The Design and Implementation of Fixed Assets Information Management System Based on RFID

固定资产是企业资产的一个重要组成部分。由于固定资产在使用中具有价值高、使用周期长、使用环境差别大、存放地点分散、使用流程复杂等特点，造成了在目前的企业固定资产管理过程中存在着许多的问题。比如：管理部门和实物管理部门之间信息无法联系，造成账面信息和实物信息不一致；有些企业拥有数量庞大的固定资产，却没有集成的信息系统，大多采用资产设备卡片或纸质条形码标签，通过传统的手工方式管理，缺乏有价值的固定资产管理信息，固定资产的管理困难，工作效率低；在实际工作中不容易做到账、卡、物的一一对应，资产盘点费时、费力，效果有限，且差错率高。这些问题给固定资产的管理工作带来了一定的难度。 RFID射频识别技术是一...Fixed assets is an important part of enterprise assets.Because the fixed assets with high value, long use period, the use of environmental difference is big, storage locations scattered, the use of complex process in use, resulting in the management of fixed assets in the current business process that exist in many problems. Such as: Information can not contact between management and material mana...学位：工程硕士院系专业：信息科学与技术学院_计算机技术学号：X200922103

Design of Dual Processor Embedded System Based on FPGA

随着信息处理技术的不断发展，传统的嵌入式单核系统在提升整体性能上面临瓶颈，基于FPAG的多核系统设计也逐渐发展起来。基于摩尔定律，处理器芯片厂商一直在不断提高芯片工作频率来提高处理器的性能，但随着芯片设计与制造工艺的不断改进，处理器芯片上集成的晶体管已超过上亿个，在体系结构的限制下已很难单纯通过提高主频来提升性能，同时，主频提高带来的功耗问题也不容小觑。然而从应用需求来看，日益复杂的多媒体，科学计算，虚拟现实等多个领域都需要计算机提供更强大的计算能力，因此多线程，多内核技术的发展成为必然趋势。 本文综述了多核处理器架构与并行计算的发展历程，分析了并行计算机系统的体系结构，根据指令流和数据流的...With the continuous development of information technology, the single-core embedded system is difficult in improving the computing ability, and the multi-core system designed based on FPAG gradually developed. Based on Moore's Law, the manufacturers which make the processor chip have been constantly improving the operating frequency to improve the performance of the processor. However, when over h...学位：工程硕士院系专业：信息科学与技术学院_控制工程学号：2322010115321

Research on Advance Pricing Arrangement and Related Issues

跨国公司的发展已成为经济全球化的重要标志，随之而来的跨国转让定价问题也大大增加了各国税务当局负担。由于转让定价的普遍性和传统转让定价方法的弊端，预约定价制的出现受到了税务当局和纳税人的普遍欢迎。我国自1998年就开始了预约定价实践，但经验、人员和制度方面的不足阻碍了实践的发展。希望通过研究能为我国的预约定价发展做出一定的贡献。除引言和结束语外，本文共分为四章。第一章对预约定价制的定义进行了界定，分析了预约定价制产生和发展的理论基础，并对预约定价协议的性质提出了自己的看法，最后分析了预约定价制的优缺点。第二章主要对美国和OECD的预约定价制度进行了详细的研究，并介绍了国外预约定价的实践，总结了预...The development of multinational companies has become an important symbol of economic globalization, along with which transfer pricing has greatly increased the tax bureaus’ burden. Because of the universality of transfer pricing and the disadvantages of traditional transfer pricing methodologies, advance pricing arrangement is welcomed by both tax bureaus and taxpayers. China has made advance pri...学位：法学硕士院系专业：法学院法律系_国际法学(含国际公法、国际私法、国际经济法)学号：20030813

病毒对海洋细菌代谢的影响及其生物地球化学效应

病毒是海洋生态系统中丰度最高的生命形式,其中超过90%属于浮游细菌(细菌和古菌)病毒,是海洋生态系统的重要参与者和海洋生物地球化学循环的重要驱动力。作为海洋浮游细菌主要的致死因子之一,病毒通过裂解宿主释放出大量的有机物和营养盐,调控宿主群落的代谢行为,进而影响生物地球化学循环。同时,伴随侵染的发生,病毒挟持宿主细胞的代谢系统完成自身的生命周期,从而改变宿主胞内的代谢途径和代谢产物。概述了病毒在个体层面和群落层面对海洋浮游细菌代谢的影响,及其对海洋元素循环的作用,评估了气候变化、环境因子对病毒调控细菌代谢的潜在影响,有助于人们对微生物参与的海洋生物地球化学循环的全面认识。国家自然科学基金优秀青年科学基金项目“海洋病毒生态学”(编号:41522603);国家自然科学基金面上项目“溶源性噬菌体对海洋细菌生理生态特性的影响”(编号:31570172)资助~

