ICP-MS法对闽南常见食(药)用菌的微量元素分析测定

建立微波消解-电感耦合等离子体质谱(Inductively Coupled Plasma Mass Spectrometry,ICP-MS)测定食、药用菌微量元素的方法,并对闽南地区常见的6种食、药用菌的6种微量元素锌(Zn),铜(Cu),铅(Pb),砷(As),汞(Hg)和镉(Cd)进行了分析。结果表明,所建立的方法简便、快速、准确。在所测得的6种食、药用菌中,不同的微量元素含量差别较大,其中Zn含量以巴西蘑菇最高(72.35μg/g),银耳最低(26.40μg/g),Cu含量以巴西蘑菇最高(47.77μg/g),黑木耳最低(8.67μg/g),Pb、As含量则分别在0～6.43μg/g和0.13～0.85μg/g之间变动,而Hg的最高含量为4.69μg/g,Cd的最高含量为0.50μg/g,这些结果可为人们日常生活中的膳食及药用提供一定的参考依据。国家自然科学基金资助(NO.30572316

Identification and immunogenicity analysis of artificial antigen of Glycyrrhizic acid conjugated with BSA

目的制备中药甘草的活性成分甘草酸(GA)的人工抗原及抗血清,为制备GA的单克隆抗体、并建立快速检测GA的酶联免疫吸附测定(ELISA)提供技术基础。方法将GA与载体蛋白牛血清白蛋白(BSA)偶联起来制得完全抗原后,经基质辅助激光解吸飞行时间质谱鉴定其相对分子质量,用此抗原免疫BALB/c小鼠,制备抗血清,并通过间接ELISA法和竞争ELISA法检测其抗体效价和特异性。结果合成的人工抗原GA-BSA中GA与BSA的结合比约为7∶1;免疫小鼠得到特异针对GA的多抗血清,GA抗体的效价为1∶8000。结论成功地合成了GA的人工抗原,且该抗原有较好的免疫原性,可应用于建立GA的免疫分析方法。Purpose To prepare the conjugate antigen Glycyrrhizic acid-bovine serum albumin(GA-BSA) and its antiserum to produce anti-GA monoclonal antibody and to establish a fast assay for GA content by an ELISA.Methods The conjugate antigen GA-BSA was artificially coupled and identified by matrix-assisted laser absorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS).BALB/c mice were immunized with GA-BSA to prepare antiserum against GA.The antiserum titer and specificity were detected by indirect ELISA and competitive ELISA,respectively.Results The conjugating ratio of GA and carrier BSA was 7∶1.The antiserum titer was 1∶8 000 and it reacted specifically to the GA.Conclusion The successfully synthesized conjugate antigen GA-BSA implies its feasibility in the establishment of fast immunoassay for the GA content determination国家自然科学基金资助(No.30572316

Seasonal and spatial variation of phytoplankton and relationship with water-quality factors in Futian Mangroves of Shenzhen

在探讨深圳福田红树林区浮游植物群落结构及种群数量变化规律的基础上,对红树林区不同水质环境中浮游植物时空变化进行了研究,初步分析主要水质因子与浮游植物密度时空变化的相关关系。分析表明,福田红树林区浮游植物有5门28属51种(含未知9种),以硅藻门种类为主,优势种为微小小环藻(Cyclotella caspia)和诺氏海链藻(Thalassiosira nordenskildi)等,蓝藻、绿藻能发展成为优势类群,优势种为小颤藻(Oscillatoria minima)和小球藻(Chlorella vulgaris)等。浮游植物种类组成季节变化明显,空间变化显著。浮游植物密度空间变化明显,季节差异不大。各季度的浮游植物密度较高,达到106cells·L-1的级别。由于接纳生活污水、工业废水、养殖废水等陆源污水,5个站位的主要水质因子受污水排放影响明显,站位受陆源污水影响的顺序依次为:Ⅳ>Ⅱ>Ⅴ>Ⅰ>Ⅲ,污水排放进入红树林区的水域水质显著区别于完全海水水域。陆源污水输入对浮游植物密度时空变化造成显著影响,浮游植物密度与总氮浓度、盐度相关性较好,与总氮成显著负相关,与盐度成显著正相关。Phytoplankton community were studied at 5 sites in Futian Mangrove Reserves of Shenzhen from Oct. 2005 to Sep. 2006. The species composition, densities and distribution of phytoplankton, in seasonal and spatial scale, were studied. Main water-quality factors were preliminary examined for their relations to phytoplankton densities. As result a total of 51 taxa (including 1 variety and 9 unidentified taxa) belonging to 28 genera in 5 phyta were identified. It showed that diatoms, such as Cyclotella caspia and Thalassiosira nordenski?ldi dominated in 4 seasons in most sites. Among the others, species of Cyanobacteria and Chlorophyta, such as Oscillatoria minima and Chlorella vulgaris, only dominated at a few sites. In contrast to a remarkable seasonal and spatial variation of species compositions, the phytoplankton densities changes little in season though they alternated with sites in spatial. Phytoplankton density was high reaching 106 cells?L-1 in 4 seasons. Water qualities changed with seasons in 5 sites due to the influence of domestic and industrial wastewater. The sites contaminated by the sewage are in sequence of Ⅳ >Ⅱ >Ⅴ >Ⅰ >Ⅲ, the pollution made water quality quite different with that of seawater in the open sea. Correlations of phytoplankton density with total nitrogen and salinity were found. It showed that the phytoplankton density changed positively with salinity but negatively with the total nitrogen.中国近海海洋综合调查与评价项目908-01-ST06和908-02-03-0

Research Progress in Traditional Chinese Medicine Treatments for Breast Cancer

[中文文摘]乳腺癌是影响妇女身心健康的顽疾,它的发生与多种因素相关。尽管手术、化疗、放疗、内分泌治疗等已广泛应用,但仍未找到良好的治疗方法。中医药对乳腺癌的病因病机、辨证论治有自己独特的见解,在临床治疗和基础研究方面也取得了一定的进展。中医药可以贯穿乳腺癌治疗的整个过程,已经成为乳腺癌综合治疗的一部分。[英文文摘] Breast cancer,caused by many factors,is a chronic disease affecting women′s emotion and physical health.Although surgery,chemotheropy and endocrine theropy,etc.are widely used in the treatment,no good treatment has been found so far.However,traditional Chinese medicine has its unique opinions in epiology,pathogenesis and treatment according to syndrome differentiation,which hasalso made some progress in clinical care and basic researches.Traditional Chinese medicine has been being playing a very important and useful part throughout the whole process of breast cancer′s comprehensive treatment