不完全性脊髓损伤患者主要照顾者负性情绪与创伤后成长的纵向研究

目的探讨不完全性脊髓损伤患者主要照顾者在其治疗不同时期负性情绪与创伤后成长的纵向变化及二者之间的关系。方法以不完全性脊髓损伤患者主要照顾者76例作为研究对象,采用心境和焦虑症状问卷、创伤后成长量表在术前、术后进行问卷调查。结果心境和焦虑症状总分术后三个月(50.34±3.46)与术前(57.23±9.06)、术后一周(56.89±9.03)相比有显著差异,术后一个月心境和焦虑症状总分(52.30±9.05)与术后一周(56.89±9.03)相比有显著差异,差异有统计学意义(P<0.05);术后三个月创伤后成长总分(62.43±11.03)与术前(56.72±12.75)、术后一周(58.71±14.63)相比有显著差异,术后一个月创伤后成长总分(60.12±14.48)与术后一周(58.71±14.63)相比有显著差异,差异有统计学意义(P<0.05)。结论负性情绪各维度与创伤后成长相关。负性情绪各维度与创伤后成长总分、新的可能性、个人力量、自我转变相关

藏药镰形棘豆的化学成分、药理作用及毒理学研究进展

目的:为进一步研究和开发利用藏药镰形棘豆提供参考。方法:以"镰形棘豆""莪达夏""化学成分""药理作用""毒理学""Oxytropis""Oxytropis falcata""Oxytropis falcata Bunge""Chemical constituents""Chemical composition""Pharmacologic actions""Toxicology"等为关键词,组合查询2010年1月-2016年2月在Pub Med、中国知网、万方、维普等数据库中的相关文献,对镰形棘豆的化学成分、药理作用、毒理学研究及临床应用进行综述。结果与结论:共检索到相关文献81篇,其中有效文献46篇。目前从镰形棘豆中分离鉴定的化学成分主要包括黄酮类、生物碱类、甾体及萜类等化合物,其具有抗炎镇痛、抗脓毒症、抗氧化、防紫外线损伤、抗缺血缺氧性损伤、抗肿瘤、免疫调节、抗纤维化、促凝止血、抑菌、止咳祛痰等广泛的药理作用;毒性研究报道并不多见;镰形棘豆除在藏医中常被用来治疗疫疠、麻风、感冒、便血、骨伤、刀伤等之外,现已有一些以镰形棘豆为主要组成的上市药品和医院制剂应用于临床。今后应加强其药理、药效、作用机制和毒理学研究。原南京军区医学科技创新项目(No.14MS085

Analysis on the characteristics of macrobenthis community in the North-west Daya Bay of South China Bay in spring