论哈尔滨工业大学计算机教育金字塔体系

我国进入了信息化时代，计算机专业得到空前的发展，但随之而来也出现了一些问题，如专业定位、 人才培养、师资队伍建设和学生的实践教学等。本文重点介绍哈尔滨工业大学（以下简称哈工大）计算机学院 在解决计算机教育问题上的一些做法，包括计算机教育金字塔体系的建立以及在该体系下的实践和取得的成果， 希望能对读者有所裨益

Genetic Diversity Analysis of Five Populations of Paphia undulata

采用形态特征和微卫星分子标记对采自菲律宾(PH)、泰国(TH)、福建云霄(YX)、海南儋州(HN)和广西北海(BH)的5个地理群体的波纹巴非蛤(Paphia undulata)进行遗传多样性分析.形态差异分析结果显示,国外群体和国内群体形态差异明显:2个国外群体(PH和TH)形态接近,聚为一支;国内支系中,YX和HN群体的形态较近,先聚为一支,而BH群体的外部形态与前两者差异较大.Hardy-Weinberg平衡检验检测到所有群体在5个位点上均出现不同程度地偏离平衡.5个群体间遗传分化指数(FST,0.000 5~0.183 1)、基因流值(Nm,2.230 46~1 066.23)以及分子方差分析(AMOVA,群体间的变异贡献率为10.21%,p<0.000 1)结果表明,群体间存在中等程度的分化.基于遗传距离的非加权组平均法(UPGMA)聚类分析显示:2个国外群体和3个国内群体分别聚为一支,再聚为一个整体;国内支系中,YX和BH群体先聚为一体,再与HN群体聚类.研究结果揭示了波纹巴非蛤较高的遗传多样性水平和中等程度的遗传分化水平,同时也暗示其现有的遗传分化程度受到人类生产活动的干扰.Genetic diversity of five populations of Paphia undulata sampled from Philippines( PH),Thailand( TH),Fujian Yunxiao( YX),Hainan Danzhou( HN) and Guangxi Beihai( BH) was studied using the morphological variation analysis and 8 micro-satellite markers.The morphological variations showed significant differences between the Chinese group and the exotic group.Within the Chinese group,the samples from YX and HN populations were rather similar in morphology,whereas BH population differed greatly from the above two populations.Meanwhile,significant departures from Hardy-Weinberg equilibrium were observed in 5 of the 8 microsatellite loci in all the 5 populations.The lines of FST( 0. 000 5 to 0. 183 1),Nm( 2. 230 46 to 1 066. 23) and AMOVA analysis( 10. 21% of genetic variation was distributed within groups with the significant value,p<0. 000 1) across all populations indicated that there was medium level of divergence among the5 P. undulata populations.The UPGMA clustering tree based on genetic distance demonstrated that the populations of PH and TH clustered into one group,and the other three Chinese populations clustered into one other group.Within the Chinese group,the HN population clustered into one group,while the populations of YX and BH clustered together,which indicated that the YX population was more closely related to the BH population.In this study,high levels of genetic diversity within populations and moderate levels of genetic differentiation among populations were presented for P. undulata.Our data also revealed that the genetic pattern of P. undulata was likely to be disturbed by human-mediated passive dispersal via aquaculture activities.These results would provide scientific basis for the conservation and reasonable utilization of natural resources of P. undulata.海洋公益性行业科研专项(201205021-2);; 国家贝类产业技术体系项目(CARS-48

三七总苷对二甲基亚硝胺致肝纤维化大鼠胶原代谢的影响

目的:考察三七总苷对二甲基亚硝胺(DMN)致肝纤维化模型大鼠胶原代谢的影响。方法:用DMN复制中度肝纤维化模型,随机分为三七总苷组、干扰素组、三七总苷配合干扰素组、阴性对照组,分别以相应的药物连续治疗8周,检测各组大鼠血清肝功能、透明质酸及肝组织羟脯氨酸(Hyp)含量,结合常规病理HE、苦味酸-天狼星红染色,评价肝组织炎症及纤维化程度。结果:中度肝纤维化大鼠模型经过连续8周的三七总苷治疗,Hyp含量明显少于模型对照组,差异显著(P<0.05),其血清学指标、肝纤维化分级等方面均出现了不同程度的改善。结论:三七总苷能够降低大鼠胶原沉积,减少DMN所致肝纤维化大鼠胶原含量

