均参与锂离子嵌/脱反应，且其电荷传导阻抗明显低于纯Si.Silicon-carbon (Si-C) composites, with microstructure of multi-scaled Si particles being homogenously dispersed in micro-sized carbon matrix, had been prepared by dielectric barrier discharge plasma assisted two-step milling for the first time. The Si-C composite anode had a discharge capacity of 1259 mAh·g-1 at the first cycle, while the capacity retained 474 and 396 mAh·g-1 after 20 and 100 cycles, respectively. Charge-discharge curves and AC impedance response indicated that both silicon and carbon phases in the composite anode were involved during the lithiation/delithiation reactions and the electron transport resistance in the Si-C composite anode was much lower than that in the pure Si anode.国家自然科学基金项目（No. 51201065, No. 51231003）、中央高校基本科研业务费专项资金（No. 2012ZM0001）和广东省自然科学基金博士科研启动项目（No. S2012040008050）资助作者联系地址：华南理工大学 材料科学与工程学院，先进储能材料广东省重点实验室，广东 广州 510641Author's Address: School of Materials Science and Engineering, South China University of Technology，Guangdong Key Lab of Advanced Materials for Energy Storage, Guangzhou 510640, China通讯作者E-mail：[email protected]

(40(2):209-224)Regional Trial on the Yield Performance and Adaptability of Hybrid Rice Strains

試驗的目的在調整雜種水稻品系之種植適期，利用疑似感光性，探討每年種植單期作條件下，是否可發揮雜種優勢而提高增產潛力，以應稻田轉作政策及改進輪作制度之需。本試驗於民國77年利用氣溫較高之夏秋季於全省七個地點進行，測驗八個秈稻雜種品系之稻穀產量及適應性。試驗結果顯示；參試品系在各地之稻穀產量差異極大，在桃園新屋、花蓮、宜蘭三星三處因種植期較早，臺秈雜育1、7及11號等三品系因抽穗障礙而致產量偏低或全無收穫，僅有臺秈雜育2與6號兩品系的平均稻穀產量比對照品種臺中秈10號稍高；在彰化大村等四處採用一般正常二期作之早植，疑似感光性之臺秈雜育1、7及11號三品系平均稻穀產量較對照品種增產10.1─24.9％。臺秈雜育2號在各試驗地區產量最穩定，不論於五月的中間作或七月的一般二期作早植栽培，其平均產量都超過對照品種臺中秈10號。試驗結果顯示如能適當的調整種植時期及利用雜種品系之疑似感光性，可使雜種水稻品系發揮其高產潛力。Experiments were conducted at 7 locations to study the yield performance, adaptability and response to photoperiod of 8 indica hybrid rice strains, Rice was cultured as a mid-season crop with transplanting dates ranged from May to July in 1988. Experimental results indicated significant yield variation for hybrid strains among locations. At Hsinwu (Taoyuan), Sanhsing (Ilan) and Hualien, early transplanting in May rendered the three photosensitive strains, i. e. Tainung Sen Hybrid (TNSH) Nos. 1,7 and 11, to show difficulties in panicle extrusion. Grain yield was therefore very low, or even no harvest, for these strains. For overall average grain yield of the other 5 photo-insensitive strains, only TNSH Nos. 2 and 6 was higher than the check variety Taichung Sen No. 10. At Tatsun (Changhua), Lutsao (Chiayi), Pingtung and Taitung, late transplanting in June and July was carried out and different results were recorded. The three photo-sensitive strains out yielded the check variety by 10.1-24.9%, and all the photo-insensitive strains. TNSH No.2 showed the best adaptability as its grain yield averaged from 7 locations was 6.3% higher than the check variety. Based on the results of this experiment, it is suggested that by adjusting planting date and utilizing photosensitivity, yield performance of hybrid rice can be improved greatly when cultured under local conditions