独活寄生汤调控对大鼠椎间盘软骨细胞Wnt/β-catenin信号通路的影响

目的:从Wnt/β-catenin信号通路探讨独活寄生汤水提物对大鼠椎间盘退变软骨细胞功能的影响。方法:（1）用水提加热回流法制备独活寄生汤水提物成分;（2）选取4周龄健康雄性SD大鼠30只,采用机械-酶消化法分离大鼠椎间盘软骨组织,建立软骨细胞体外培养体系并进行鉴定;（3）RT-PCR、We stern blot法分别检测经DKK-1抑制剂干预及（或）经白细胞介素-1β诱导的椎间盘软骨细胞Wnt4、GSK-3β、β-catenin mRNA与蛋白含量的表达。结果:（1）椎间盘软骨细胞经Ⅱ型胶原法染色后,阳性对照组胞浆区域浸染为棕黄色;（2）RT-PCR、Western blot检测结果示,与正常组比较,模型组Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达显著升高（P<0.01）;与模型组比较,独活寄生汤水提物组(100,200,400μg·mL-1)的Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达显著降低（P<0.01或P<0.05）,其中以200μg·mL-1组的表达量最低（P<0.01）。结论:独活寄生汤水提物组可通过调控Wnt/β-catenin信号通路,下调大鼠椎间盘退变关节软骨中Wnt4、GSK-3β、β-catenin mRNA与蛋白含量表达,从而延缓椎间盘软骨细胞退变。福建省自然科学基金(2017J01224);;福建中医药大学校管课题(X2015034

Diatom diet selectivity by early post-larval abalone Haliotis diversicolor supertexta under hatchery conditions

Benthic diatoms constitute the primary diet of abalone during their early stages of development.To evaluate the dietary preferences of early post-larval abalone,Haliotis diversicolor supertexta,we analyzed the gut contents of post-larvae that settled on diatom films.We compared the abundance and species diversity of diatom assemblages in the gut to those of the epiphytic diatom assemblages on the attachment films,and identified 40 benthic diatom species in the gut contents of post-larvae 12 to 24 d after settlement.The most abundant taxa in the gut contents were Navicula spp.,Amphora copulate,and Amphora coffeaeformis.Navicula spp.accounted for 64.0% of the cell density.In the attachment films,we identified 110 diatom species belonging to 38 genera.Pennate diatoms were the dominant members including the species Amphiprora alata,Cocconeis placentula var.euglypta,Cylindrotheca closterium,Navicula sp.2,and A.coffeaeformis.Nano-diatoms(<20 μm in length) accounted for a considerable proportion of the total species..

一种高效氨合成催化剂及其制备

一种高效氨合成催化剂，由载体和活性成分组成，其特征在于：载体为活性炭材料；以钌为主活性成分，担载量为０．１～１６％ｗｔ，以碱土金属、稀土金属、碱合金为助剂，钌与助剂摩尔比为０．０１～１０。其制备方法是先用含钌化合物溶液浸渍载体；在２００～５００℃氢气氛下还原３０分钟～５小时；再浸渍碱土、稀土或碱金属溶液作助剂。本发明具有在较低温度和压力下将氮和氢转化为氨的能力。带填

堇青石蜂窝陶瓷载体上SAPO-34分子筛的原位合成

运用水热合成技术在孔密度为６２ｃｅｌｌｓ／ｃｍ＾２的堇青石蜂窝陶瓷上原位合成了ＳＡＰＯ－３４分子筛，并用ＸＲＤ和ＳＥＭ等技术对其进行了表征，结果表明，经过一次水热合成，堇青石蜂窝陶瓷载体的表面上可牢固均匀地生长一层厚度约为３０μｍ的ＳＡＰＯ－３４分子筛，而相应的载体增重约为１５％－２５％，改变合成条件还可使堇青石载体上同时生长ＳＡＰＯ－３４和ＳＡＰＯ－５分子筛。此外，对ＳＡＰＯ－３４分子筛在堇青石载体上的生长机理也作了初步探讨