改良分子信标-双重实时荧光PCR快速检测SARS病毒

目的建立改良分子信标-双重实时荧光PCR检测SARS病毒的方法,用于SARS的早期诊断和动物溯源。方法利用改良分子信标技术、装甲RNA和双片段双色荧光技术,根据GenBank公布的SARS病毒聚合酶基因1b的阅读开放框架结构的保守序列,自行设计一对引物和探针,以部分临床标本的酶联吸附实验结果和传统细胞培养方法作为对照,建立分子信标检测SARS病毒的方法。对368份临床标本(咽漱液、血液、粪便、尿液)、52份细胞培养液和50份动物标本进行荧光PCR扩增。结果分子信标检测SARS病毒的方法灵敏度为10～100个拷贝ml,与流感病毒等呼吸道病毒无交叉反应。分子信标检测368份临床标本,20份阳性。其中确诊病例阳性率为21.27%(1047),确诊病例的咽漱液阳性率为43.48%,还分别从粪便和血清中检测到SARS病毒。52份细胞培养液,29份阳性,阳性率为55.77%。50份动物标本,23份阳性,阳性率为46%。结论改良分子信标-双重实时荧光PCR检测SARS病毒方法灵敏度高、特异性强,可用于SARS的临床早期诊断和动物溯源

Operation log based synchronization algorithm for cloud storage service with multiple clients

传统的基于状态的数据同步算法具有数据传输量大、每次都需要重新开始等缺点,不能满足实际应用需求,提出了一种基于操作日志的云存储数据同步算法.通过在服务器端记录用户的操作日志,对比操作日志生成同步操作序列,回放操作序列的方法实现了高效的数据同步.与传统算法相比,该算法具有数据传输量小,快速高效,对云端服务器负载小,支持双向和增量同步等优点.算法支持同步过程中的失效恢复,适用于带宽有限和网络不稳定等极端场景.The traditional data synchronization algorithm is based on the file status. Although it is simple to understand and implement, it does not support incremental update and lacks flexibility. So it does not meet the user's requirements. An operation logs based on synchronization algorithm is proposed, which records operation logs both at the server side and at the client side, and generates synchronization operation sequences by merging these logs. In contrast with the traditional method, the log based algorithm is more effective and has fewer loads to the cloud-side server. What is more, this algorithm supports failure recover, which is especially suitable for applying to the limited bandwidth and network instability scenario

Fabrications of Three Copper Sulfide Counter Electrodes and Their Influences on Photovoltaic Properties in QDSSCs

近几年,量子点敏化太阳能电池因其具有低成本、易合成、高的光电转换效率等优点而广受关注.半导体金属硫化物具有良好的物理和化学性质,被广泛应用于各个领域,其中,铜硫化物凭借其优异的电化学催化活性,而成为量子点敏化太阳能电池良好的对电极材料.本文通过3种不同的方法在FTO表面生长Cu S纳米阵列(依次记为Cu S-1、Cu S-2、Cu S-3),并对样品进行晶相表征、表面形貌分析、电化学性能测试以及相应量子点敏化太阳能电池器件组装,最终发现Cu S-3样品具有最优的光电性能.Quantum dot-sensitized solar cells(QDSSCs) have attracted intensive attention in scientific and industrial fields due to their high molar extinction coefficient, spectral tunability by particle size, ease of fabrication, and low cost. In the past years,semiconductor metal sulfides have attracted extensive attention because of their attractive physical and chemical properties for potential applications in many fields,such as Pb S, Cu S, Co S and Cd S. In particular, copper sulfides have become a promising candidate for counter electrode materials in QDSSCs for their outstanding electrochemical and catalytic properties. In order to explore more stable and efficient copper sulfide counter electrode materials, in this work, we used three different methods to synthesize copper sulfide nanosheet arrays(marked as Cu S-1, Cu S-2, Cu S-3), which were then characterized by XRD, SEM and electrochemical workstation. XRD patterns showed that all the three samples were copper sulfide(Cu:S = 1:1). And SEM images revealed that the fabrication methods of Cu S significantly affected their morphologies. The obtained Cu S-1, Cu S-2 and Cu S-3 nanosheet arrays exhibited enhanced PCEs up to 2.92%,2.58% and 3.27%, respectively, when used as CEs in QDSSCs, implying increases of 87%,65% and 109% as compared to Pt-based QDSSCs, respectively. Through all the characterizations, we found that the Cu S-3 showed the best catalytic activity in the reduction of polysulfide electrolyte among the three samples.国家自然科学基金(No.21503177);; 中央高校基本科研业务费(No.20720150031);; 高等学校学科创新引智计划(“111计划”;No.B16029)资

