Studies on Rice Resistance-related Proteins in Response to Bacterial Leaf Streak,Xanthomonas Oryzae pv.Oryzicola

水稻细菌性条斑病由XAnTHOM OnAS OryzAEPV.OryzICOlA引起,是目前威胁亚洲稻区水稻生产的重要病害之一。以水稻品种9311为研究对象,通过差异蛋白质组学方法,研究了病菌侵染48 H后水稻叶片的差异表达蛋白质,通过分析比对、质谱分析以及数据库检索,从中选择了7个上调表达的鉴定蛋白,包括4个水稻lrk类基因,2个nbS-lrr类基因和1个Pr-10基因家族成员基因。并设计相应的PCr引物,从水稻CdnA中获得相关基因片段做探针进行nOrTHErn杂交分析,结果显示,上述基因在接种病原菌12 H或48 H后表达量增加,表明这些蛋白质参与了抗病反应。Rice bacterial leaf streak(BLS) caused by the pathogen Xanthomonas oryzae pv.oryzicola(Xooc) is one of the major diseases in Asia.In this paper,the protein differently expressed after infected for 48 h by BLS was studied with rice 9311 as material through proteomic approach.Based on the result of protein blast,fingerprints and data search analysis,seven identified proteins up-regulated were selected including four LRK like genes,two NBS-LRR type genes and one PR-10 gene.Relevant primers were designed to amplify the genes from rice cDNA as probe for Northern blotting.The results showed that the mRNA levels of these genes increased significantly after infected by BLS for 12 h and 48 h,indicating these proteins have participated in disease resistance.国家863计划项目(2007AA10Z132);国家重大科技专项(2008ZX08001-001;2009ZX08009-045B);教育部重点项目(重点01102)资

表面等离子体调制单模面发射激光器的研究

对单模面发射半导体激光器的研究随着其应用的不断扩展而引起了人们的广泛的重视,应用多种方法可以提高其输出功率并改善其模式抑制比.利用金属表面等离子体纳米结构调制的方法可以获得单模面发射激光器输出功率的提高,理论计算表明这种方法增强效应可达近50%

利用纳米压印技术制备面发射表面等离子体激光器的方法

一种利用纳米压印技术制备面发射表面等离子体激光器的方法，其特征在于，该方法包括如下步骤：步骤1：在激光器的出光面上制作一层金属薄膜；步骤2：在金属薄膜上涂敷一层液态阻蚀剂；步骤3：把压印模板上的纳米图案压在液态阻蚀剂上；步骤4：采用压印技术，使液态阻蚀剂固化；步骤5：脱模，把纳米图案转移到阻蚀剂上；步骤6：等离子体刻蚀多余阻蚀剂，在金属薄膜表面形成由阻蚀剂构成的纳米图案；步骤7：反应离子刻蚀，把纳米图案转移到金属薄膜上；步骤8：去除剩余的阻蚀剂，完成制备

异育银鲫粘孢子虫病的季节动态及药物治疗

目的通过对人工养殖异育银鲫体表和鳃部寄生的两种粘孢子虫包囊的季节动态研究,了解粘孢子虫病的流行规律;并通过药物治疗试验,探讨控制粘孢子虫病的有效途径。材料与方法寄生于异育银鲫鲤部的银鲫碘泡虫和寄生于体表的武汉单极虫的季节动态调查,以及武汉单极虫病的药物治疗试验,都在湖北省鄂州市附近的一个渔场中进行。调查日期分别是1999年8月至2000年7月和1999年6月至11月,每次取鱼30尾以上;在解剖镜下进行粘孢子虫包囊的检查和记

中红外下半导体掺杂调制的表面等离子体透射增强效应

理论研究了平面电磁波通过n型重掺GaAs薄膜的透射谱.当GaAs薄膜两表面刻上亚波长的周期性沟槽结构时,透射谱在中红外波段出现了异常的透射增强现象.把这一现象归因于表面等离子体模式和波导模式的耦合.通过优化结构参数可以得到最大的透射效率.此外,发现随着掺杂浓度的升高,透射谱线中的透射峰逐渐向高频方向移动,最优化后透射峰值随掺杂浓度的升高而逐渐降低.这是由于掺杂浓度的改变,导致了不同的等离子体频率和电子碰撞频率,从而影响了激发模式和薄膜对电磁波的吸收

Study on MS2 Mediated Virus-like Particles Vaccine Against Foot-and-month Disease