利用2008年春季在大亚湾西北部海域开展的12个测站的大型底栖动物调查资料,对该海域大型底栖动物的群落特征进行分析。结果表明该海域内大型底栖动物优势种由粗帝汶蛤(TIMOClEA SCAbrA)、波纹巴非蛤(PAPHIA undulATA)、上野蜾蠃蜚(COrOPHIuM uEnOI)、方背鳞虫(lEPIdOnOTuS SQuAMATuS)、刀明樱蛤(MOErEllA CulTEr)和角海蛹(OPHElIA ACuMInATA)等6种组成;可划分为4个群落,群落Ⅱ和群落Ⅰ为主体群落,群落Ⅲ和群落Ⅳ分布区较小,群落间差异较大。大型底栖动物数量上以个体较大的软体动物占优,但群落的组成则以多毛类和甲壳类为主,其组成情况基本上反映了群落的特征。群落Ⅰ以多毛类-甲壳类为表征,群落Ⅱ则以甲壳类-多毛类-软体动物为表征。栖息环境的差异,是造成群落间差异的主要原因;群落多样性水平呈群落Ⅲ>群落Ⅱ>群落Ⅰ>群落Ⅳ,沉积环境的污染状况和人为扰动是影响多样性水平的主要因素;群落整体的稳定性较好,未受到明显的扰动,但分布于航道区域和渔业生产作业区的群落Ⅳ和群落Ⅲ,在航道建设、疏浚和渔业生产的影响下,群落受到一定程度的扰动,稳定性较弱。Based on the data collected from 12 research stations in the North-west Daya Bay,South China Sea in spring 2008,the characteristics of macrobenthis community were examined.The results showed that the dominant macrobenthic species were Timoclea scabra,Paphia undulate,Corophium uenoi,Lepidonotus squamatus,Moerella culter and Ophelia acuminate.The macrobenthos community could be divided into four groups with notable differences.Group Ⅰ and Ⅱ were the dominant groups,while the distribution areas of Groups Ⅲ and Ⅳ were relatively small.Overall,molluscs was dominant in quantity in the macrobenthos community but the community was mainly composed of Annelida and Arthropoda,reflecting the characteristics of the community.The dominant macrobenthic species of group Ⅰ were Timoclea scabra,Nephtys oligobranchia,Theora lata,Sigambra hanaokai,Glycinde gurjanvae,Prionospio malmgreni,Timoclea scabra,Turritella bacillum,Corophium tridentium,Phyllodocidae und.,Nectoneanthes alatopalpis,Ericthonius pugnax,Pseudopythina sp.and Stylochus ijimai.Species belonging to this group distributed in the nearshore area of Aotou Harbor and Nanhai Petrochemicals Basement Group Ⅱ assemblage includes Paphia undulata,Timoclea scabra,Corophium uenoi,Prionospio malmgreni,Sigambra hanaokai,Ophelia acuminata,Mediomastus sp.,Nephtys oligobranchia,Moerella culter,Corophium lamellatum,Paraprionospio pinnata and Prionospio pulchra.These species distributed mainly around the Chunzhou Island where suspended long line culture of oyster was common.The discriminating species for Group Ⅰ were S.hanaokai,N.soligobranchia,P.malmgreni,N.alatopalpis and E.pugnax while group Ⅱ were C.uenoi,P.malmgreni,S.hanaokai,N.oligobranchia,T.scabra,M.sp.,Phyllodocidae und.,P.pinnata,O.acuminata,C.lamellatum,M.culter and Chaetozone sp.Group Ⅲ assemblage were mainly found in the west side of Mabianzhou,where it was a fishing ground for Arthropoda in Daya Bay.Group Ⅳ was distributed in area where main shipping routes passed through,in the east side of area of Group Ⅱ and in between the area of Group Ⅰ and Ⅲ.For each community group,while group Ⅱ Group Ⅰ was dominated by Annelida and Arthropoda while Group Ⅱ was dominated by arthropoda,annelida and mollusca;such difference is mainly because of the difference in habitat conditions.Diversity of macrobentho community was highest in Group Ⅲ,followed by Group Ⅱ,Group Ⅰ and then Group Ⅳ.Poor habitat condition caused by pollution,sedimentation and other human disturbances were the main reasons for the differences in diversity between groups.In general,macrobenthos community in the North-west Daya Bay of South China Sea was stable in spring.However,community structure of Groups Ⅳ and Ⅲ,which distributed in the main navigation route and fishing grounds,were less stable;suggesting the disturbances from maintenance of navigation channel and fishing activities might have reduced community stability.中央级公益性科研院所基本科研业务费专项基金(2007ZD08;2007TS08;2010YD10

Role of eNOS / NO signaling pathway in peritubular capillary lesions in renal interstitial fibrosis and the related mechanism in mouse models of unilateral ureteral obstruction