北方农牧交错带引种抗逆植物筛选及经济适用性评估——以怀来为例

以初步筛选的63种抗逆性花草植物为试材,在北方农牧交错带的典型地区张家口市怀来县进行引种。通过观察植物生长状况,筛选出适应该区生长的优良花草品种,运用层次分析法对花草植物进行适应性评价,再根据引种植物的经济可行性,评估抗逆花草在园林绿化中的应用价值。结果表明,与乡土植物相比,外来植物的越冬保存率降低了约27个百分点;乡土植物种植方式不会影响植物越冬,但对外来植物有一定的影响,且分株种植都比种子种植的成活率高;在引种的63种植物中,有18种植物的环境适应性良好和经济适用性较高,可以大规模引种,分别为五彩石竹、拂子茅、燕麦、狼尾草、羊草、毛茛、扁蓿豆、鼠尾草、蓝花鸢尾、荷兰菊、地被菊、三七景天、八宝景天、德国景天、天人菊、黄花鸢尾、萱草、福禄考,其中有德国景天、金娃娃萱草、荷兰菊、燕麦有矮壮化现象,此研究对北方农牧交错带草地恢复、资源配置及区域绿化有积极的作用

A Novel Flexible Dye-Sensitized Solar Cell Based on Pt Networks Counter Electrode

柔性染料敏化太阳能电池(DSSCs)作为一种新型的化学太阳能电池,因其精简的封装工艺、较低廉的价格、高的化学稳定性以及可弯折等优点而备受关注.本文介绍了一种新型的柔性DSSC的制备,其光阳极为高度有序的氧化锌(Zn O)纳米线阵列,对电极为柔性、导电、透明的网状铂(Pt networks)电极.相对于传统的铂对电极而言,这种Pt networks对电极不仅具有优异的导电能力,还展现了极好的透光性(方阻~100Ω·sq-1,~80%透光率)和催化性能,此外,Pt networks电极可构筑于任意弯曲的衬底,具有优异的机械耐弯折性能.在Zn O纳米线阵列的DSSCs的应用中,基于Pt networks膜的柔性DSSC的转化效率比铂纳米丝阵列(Pt nanofiber arrays,Pt NFs)膜高出了32%.Flexible dye-sensitized solar cells(DSSCs) have received widespread attentions in recent years because of their ease of fabrication, low production cost, relatively high chemical stability and flexibility. We report the fabrication of Zn O DSSCs based on the highly flexible, conducting, catalytic and transparent Pt networks counter electrode, and the performance of this solar cell was characterized by SEM, XRD, polarized fluorescence microscope, motor, I-V test station and electrochemical workstation. Compared to Pt nanofiber arrays(NFs), Pt networks not only show a better conductivity, but also exhibit superior transparency and catalytic activity. Furthermore, Pt networks electrode could be constructed on arbitrary flexible substrates. The fabricated flexible Zn O DSSC based on Pt networks counter electrode achieved 1.33% photoelectric conversion efficiency, which is 32% higher than that based on Pt NFs.国家自然科学基金项目(No.51502253);; 中央高校基本科研业务费(No.20720150030);; 高等学校学科创新引智计划“111计划”(No.B16029)资

Fabrications of three copper sulfide counter electrodes and their influences on photovoltaic properties in QDSSCs

近几年，量子点敏化太阳能电池因其具有低成本、易合成、高的光电转换效率等优点而广受关注. 半导体金属硫化物具有良好的物理和化学性质，被广泛应用于各个领域，其中，铜硫化物凭借其优异的电化学催化活性，而成为量子点敏化太阳能电池良好的对电极材料. 本文通过3种不同的方法在FTO表面生长CuS纳米阵列（依次记为CuS-1、CuS-2、CuS-3），并对样品进行晶相表征、表面形貌分析、电化学性能测试以及相应量子点敏化太阳能电池器件组装，最终发现CuS-3样品具有最优的光电性能.Quantum dot-sensitized solar cells (QDSSCs) have attracted intensive attention in scientific and industrial fields due to their high molar extinction coefficient, spectral tunability by particle size, ease of fabrication, and low cost. In the past years, semiconductor metal sulfides have attracted extensive attention because of their attractive physical and chemical properties for potential applications in many fields，such as PbS, CuS, CoS and CdS. In particular, copper sulfides have become a promising candidate for counter electrode materials in QDSSCs for their outstanding electrochemical and catalytic properties. In order to explore more stable and efficient copper sulfide counter electrode materials, in this work, we used three different methods to synthesize copper sulfide nanosheet arrays (marked as CuS-1, CuS-2, CuS-3), which were then characterized by XRD, SEM and electrochemical workstation. XRD patterns showed that all the three samples were copper sulfide (Cu:S = 1:1). And SEM images revealed that the fabrication methods of CuS significantly affected their morphologies. . The obtained CuS-1, CuS-2 and CuS-3 nanosheet arrays exhibited enhanced PCEs up to 2.92%，2.58% and 3.27%, respectively, when used as CEs in QDSSCs, implying increases of 87%，65% and 109% as compared to Pt-based QDSSCs, respectively. Through all the characterizations, we found that the CuS-3 showed the best catalytic activity in the reduction of polysulfide electrolyte among the three samples.国家自然科学基金（Nos.21503177），中央高校基本科研业务费（NO.20720150031）、高等学校学科创新引智计划（“111 计划”，B16029）资助作者联系地址：1. 生物仿生与软物质研究院，福建省柔性功能材料重点实验室，物理系，物理科学与技术学院，厦门大学，厦门361005；2. 固体表面物理化学国家重点实验室，化学系，化学化工学院，厦门大学，厦门361005；3. 纤维材料改性国家重点实验室，材料科学与工程学院，东华大学，上海201620Author's Address: 1. Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Lab for Soft Functional Materials Research, Department of Physics, College of Physical Science and Technology, Xiamen University, Xiamen 361005; 2. State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemistry Engineering, Xiamen University, Xiamen 361005; 3. The State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, P.R. China.通讯作者E-mail：[email protected]

