Effects of Root Salt Stress on Growth and Allocation of Mineral Elements in Halophyte and Glycophyte Seedlings

为了解盐胁迫对植物的影响,研究了根系nA Cl胁迫在温室条件下对盐生植物榄仁(TErMInAlIA CATAPPA)和甜土植物枇杷(ErIObOTryA JAPOnICA)幼苗生长、矿质元素和灰分含量的影响。结果表明:在根系盐胁迫下,两种植物幼苗的叶片病斑多分布于中心区,灰分含量增加,幼苗的nA+-Cl–呈极显著的正相关关系,盐胁迫对两种植物幼苗的5种矿质元素(CA2+,Mg2+,nA+,k+,Cl–)含量影响不大,但它们在植物中的分布发生了变化。可见,盐生植物和甜土植物抗盐性的区别是量上的不同,没有质的差别。In order to understand the effect of root salt stress on seedlings, the growth, mineral elements and ash contents in halophyte Terminalia catappa and glycophyte Eriobotrya japonica seedlings were studied in greenhouse.The results showed that the spots mainly distributed on center of two seedling leaves under root salt stress, and ash content increased.There was significant positive relationship between contents of Na+ and Cl– after root salt stress.The contents of mineral elements(Ca2+, Mg2+, Na+, K+, Cl–) in seedlings varied little, but their distribution in seedlings happened to change.So, there is quantitive difference between halophytes and glycophyte under salt stress, and no qualitative difference.林业科技支撑计划项目(2009BADA2B0605); 福建省科技重点项目(2013N01010365); 漳州市科技重点项目(ZZ2014036)资

激光等离子体质谱用于岩样化学成份的分析

在自制的仪器上,记录了一系列岩样的激光等离子体质谱。通过对正负离子质谱的分析,可以确定岩样的化学组成。本文通过对仪器工作原理、实验条件和测试结果的介绍,显示出该测试手段具有分析速度快、分析结果准确可靠、所得信息较为丰富、可进行微区与深度分析等优点。文章还讨论了不足之处及改进方向

Mechanism of Surface Formation for Two-dimensional Ultrasonic Vibration Assisted Grinding of Monocrystal Silicon with Vertical Workpiece Vibration

通过单晶硅磨削试验以及单颗金刚石磨粒划擦试验,分析垂直于工件平面的二维椭圆超声振动磨削单晶硅的表面形成机制。椭圆超声振子由压电陶瓷晶体与金属弹性体粘接制成,其伸缩模态和弯曲模态频率相同,当输入具有一定相位差的两个交流电压信号时产生二维椭圆振动。试验结果表明,由于二维椭圆振动的施加改变了单晶硅的材料去除机制,加工表面质量明显提高,表面粗糙度显著降低,磨削沟槽变浅而宽,切屑变厚而短,单晶硅材料延性域去除比例增加;通过改变超声振动振幅与磨削深度之间大小关系,可实现磨削刃对工件的连续性接触去除和断续性接触去除两种模式的转变。The mechanism of surface formation for two-dimensional elliptic ultrasonic vibration assisted grinding with the vertical direction to workpiece is investigated by grinding experiments and single point diamond abrasive scratching experiments on monocrystal silicon.The vibrator is produced by bonding a PZT on a metal elastic body,frequencies of both longitudinal mode and bending mode are nearly the same,and an elliptic motion occurs when two alternating current signals with phase difference are applied to the PZT.The experimental results show that the workpiece surface quality is improved obviously,and the surface roughness is decreased significantly.And moreover,the grinding grooves become much shallower and wider,and the chip is also much thicker and shorter.It is known that the percent of ductile mode removal material increases.In addition,as the relation between vibration amplitude and grinding depth of cut is changed,two kinds of material removal mechanism,continuous contact and interrupting cutting,can be realized.国防科研资助项目(62301090103

Autophagy Inhibition by Chloroquine Sensitizes Cervical Cancer SiHa Cells to CPT Treatment

自噬诱导是肿瘤细胞对化疗药物抵抗性的原因之一,该研究探讨溶酶体抑制剂氯喹对喜树碱(camptothecin,CPT)诱导的宫颈癌细胞Si Ha死亡的增敏效果。CPT和/或氯喹处理宫颈癌Si Ha细胞,MTT法检测细胞增殖,DAPI和TUNEL染色观察细胞凋亡,Western blot和免疫荧光检测自噬及凋亡相关蛋白。结果发现,CPT处理后,Si Ha细胞MAP1LC3B荧光点和LC3II(microtubuleassociated protein light chain 3II)蛋白水平增加,p62荧光点和蛋白质水平则减少;而采用氯喹特异抑制自噬后,可明显提高CPT诱导的细胞凋亡、caspase-9的激活和PARP(poly ADP-ribose polymerase)的切割,而全长caspase-2水平显著下降。以上结果提示,氯喹可通过抑制细胞自噬而增强宫颈癌细胞株Si Ha对CPT诱导细胞凋亡的敏感性。The autophagy induction is one of the reasons for the resistance of tumor cells to chemotherapy drugs. In this study, the enhanced sensitivity of cervical cancer Si Ha cells to camptothecin(CPT)-induced cell death by chloroquine(a lysosome inhibitor) was investigated. The cell viability was detected by MTT assay, meanwhile, apoptosis was observed by DAPI and TUNEL, autophagy related proteins and apoptosis proteins were analyzed by immunofluorescence(IF) staining and Western blot in Si Ha cells after CPT treatment alone or combined with chloroquine. The results found that in Si Ha cells with CPT treatment the autophagy related protein LC3 foci and microtubule-associated protein light chain 3II(LC3II) protein level was increased, but p62 foci and protein level was decreased. When autophagy was inhibited by chloroquine, the CPT-induced apoptosis was obviously enhanced, and caspase-9 was activated and PARP was cleaved, but full length caspase-2 was decreased. Taken together, these results indicated that the inhibition of autophagy by chloroquine could sensitize cervical cancer Si Ha cells to CPT inducing cell apoptosis.山西省自然科学基金(批准号:2014021037-9);; 山西医科大学汾阳学院博士启动基金(批准号:1301);山西医科大学汾阳学院科研项目基金(批准号:1422)资助的课题~