目的探讨内皮型一氧化氮合酶(EndOTHElIAl nITrIC OXIdE SynTHASE,E nOS)和一氧化氮(nITrIC OXIdE,nO)在单侧输尿管梗阻(unIlATErAl urETErAl ObSTruCTIOn,uuO)肾间质纤维化小鼠微血管病变中的作用及机制。方法64只kM小鼠随机分为两组:假手术组n=32只;单侧输尿管梗阻uuO组n=32只。观察4周,每周检测各组小鼠血bun、SCr及一氧化氮,流式细胞计数外周血Cd133+/VEgfr+内皮祖细胞(EndOTHElIAl PrOgEnITOr CEllS,EPCS)、MASSOn染色观察肾组织形态学变化,免疫组化法检测肾间质Cd34+表达计数微血管密度,实时定量PCr检测肾皮质E nOS、VEgf MrnA表达。结果 uuO组血一氧化氮、内皮祖细胞计数、肾间质微血管密度、E nOS、VEgf MrnA表达水平持续下降,在第2、3、4周与对照组差异有统计学意义。一氧化氮水平与肾间质微血管密度呈正相关(r=0.715,P<0.05);E nOS MrnA表达水平与肾间质微血管密度(r=0.624,P<0.05)、内皮祖细胞计数(r=0.375,P<0.05)、VEgf MrnA(r=0.351,P<0.05)呈正相关。结论 E nOS/nO途径参与了uuO小鼠肾间质微血管的调节,其调节涉及对血管舒张功能影响、介导促血管肾脏因子VEgf MrnA表达及动员内皮祖细胞等机制。Objective To investigate the role of eN OS/NO signaling pathway in peritubular capillary lesions of mouse renal interstitial fibrosis with unilateral ureteral obstruction( UUC) and the potential mechanism.Methods Sixtyfour healthy male KM mice were randomly divided into sham operated group( n = 32) and unilateral ureteral obstruction group( n = 32).At each week,serum BUN,Scr and NO were determined and the percentages of CD133+/ VEGFR+endothelial progenitor cells in peripheral blood mononuclear cells were detected by flow cytometry.Morphological changes of the renal tissue were observed using Masson staining.The expression of CD34+cells in the renal interstitium was analyzed by immunohistochemistry to calculate the peritubular capillary density.The expressions of eN os and VEGF mRNA were determined by real-time PCR.Results The expression of blood NO,the percentages of endothelial progenitor cells,peritu-bular capillaries,eN OS mRNA,and VEGF mRNA in the UUO group were significantly decreased compared with those of the sham group at 2,3,and 4 weeks( P < 0.05).NO was positively correlated with peritubular capillaries( r = 0.715,P< 0.05),eN OS mRNA was positively correlated with peritubular capillaries( r = 0.624,P < 0.05),endothelial progenitor cells( r = 0.375,P < 0.05),and VEGF mRNA( r = 0.351,P < 0.05).Conclusions eN OS / NO signaling pathway participates in regulation of peritubular capillary lesions in renal interstitial fibrosis of UUO mice.The mechanism may be partly related to the regulation of vasomotor reflex,the expression of VEGF mRNA and mobilization of endothelial progenitor cells.福建省卫生厅青年科研课题(编号:2010-2-92

PHOTOSYNTHETIC CHARACTERISTICS of PHYTOPLANKTON IN THE DAYA BAY

利用PHyTO-PAM测量大亚湾浮游植物的光合作用能力、非化学淬灭并建立快速光曲线,同时研究浮游植物群落结构、组成、丰度和相应的环境因素,分析光合作用特征与浮游植物生长和分布的耦合关系。本次调查期间大亚湾浮游植物以集群化硅藻为优势物种,各站位最大光量子收益达到0.72。大亚湾浮游植物适应高光强,当光照达到1000μE/(M2.S)时电子传递速率逐渐达到饱和;光照达到1500μE/(M2.S)浮游植物依然可进行光合作用,叶黄素循环保护光合器官使其免受高光强损伤;当光照达到1700μE/(M2.S)时,光合器官可能受到损伤,此时电子传递速率下降,实际光量子收益接近于0。集群化硅藻能够耐受较广的光照范围及其活跃的光合特征有利于它们在全球海区的广泛分布。Photosynthetic capacity,rapid light curves and NPQ of phytoplankton in the Daya Bay were measured using pulse-amplitude-modulation(PAM) fluorometry.Phytoplankton dynamics and environmental parameters were also investigated with the purpose of assessing the coupling between photosynthetic capacity and phytoplankton distribution.During the investigation periods,multi-cellular diatoms were dominant,and their maximum quantum yields were up to 0.72.Our results revealed that acclimation of phytoplankton in the Daya Bay to high irradiance allowed saturated electron transport to occur at PAR of 1000μmol/(m2.s).The development of non-photochemical quenching and xanthophylls cycle activity could protect the photosynthesis apparatus once PAR was elevated to 1500μmol/(m2.s).The PAR of 1700μmol/(m2.s) triggered biophysical alterations in photosynthesis apparatus,resulting in the declined effective quantum yield and electron transport rate.The accumulation to extensive temperature range and high photosynthetic activity are in favor of the worldwide distribution of multi-cellular diatoms in the natural environments.国家重点基础研究发展计划(973)项目;2011CB403603号;国家自然科学基金项目;41106117

Subspace quantum process tomography via nuclear magnetic resonance

基于三核自旋量子系统实现了内嵌两量子位子空间量子过程重构.通过输入完备态集合对执行的量子过程进行了表征.由于量子过程内嵌于子空间,使得重构实验所需输入次数及时间显著减少,同时实验尽量避免使用时间较长的J偶合演化,有效控制了系统的退相干。Experimental investigation of subspace quantum process tomography in three-spin system was implemented via nuclear magnetic resonance.A quantum process was characterized by measuring a complete set of input states and corresponding outputs.The method using ancillary qubit remarkably reduces the number of the initial input states.And the pulse sequences used in this paper have fewer J-coupling evolutions.The experiment time was shortened and quantum decoherence of the system was weakened efficiently.福建省自然科学基金(批准号:2008J0219)资助的课题---