A Novel Flexible Dye-Sensitized Solar Cell Based on Pt Networks Counter Electrode

柔性染料敏化太阳能电池（DSSCs）作为一种新型的化学太阳能电池，因其精简的封装工艺、较低廉的价格、高的化学稳定性以及可弯折等优点而备受关注. 本文介绍了一种新型的柔性DSSC的制备，其光阳极为高度有序的氧化锌（ZnO）纳米线阵列，对电极为柔性、导电、透明的网状铂(Pt networks)电极. 相对于传统的铂对电极而言，这种Pt networks对电极不仅具有优异的导电能力，还展现了极好的透光性（方阻~ 100 Ω•sq-1，~80%透光率）和催化性能，此外，Pt networks电极可构筑于任意弯曲的衬底，具有优异的机械耐弯折性能. 在ZnO纳米线阵列的DSSCs的应用中，基于Pt networks膜的柔性DSSC的转化效率比铂纳米丝阵列 (Pt nanofiber arrays, Pt NFs）膜高出了32%.Flexible dye-sensitized solar cells (DSSCs) have received widespread attentions in recent years because of their ease of fabrication, low production cost, relatively high chemical stability and flexibility. We report the fabrication of ZnO DSSCs based on the highly flexible, conducting, catalytic and transparent Pt networks counter electrode, and the performance of this solar cell was characterized by SEM, XRD, polarized fluorescence microscope, motor, I-V test station and electrochemical workstation. Compared to Pt nanofiber arrays (NFs), Pt networks not only show a better conductivity, but also exhibit superior transparency and catalytic activity. Furthermore, Pt networks electrode could be constructed on arbitrary flexible substrates. The fabricated flexible ZnO DSSC based on Pt networks counter electrode achieved 1.33% photoelectric conversion efficiency, which is 32% higher than that based on Pt NFs.国家自然科学基金项目（51502253）、中央高校基本科研业务费（20720150030）及高等学校学科创新引智计划（“111 计划”，B16029）资助作者联系地址：1. 生物仿生与软物质研究院，福建省柔性功能材料重点实验室，物理系，物理科学与技术学院，厦门大学，厦门361005；2. 固体表面物理化学国家重点实验室，化学系，化学化工学院，厦门大学，厦门361005Author's Address: Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Lab for Soft Functional Materials Research, Department of Physics, College of Physical Science and Technology, Xiamen University, Xiamen 361005; 2. State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemistry Engineering, Xiamen University, Xiamen 361005通讯作者E-mail：wxguo@xmu. edu. c

基于电子转移/高能碰撞解离质谱碎裂的O-GlcNAc糖基化位点定量分析方法在高脂喂养小鼠肝脏糖蛋白质组分析中的应用

O-连接N-乙酰葡糖胺翻译后修饰(O-GlcNAc)是发生在蛋白质丝氨酸或者苏氨酸位点上的单糖修饰,由于其具有化学计量数少、在质谱鉴定中离子化效率低以及无特定的氨基酸序列等特点,使O-GlcNAc翻译后修饰位点的定性和定量分析难度较大。本研究结合基于凝集素弱亲和色谱的O-GlcNAc修饰糖肽富集方法、本研究组发展的准等重六重标记定量方法和电子转移/高能碰撞解离(EThcD)模式的高分辨质谱技术,发展了一种O-GlcNAc修饰位点的高通量定量分析方法,对高脂喂养小鼠肝脏中蛋白质O-GlcNAc修饰位点进行规模化定量分析。共定量分析了783个O-GlcNAc位点,其中122个位点的表达量存在明显差异,对应于85个O-GlcNAc蛋白,并初步探讨了脂代谢过程中O-GlcNAc修饰的作用。本研究有望为肥胖和胆固醇酯沉积等营养代谢相关疾病提供新的治疗思路