研究表明,口蹄疫病毒(fOOT-And-MOuTH dISEASE VIruS,fMdV)的VP1蛋白含有关键的抗原决定簇.根据O/Hl-JOC12/03猪源fMdV毒株VP1上第141~160位氨基酸序列设计引物,利用重叠延伸PCr(SOE PCr)技术将该序列插入在大肠杆菌(ESCHErICHIA COlI)噬菌体MS2外壳蛋白基因的特定位点.然后连接到原核表达载体PET28A上,构建了重组表达质粒28A-CP-VP1,转化bl21(dE3),ITPg诱导表达得到融合表达的CP-VP1.透射电子显微镜下观察融合蛋白CP-VP1成类病毒颗粒(VIruS-lIkE PArTIClES,VlPS)状.间接ElISA实验证实该蛋白能够特异地结合豚鼠抗O型fM-dV的阳性血清,30μg的CP-VP1的VlPS蛋白免疫小鼠后可以诱导其产生高效价的特异抗体.故该口蹄疫病毒抗原决定簇展示在噬菌体MS2上表面的VlPS蛋白具有开发成口蹄疫疫苗的前景.Foot-and-mouth disease(FMD) has caused severe economic losses to millions of farmers in many countries all over the world.The severity of the disease and the spread of this epidemic virus have not been controlled fruitfully,and it continues to be a threat to livestock.Vaccination is one of the most effective weapons against the infectious disease.Our study was aimed to develop a kind of effective and safe virus-like particles(VLPs) vaccine.VP1 protein of foot-and-mouth disease virus(FMDV) has key antigenic determinants which were used by vaccine developers time and again.The nucleotides coding the peptides from 141 to 160 AA of VP1 protein of strain HLJOC12/03 FMDV were amplified and inserted into the specific site in the coat protein gene of MS2 by using SOE PCR.The products were digested by enzymes and then ligated to the pET28a.The recombination plasmids were transformed into Escherichia coli BL21(DE3) host cell,expressed with IPTG induction,and the high level expression of the protein were harvested.The recombinant CP-VP1 virus-like particles(VLPs) protein was observed by transmission electron microscopy.A series of immunogenicity tests were carried out and the experimental data proved that the VLPs protein we had designed showed fine effects and it would be a kind of hopeful vaccine against FMD,and this kind of VLPs can also be applied to detect FMD.福建省科技厅重点项目(2009N0051); 福建省自然科学基金项目(2010J01240); 厦门市科技计划项目(3502Z20103007

Distribution characteristic of clay minerals contents in Minjiang River and its environmental significance

为探究闽江下游及河口区粘土矿物的含量分布特征,于2009年在该区域采集表层沉积物样品25个,利用X射线衍射进行了粘土矿物的含量测定.结果表明粘土矿物的相对百分含量范围分别为:高岭石(33%~47%,平均值为42%),伊利石(27%~43%,平均值为34%),绿泥石(20%~29%,平均值为24%),蒙脱石(0%~3%,平均值为1%).在显著水平为0.01时,伊利石与高岭石、绿泥石的含量呈显著负相关关系,伊利石与高岭石的相关系数为-0.825,伊利石与绿泥石的相关系数为-0.611.通过对闽江下游及河口区粘土矿物含量分布的分析及与中国近海的粘土矿物含量分布对比,闽江下游及河口区粘土矿物的含量分布受物质来源、地形条件、介质环境、粘土矿物自身颗粒大小形态和人类活动的影响.相对于黄河和长江,闽江表层沉积物中具有较高的高岭石含量,较低的伊利石含量,与珠江的粘土矿物组合特征类似,这反映出气候效应的影响.In order to explore the distribution of clay minerals contents in Minjiang River,25 surface sediment samples were collected in 2009.In this paper,clay mineral content of the Minjiang River was measured with X-ray diffraction.The result showed that clay minerals were composed of kaolinite( 42%),illite( 34%),chlorite( 24%)and montmorillonite( 1%).There are good negative correlations of illite with kaolinite and with chlorite.At the significant level of 0.01,the correlation coefficient between illite and kaolinite is- 0.825,and the correlation coefficient between illite and chlorite is- 0.611.The terrigenous kaolinite and illite at sites near the estuary are determined and distributions of clay minerals contents between the Minjiang River and offshores in China compared.In addition to the matter source influence,the distribution of clay minerals contents in Minjiang River are affected by matter source,terrain conditions,depositional environment,particle size and shape of clay minerals and the anthropogenic impacts.In contrast to the Yellow River and the Yangtze River,Minjiang River,similar to that of the Pearl River,has higher content of kaolinite and lower content of illite which suggested the evident of climatic impacts.国家自然科学基金资助项目(41076122;40976037;41376050