对虾养殖水体中一株降解氨氮芽孢杆菌的分离鉴定及其发酵配方的优化

本实验在（28±1）℃下从对虾养殖池塘中分离筛选具有氨氮（包括游离氨NH3、铵盐NH+4）降解效果的芽孢杆菌（Bacillus）,并采用单因素实验对筛选出的芽孢杆菌进行发酵配方优化,以此挑选出低廉高产的发酵培养基配方。菌株分离筛选及氨氮降解实验结果显示:经初步分离,得到5株芽孢杆菌（DS1、DS2、DS3、DS4、DS5）,再通过氨氮降解实验得到一株能够显著降解水体氨氮的芽孢杆菌DS5,5 d内菌株DS5对水体氨氮降解迅速,在第4天达到最大降解率（36.41±0.07）%,且最后3 d水体氨氮持续稳定在36%左右;通过菌株16S rDNA序列分析及生理生化鉴定,将菌株DS5（GenBank登录号:MK629979）初步鉴定为枯草芽孢杆菌（Bacillus subtilis）。菌株DS5发酵配方实验结果表明:在28℃下,当碳源为5‰葡萄糖、氮源为10‰豆粕、无机盐为5‰氯化钠时,菌株生长良好,培养基含菌数较高,综合考虑成本、菌含量等因素,选择5‰葡萄糖-10‰豆粕-5‰氯化钠作为该菌的最优发酵培养基配方

凡纳滨对虾养殖亲本群体遗传多样性分析

运用微卫星标记技术,选用8对微卫星引物对厦门市5个凡纳滨对虾亲虾群体(ZA1、HN、ZA2、ZP、DS)进行遗传多样性分析。结果显示,8对微卫星引物在5个凡纳滨对虾亲虾群体中共检测到75个等位基因,各群体的平均等位基因数(Na)为3. 875～7. 000、平均有效等位基因数(Ne)为2. 632～3. 719、平均PIC值范围在0. 498～0. 624。平均观测杂合度(Ho)为0. 410～0. 562、平均期望杂合度(He)为0. 589～0. 687。各群体的平均近交系数(Fis)为0. 147～0. 305,说明各群体内近交程度较高,存在杂合子缺失现象。除ZP与DS群体外,其他群体在多个微卫星位点上均显著偏离平衡(P <0. 05),说明大部分群体存在杂合子缺失现象。遗传变异分析结果显示,各群体间的遗传分化指数(Fst)为0. 028～0. 199;根据Fst计算得到各群体间的Slatkin’s遗传距离在0. 029～0. 249之间。本研究结果表明,采自厦门市的5个凡纳滨对虾亲虾群体均存在不同程度的近交现象。本文通过对厦门市5个凡纳滨对虾亲虾群体进行遗传多样性分析,旨在明确厦门市凡纳滨对虾亲虾群体的遗传结构、种质资源状况及近交程度,为该地区凡纳滨对虾种质资源的提纯、保优、复壮及遗传选育提供背景资料和建议。国家虾产业技术体系项目(CARS-48);;福建省科技厅重大专项专题项目(No:2016NZ0001-4);;厦门海洋经济创新发展示范项目(16CZY009SF05

Effect of Kangquan Recipe on Apoptosis Regulatory Genes bax and bcl-2mRNA in Prostate of Rats

目的探讨康泉方对实验性大鼠前列腺组织凋亡调控基因bax mRNA、bcl-2 mRNA的影响。方法采用大鼠去势后注射丙酸睾丸酮致前列腺增生法造模,同时灌胃给药30天后,处死大鼠取前列腺组织并测量湿重,RT-PCR法检测各组前列腺腹叶组织bax mRNA、bcl-2 mRNA的表达。结果与模型组比较,康泉各剂量组前列腺湿重显著下降(P0.05);与模型组比较,康泉各剂量组bax mRNA及bax/bcl-2显著升高(P0.05)。结论康泉方对大鼠良性前列腺增生有明显的治疗作用,其作用机理可能通过调节前列腺组织bax mRNA、bcl-2 mRNA表达,促进前列腺细胞凋亡,并呈明显量效关系。Objective To investigate the effect of Kangquan Recipe (KQR) on apoptosis regulatory genes bax and bcl-2 mRNA in prostate of rats. Methods Benign prostatic hyperplasia model rat was established by injecting testosterone after castration. The model rats were killed and prostate glands were removed for examination after being treated with administration of KQR by gastrogavage for 30 days. The wet weight of prostate was measured and the mRNA expressions of bax and bcl-2 in rats' tissue of abdominal lobe of prostate were determined by RT-PCR. Results Compared with the model group, wet weight of prostate was lower significantly in the groups treated with different dosages of KQR (P0.05). Compared with the model group, the expressions of bax mRNA and ratios of bax/bcl-2 were significantly higher and the expressions of bcl-2 mRNA significantly lower in the KQR treated groups (P0.05). Conclusion KQR shows an obvious treatment effect on rats with benign prostatic hyperplasia, the mechanism might be through effectively regulating the expressions of bax mRNA and bcl-2 mRNA in prostatic tissue to accelerate the cell apoptosis of prostate in obvious dose-effect manner.福建省中医药科研项目(No.wzy0616);; 厦门市医学科研项目(No.WSK0526);; 厦门市卫生青年创新课题(No.WQK0510

Effect of Kangquan Recipe on Basic Fibroblast Growth Factor of Prostate Tissue in Rats

目的探讨补肾通络的康泉方对实验性大鼠前列腺组织碱性成纤维细胞生长因子(basic fibro-blast growth factor,bFGF)表达的影响。方法采用大鼠去势后注射丙酸睾丸酮致前列腺增生法造模,同时灌胃中药康泉方30天后,取大鼠前列腺组织,采用免疫组化和逆转录聚合酶链式反应(RT-PCR)法分别检测各组前列腺腹叶组织bFGF表达。结果与模型组比较,康泉高、中剂量组bFGF和bFGFmRNA表达明显降低(P0.05)。结论康泉方能够降低实验性良性前列腺增生大鼠前列腺组织bFGF表达。Objective To investigate the effect of Kangquan Recipe(KQR)on basic fibroblast growth factor(bFGF)in prostatic tissue of rats.Methods Benign prostatic hyperplasia model rat was established by injecting testosterone after castration.After being administered with KQR by gastrogavage for 30 days,the model rats were killed and their abdominal lobe prostate glands were taken for determining the protein and mRNA expressions of bFGF using immunohistochemical method and RT-polymerase chain reaction(RT-PCR)respectively.Results The expressions of bFGF and bFGF mRNA were significantly lower in the model rats being treated with high or medium dose of KQR than those in the untreated model rats(P0.05).Conclusion KQR can effectively decrease the expression of bFGF in prostatic tissue of experimental benign prostate hyperplasia model rats.福建省中医药科研项目(No.wzy0616);; 厦门市医学科研项目(No.WSK0526);; 厦门市卫生青年创新课题(No.WQK0510