LTE230-Based Power Wireless Private Network Applications and Implementation on Changdao

针对山东长岛特有的复杂海岛环境、交通不便、通信网络分散等问题,提出了建设LTE230系统的发展方案。通过对LTE230系统的优势特点进行分析,着重介绍了多个智能化的应用,该系统在智能电网中发挥了不可替代的作用,其良好的覆盖效果和通信能力可满足当地的应用需求。In Shandong province, targeting Changdao's unique complex island environment, traffi c inconvenience, and the dispersion issues of communication network, the paper proposed the construction LTE230 system development program. Through the analysis of the advantages of LTE230 system characteristics, the paper emphatically introduced multiple intelligent applications. The system played an irreplaceable role in smart grid, its good coverage and communication ability met the local application demand.烟台科技项目(5206051400K9

表面增强拉曼光谱:应用和发展

表面增强拉曼光谱技术(Surface-enhanced Raman spectroscopy,SERS)是一种具有超高灵敏度的指纹光谱技术,目前已广泛应用于表面科学、材料科学、生物医学、药物分析、食品安全、环境检测等领域,是一种极具潜力的痕量分析技术。本文对SERS技术及相关的针尖增强拉曼光谱(Tip-enhanced Raman spectroscopy,TERS),壳层隔绝纳米粒子增强拉曼光谱(Shell-isolated nanoparticle-enhanced Raman spectroscopy,SHINERS)技术的发展及应用进行了综合评述,并探讨了其未来的研究热点及发展方向。国家自然科学基金项目(21533006,21522508,21775127)~

Treatment of algae bloom water using ·OH based on conventional drinking water process

我国水体的富营养化日益严重,水源地出现季节性藻类爆发现象,严重威胁饮用水安全。以厦门市莲坂水厂各工艺段出水为研究对象,采用大气压强电离放电产生羟; 基自由基(·OH),分别对水源水、混凝沉淀出水、砂滤出水进行处理,处理时间为4.5 s。当注入总氧化剂浓度为1.8 mg·; L~(-1)时,高藻浓度从25.3*10~4 cells · mL~(-1)降到800 cells ·; mL~(-1);对混凝沉淀出水注入总氧化剂0.6 mg· L~(-1)、砂滤出水注入0.2 mg·; L~(-1)时,藻细胞都未检出;·OH处理后CODMn、TOC及UV254均有明显降低,砂滤出水三卤甲烷小于8; mug·L~(-1);检测的各项指标均达到国家《生活饮用水卫生标准》(GB; 5479-2006)。因此,·OH可快速有效安全地杀灭高藻,为我国高藻水源地饮用水卫生安全保障提供技术支撑。Due to the increasing eutrophication in China, harmful algae blooms; often occur in water body periodically, which poses serious threats to; drinking water safety. Using a strong ionization discharge technology at; atmosphere pressure, ·OH was used to remove algae bloom. Other; conventional process such as coagulation, sedimentation and sand; filtration were applied respectively in the Lianban Water Plant in; Xiamen (China). The total reaction time required in the three water; processes was only 4.5 s. When the total reactive oxidant (TRO); concentration injected to algae bloom water was 1.8 mg·L~(-1), the algae; content was decreased by ·OH from 25.3*10~4 cells·mL~(-1) to 800; cells·mL~(-1). When the TRO that was injected into coagulation,; sedimentation and sand filtration water achieved 0.6 mg·L~(-1) and 0.2; mg·L~(-1), respectively, the algae contents were not detected (ND) in; treated effluents. At the same time, water quality in CODMn, TOC and; UV254 were decreased and the disinfection by-products(DBPs) such as,; trihalomethanes (THMs) concentration was lower than 8 mug·L~(-1) in; treated effluents. The treated effluent, resulting from this technology,; met the requirement of the National Drinking Water Standards (GB; 5479-2006). Therefore, ·OH was effective and safe to remove freshwater; algae without further treatment being required.国家科技支撑计划项目; 国家重大科研仪器研制项目; 科技部创新人才推进计划重点领域创新团

金属纳米结构表面等离激元共振增强光电化学反应

联合多个外场协同相互作用,表面等离激元共振(SPR)效应用于调控表面反应,可以进一步提高反应效率和选择性。在本文中,我们研究了在外加光场的作用下,通过电位对金属电极费米能级的调控,实现对SPR能量的调控,从而调节SPR弛豫产生热电子和热空穴的能量,研究SPR增强化学反应的光电协同机制,调控金属纳米结构(NPs)光电化学界面反应的选择性和效率。国家自然科学基金项目(21533006,21621091)资