庚型肝炎病毒基因在大肠杆菌中表达的初步研究

利用原核表达载体 pRSET或 (和 )pGEX在大肠杆菌内表达了覆盖庚型肝炎病毒 (HGV)C NS3和NS5区的多段基因。CE1、E2、NS3、NS5及NS3 NS5嵌合基因等的 8段基因均有高效表达 ,各重组蛋白产量与菌体总蛋白之比在 10 %～ 35 %之间。对以上重组蛋白进行免疫学筛选 ,证实其中 7个重组蛋白均具免疫学活性 ,在一定程度上确定了重组HGV抗原表位的分布 ,为HGV的血清学和免疫学诊断试剂的研究奠定了坚实基

Self-Cleaning Glass Antireflection Films Prepared by Sol-Gel Method

在酸催化体系中以正硅酸乙酯(TEOS)和纳米锐钛矿型二氧化钛粉末为SIO2-TIO2复合溶胶原料,采用提拉法在高硼硅玻璃基片表面制备出具有增透和自清洁功能的薄膜。研究了制备工艺对薄膜增透和光致超亲水性能的影响。制备的样品在400~800 nM波段,光线垂直入射时,透射率增加3%,紫外光照射一定时间后,样品对水接触角不超过5°,具有光致超亲水作用。The self-cleaning glass antireflection film was prepared on the surface of high borosilicate glass substrate by using TEOS and nanometer anatase type of titanium dioxide powder as sol-gel SiO2-TiO2 composite raw materials and using Czochralski method.The effect of preparing technique on antireflection and photoinduced super hydrophilicity properties was investigated.The experiments show that the light transmittance of prepared sample increased about 3% at wavelength between 400nm and 800nm under light vertical incidence.After UV irradiation for a certain time,the water contact angle was ≤5° which exhibited the characteristic of photoinduced super hydrophilicity.福建省区域科技重大项目(2011H41010083

动脉粥样硬化性肾动脉狭窄的早期发现

探讨冠状动脉造影同时行肾动脉造影的必要性及动脉粥样硬化性肾动脉狭窄的相关危险因素分析。共 4 91例临床疑似冠心病患者行冠状动脉造影同时行非选择性或选择性肾动脉造影 ,并对相关的临床因素进行评价 ,筛选出动脉粥样硬化性肾动脉狭窄的独立危险因素。冠心病组患者中动脉粥样硬化性肾动脉狭窄的发病率为2 0 % ,显著高于非冠心病组 (2 .6 % )。冠心病、外周血管疾病是动脉粥样硬化性肾动脉狭窄的独立危险因素 (多元Logistic回归分析示 ,P <0 .0 0 1、P =0 .0 0 3) ,是其早期发现的指标。对怀疑有冠心病的患者 ,在冠状动脉造影同时行肾动脉造影有助于动脉粥样硬化性肾动脉狭窄的早期发现

激光等离子体源飞行时间质谱计

本文介绍了自行研制的一台激光等离子体源飞行时间质谱计.该义器以高能量密度的脉冲激光作用于固体样品,产生的高温等离子体由原位的飞行时间质谱计作质谱分析.该装置具有能同时记录正负离子质谱,智能化程度高、质量分辨好等优点.而且具有较强的研究能力,既能用于原子簇离子的激光产生与质谱研究,又可以对各种无机和有机样品进行结构研究与分析测式

Development of a Two-dimensional Ultrasonic Vibration Assisted Grinding Technique of Monocrystal Silicon

基于超声振动磨削能有效提高加工效率及加工表面质量的特性,通过设计具有伸缩和弯曲两种模态的压电陶瓷椭圆振子,实现了单晶硅二维椭圆振动磨削技术。对超声振子的振动特性进行检测,证实改变压电陶瓷两电极之间的交流信号相位差和电压幅值,可得到不同形状和振幅的椭圆振动。对单晶硅进行超声磨削与普通磨削的对比试验,结果表明二维振动磨削的磨削力大幅降低,表面粗糙度显著减小,表面质量明显提高,加工表面延性域去除比例增加。从而证实二维超声振动磨削方法能够实现高效率高质量单晶硅加工。Based on the characteristics of higher machining efficiency and higher surface quality of ultrasonic vibration grinding,a two-dimensional ultrasonic vibration grinding of monocrystal silicon technique is achieved by designing elliptical vibrator with longitudinal mode and bending mode.The measurement results on the vibration characteristics of vibrator show that shape and amplitude of elliptical vibration can be modulated by changing voltage amplitude and phase difference between the piezoelectric ceramics electrodes.The grinding experimental results show that under the elliptic ultrasonic vibration assistance,grinding forces are reduced largely,the surface roughness is decreased significantly,the surface quality is improved obviously,and moreover the percentage of ductile-mode removal material increases.These indicate that high efficiency and high surface quality machining of monocrystal silicon can be achieved with elliptic vibration assisted grinding.国防科研资助项目(62301090103