Comparative study of the DNA damage in three tissues of blue mussel(Mytilus edulis) after exposure to three typical POPs

采用彗星电泳技术,比较研究了Aroclor 1254、BaP、DDT三种典型POPs对紫贻贝(Mytilus edulis)鳃、性腺、消化腺细胞的DNA损伤效应的差异,以期为进一步评价这三种典型POPs遗传毒理机制及其预警监测提供科学依据。结果表明,Tail DNA%是评价DNA损伤的理想指标,另外,在急性毒性试验中,这三种POPs对紫贻贝鳃、性腺、消化腺细胞的DNA损伤作用均存在显著时间-剂量-效应关系,其中,BaP的DNA损伤作用最大,鳃和消化腺细胞对毒性作用较敏感。同时,对不同组织细胞的敏感性差异原因及其在环境监测中的应用进行了探讨。

三种典型POPs对紫贻贝不同组织DNA损伤的比较研究

采用彗星电泳技术,比较研究了Aroclor 1254、BaP、DDT三种典型POPs对紫贻贝(Mytilus edulis)鳃、性腺、消化腺细胞的DNA损伤效应的差异,以期为进一步评价这三种典型POPs遗传毒理机制及其预警监测提供科学依据。结果表明,Tail DNA%是评价DNA损伤的理想指标,另外,在急性毒性试验中,这三种POPs对紫贻贝鳃、性腺、消化腺细胞的DNA损伤作用均存在显著时间-剂量-效应关系,其中,BaP的DNA损伤作用最大,鳃和消化腺细胞对毒性作用较敏感。同时,对不同组织细胞的敏感性差异原因及其在环境监测中的应用进行了探讨

Vertical distribution of zooplankton in the continental slope southwest of Nansha Islands,South China Sea

浮游动物是海洋生态系统物质和能量转移的关键环节,也是渔业资源的重要饵料,其种类组成和数量分布均有明显的垂直分层现象。利用2011年4月在南沙群岛西南大陆斜坡海域开展的18个站点90份样品的调查数据,对该海域浮游动物的垂直分布进行了研究,表明:(1)该海域浮游动物种类组成丰富,共出现18个类群580种(类);(2)浮游动物种类组成垂直变化明显,特定水层出现的种类数占总种数的43.6%,各水层均出现的种类仅占总种数的15.8%;(3)优势种组成复杂,垂直变化明显,单一种类的优势度不高;(4)浮游动物平均密度和湿重生物量分别为206.27Ind/M3、94.03Mg/M3,密度和生物量均以0~2M层和30~75M层较高,沿水深梯度的变化呈明显的双峰型;(5)浮游动物数量的垂直变化主要受温跃层影响,温跃层内浮游动物数量最高,温跃层上方和下方的水层内数量较低;(6)南沙西南大陆斜坡区浮游动物生产力水平较高,表明该海域渔业资源有一定的开发潜力。As dietary organisms of fish stocks,zooplankton is a key link of mass and energy transformation in the marine ecosystem.There are vertical changes in the species compositions and abundances of zooplankton in the water column.Based on 90samples from 18stations collected in April 2011,the vertical distribution of zooplankton in the continental slope to the southwest of Nansha Islands,South China Sea was studied.It was found that the continental slope waters are rich in zooplankton species,with a total number of 580species belonging to 18taxa being recorded.There are obvious vertical variations in the zooplankton species compositions.The numbers of zooplankton species occurring in a specific deep group could take up 43.6%of the total number of species,while the number of species occurring in all deep groups made up only 15.8%of the total number of species.There was no obvious dominant species and the dominancy of any single species was low.The overall mean density and wet biomass of zooplankton were 206.27ind/m3 and 94.03mg/m3 respectively.The higher density and biomass were found in the deep groups of 0—2mand 30—75mand the vertical distribution showed a pattern of double-humped curve.Thermocline was an important factor in the vertical distribution of zooplankton.The abundances of zooplankton in the thermocline layer were higher than those in the upper mixed layer and the water layers bellow the thermocline.Compared to the other regions in the South China Sea,zooplankton abundances were the highest in the continental slope southwest off Nansha Islands,suggesting potentials for fishery development in the region.中央级科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2012TS02); 农业部财政重大专项(NFZX2013); 国家科技支撑计划课题(2013BAD13B06