Preparation and Application of Soluble Human Squamous Cell Carcinoma Antigen Expressed by Escherichia coli

旨在建立基于大肠杆菌表达系统的高效可溶性表达人鳞状上皮细胞癌抗原(SCCAg)方法,获得具有较好活性的重组SCCAg抗原并应用于建立抗原检测方法; 。基于pGEX-6P-l载体和大肠杆菌E. coli; ER2566菌株开展重组SCCAg抗原可溶性表达纯化方法研究,评价纯化抗原活性,筛选特异性单克隆抗体,初步建立并评价SCCAg抗原检测方法。结果; 显示,pGEX-6P-l载体和E coli; ER2566菌株可用于建立较高效的可溶性表达和纯化SCCAg抗原的方法,获得了具有较高纯度和活性的重组SCCAg抗原,筛选获得特异性单克隆抗体并; 初步建立了 SCCAg管式化学发光检测方法。建立了有效的基于大肠杆菌表达系统的可溶性表达和纯化SCCAg的方法。The aims of this study are to establish a method for efficient soluble; expression of human squamous cell carcinoma antigen(SCCAg ) based on; Escherichia coli expression system and obtain the recombinant SCCAg; antigen in fine activity, then apply it in the detection method; establishment of antigen. The study on the method of soluble expression; and purification of recombinant SCCAg antigen was conducted based on; pGEX-6P-l vector and E. coli ER2566 strain. The activity of the purified; antigen was evaluated by Abbott Kit and the specific monoclonal antibody; was screened by indirect ELISA. It was proved that PGEX-6P-1 vector and; E. coli strain ER2566 could be used to establish efficient soluble; expression and purification method for recombinant SCCAg antigen.; Moreover, the recombinant SCCAg antigen was proved to be in high purity; and activity. Thus,the SCCAg detection method of chemical luminous tube; was established with the specific monoclonal antibodies. In conclusion,; an effective method for the expression and purification of SCCAg, which; is based on the E. coli expression system, is established.国家高技术研究发展计划(863计划

Development of detection kit for T cell infected with Mycobacterium tuberculosis based on fullautomatic chemiluminescence immune analyzer

目的建立基于化学发光平台的人结核感染T细胞检测方法。方法将一株γ-干扰素单抗标记在磁微粒上,另一株γ-干扰素单抗标记在吖啶酯上,然后将检测体系与已有的细胞刺激培养体系相结合。结果本研究成功建立基于化学发光平台的人结核感染T细胞检测方法。以ElISA平台检测试剂的检测结果作为参考,该方法的灵敏度为98.3%,特异性为99.2%,总体符合率达到98.8%。结论该方法具有更高的分析灵敏度(可达0.27 Pg/M l)、更宽的线性范围(1 Pg/M l~5 000 Pg/M l)、更好的重复性(批内与批间变异系数均<6.0%)及更易实现高通量检测,为临床诊断结核感染提供了有力的工具。Objective To develop the detection kit for T cell infected with Mycobacterium tuberculosis based on full-automatic chemiluminescence immune analyzer.Mehtods An anti IFN-γ Mab was coated on the surface of microparticle.Another anti IFN-γ Mab was labelled to acridinium ester.After that, the detection system was combined with the in vitro cell culture system.Results This research developed the detection kit for T cell infected with Mycobacterium tuberculosis based on full-automatic chemiluminescence immune analyzer.Compared with the testing results of the ELISA kit, the sensitivity, specificity and total matching ratio of the CLIA kit was 98.3%, 99.2% and 98.8%, respectively.Conclusion The CLIA kit has better sensitivity(0.27 pg/m L), wilder linear range(1 pg/m L ~ 5 000 pg/m L), and better repeatability(intra and inter coefficient of variation < 6.0%).It makes a high throughput detection available.It will contribute to the clinical diagnosis of Mycobacterium tuberculosis.国家高技术研究发展计划(863计划)(2011AA02A101