Effect of Salinity Stress on the Activities of ATPase and Phosphatase in the Mud Crab(Scylla serrata)

采用酶学分析的方法研究了盐度(分别为5、15、25、35)胁迫下锯缘青蟹鳃、肌肉和肝胰腺中腺苷三磷酸酶和磷酸酶活性变化。结果表明,随着盐度降低,青蟹鳃中Na+,K+-ATPase、Ca2+,Mg2+-ATPase活性均升高,随着胁迫时间延长,Na+,K+-ATPase活性呈现不同的变化规律,且趋于平缓。青蟹肌肉中ACP活性随着盐度的升高而呈小幅度下降,而肝胰腺中ACP活性则呈小幅度升高,但各组之间差异不显著(P>0.05)。不同胁迫时间同一盐度组青蟹肌肉中的ACP活性有升有降。青蟹肌肉、肝胰腺中AKP活性随着盐度升高而升高;随着胁迫时间延长,同一盐度组青蟹肌肉中AKP活性均呈升高趋势,肝胰腺中AKP活性有升有降。由此可见,盐度对青蟹生理生化影响显著。The mud crab(Scylla serrata) is a kind of important marine culture species in China.Salinity is one of the most important environmental factors which relates closely with the development of S.serrata.The activities of ATPase and phosphatase in gill,muscle and hepatopancreas of S.serrata were determined under different salinities(5,15,25,35) for understanding its physiological-biochemical response to salinity stress.The results showed that the effects of salinity stress on Na+,K+-ATPase activity in the gill of S.serrata were significant(P0.05).The AKP activities in both muscle and hepatopancreas increased with the salinity increasing,and increased with the time duration in the same treatment.Both ACP and AKP activities in hepatopancreas were higher than those in muscle.The changes of AKP activities in hepatopancreas and muscle seemed to be related with Na+,K+-ATPase activities changing in gill under different salinities.These results suggested that the physiological-biochemical response of S.serrata to salinity stress was significant,which could offer some theoretic basis for water quality management of mud crab aquaculture.厦门大学高层次人才引进科研启动项目“锯缘青蟹营养需求及其免疫营养研究”;; 江苏省滩涂生物资源与环境保护重点建设实验室项目(JLCBE05007

Effect of salinity stress on the activities of phenoloxidase and superoxide dismutase of the serum、tissue and organ of mud crab,Scylla serrata

采用酶学方法测定了盐度(5、15、25、35)胁迫下锯缘青蟹血清中酚氧化酶(PO)及其肌肉和肝胰腺中超氧化物歧化酶(SOD)活性的变化.结果表明,盐度胁迫显著影响青蟹血清PO活性(p<0.05).S5、S15低盐度处理组青蟹血清中PO活性显著高于S25、S35高盐度处理组(p<0.05),同一处理组青蟹血清中PO活性除S15处理组随胁迫时间延长而持续下降外,其余各组呈现出有升有降的变化.各处理组在盐度低于或高于25时,青蟹肌肉中SOD活性升高,且胁迫至72h前,随着胁迫时间延长,SOD活性下降;青蟹肝胰腺中SOD活性随着盐度升高而降低,且同一处理组青蟹肝胰腺中SOD活性则随着胁迫时间的延长而呈现出先降后升的趋势.这表明环境盐度胁迫对青蟹体内SOD活性影响显著,且表现出明显的时间效应性.由此可见,盐度胁迫显著影响青蟹的PO、SOD活性,进而影响青蟹免疫力.This paper studied the effect of salinity(5,15,25 and 35) stress on the biochemical response of Scylla serrata by enzyme analysising.Scylla serrata were acclimated in close-cycle system before transferred to different groups.Each treatment was run in triplicate.The activities of phenoloxidase(PO) and superoxide dismutase(SOD) were determined after 24~96h.The results showed that the phenoloxidase activity was increased significantly with salinity decreasing in salinty(p<0.05).The serum PO activities of S5 and S15 were significantly higher than those of S25 and S35(p<0.05).Each experiment group displayed certain changes except that the S15 group showed the trend of incessantly descending.Meanwhile,the SOD activity of muscle in mud crab increased significantly when transferred to 5,15 and 35(p<0.05).The effect of the muscle SOD activity of Scylla serrata in the experimental group decreased with the time over 72h stress.Whereas the SOD activity in hepatopancreas of mud crabs that transferred from salinity 25 to salinity 5,15 and 35 increased.The influence of salinity stress on the SOD activity of mud crab was significant(p<0.05),relative to the elapsed time.In conclusion,the effects of salinity stress on the non-specific immunity of mud crab would affect activities of PO in serum and SOD in muscle and the hepatopancreas.国家“863”计划项目(2007AA091406);; 宁波市自然科学基金项目(2006A610086);; 江苏省滩涂生物资源与环境保护重点建设实验室项目(JLCBE05007);; 厦门大学985计划科技创新项目等部分资