Aβ1-42诱导的小胶质细胞炎性反应对神经元细胞Mecp2蛋白表达的影响

目的:体外研究Aβ1-42诱导的小胶质细胞炎症反应对海马神经元细胞甲基化Cp G结合蛋白（Mecp2）表达的影响。方法:（1）用不同浓度的Aβ1-42（终浓度分别为0,5,10,20,30μmol/L）处理小胶质细胞系（N9）,24h后取上清液,ELISA检测每组N9细胞培养上清中炎症因子TNF-α、IL-1β的含量,选择合适的Aβ1-42的终浓度。（2）用Aβ1-42（终浓度为20μmol/L）及TLR4拮抗剂TAK-242（终浓度为1μg/ml）处理小胶质细胞系（N9）,分为3组:正常对照组（con组）、单纯Aβ1-42处理组(Aβ1-42组)、Aβ1-42处理后TAK-242干预组（TAK-242组）。24 h后取上清液,ELISA检测每组N9细胞培养上清中炎症因子TNF-α、IL-1β的含量。（3）实验分组同（2）,24 h后收集培养液,分别干预生长良好的海马神经元细胞系（HT-22）。24 h后,通过Western Blot、免疫荧光染色方法检测每组HT-22细胞中Mecp2蛋白的表达。结果:（1）ELISA结果显示:与con组相比,Aβ1-42处理后,N9细胞分泌的TNF-α、IL-1β的表达量随Aβ1-42浓度的增加而增加（P<0.05）。（2）ELISA结果显示:与con组相比,Aβ1-42组的TNF-α、IL-1β的表达水平明显提高（P<0.05）;TAK-242组的细胞分泌TNF-α、IL-1β水平较Aβ1-42组显著降低（P<0.05）。（3）Western Blot结果显示:与con组相比,Aβ1-42组HT-22细胞中Mecp2蛋白表达明显增加（P<0.05）;与Aβ1-42组相比,TAK-242组HT-22细胞中Mecp2蛋白表达明显减少（P<0.05）。（4）免疫荧光染色结果显示:与con组相比,Aβ1-42组HT-22细胞中Mecp2蛋白表达明显增强;而与Aβ1-42组相比,TAK-242组HT-22细胞中Mecp2蛋白表达减弱。结论:Aβ诱导的小胶质细胞发生炎性反应,导致神经元的损伤,可能是由于这种炎性反应使得神经元细胞中Mecp2蛋白的表达量增加,从而损伤神经元。国家自然科学基金(31570845);国家自然科学基金青年项目(81501207);;陕西省自然科学基础重大研究项目(2016ZDJC-16

Surface-enhanced Raman Spectroscopic Study of Gold Nanoparticles with Different Shapes

【中文文摘】使用514 5nm激光激发,第一次得到了不同形状金纳米粒子的表面增强拉曼光谱(SERS)。一般情况下,较短波长(600nm)的激发。然而,对特殊形状的自组装金纳米粒子,由于避雷针效应,即使使用绿光激发也可获得很高增强的SERS。 【英文文摘】In this communication, we obtained for the first time the SERS spectra of gold at the laser excitation of 514.5 nm by fabricating the ordered array of gold nanoparticles with special shapes, e.g., sphere, tadpole, and pearlchain. Selfassemble method of these gold nanoparticles is briefly introduced. In general, the enhancement of gold by the laser excitation of short wavelength (600 nm) becuase that the coupling between conduction electrons and interband electronic transitions by using 514. 5 nm depresses the quality of the surface plasmon resonance of gold metals considerably. Our observation reveals that the SERS of self2assembly gold nanoparticles with special shape can be rather high even using the green light excitation , which is mainly due to the lightning2rod effect.国家自然科学基金创新群体研究资助项目(20021002);; 国家自然科学基金九五重点项目(29833060

粗糙铂电极上甲酸吸附氧化的电化学原位表面增强拉曼光谱研究

采用循环伏安法和电化学原位表面增强拉曼光谱 (SERS)技术研究甲酸的解离吸附与氧化行为 .首次报道了甲酸吸附、解离和氧化的电化学原位SERS谱 ,发现甲酸在粗糙铂电极上能自发解离吸附 ;首次成功地获得了粗糙铂电极上甲酸吸附解离的强吸附中间体CO和活性中间体COOH的表面增强拉曼光谱 ,同时首次检测到甲酸氧化最终产物CO2 的拉曼光谱信号 ,从分子水平证实甲酸解离吸附反应的双途径机理

Size Distribution of Particle and Polycyclic Aromatic Hydrocarbons in Particle Emissions from Simulated Emission Sources

采用再悬浮箱模拟得到不同粒径的烹调油烟、生物质和塑料燃烧烟尘、汽车尾气和发电机烟气等颗粒,并用gC/MS对不同粒径颗粒中18种多环芳烃(PAHS)进行了分析.结果表明,烹调油烟中颗粒物存在0.44~1.0μM和2.5~10μM两个峰值,稻草和木材燃烧排放烟尘只有0.44~1.0μM一个峰值,塑料燃烧排放烟尘的峰值不明显,汽车尾气尘因含有大量的水汽导致其粒径峰值出现在2.5~10μM,而发电机排放的烟尘约93%集中在≤2.5μM的粒径范围.烹调油烟和汽车尾气尘中低环数PAHS在2.5~10μM范围内的峰值明显;随环数增加,0.44~1.0μM范围内的峰值变得明显;不同排放源亚微米颗粒中单一PAH占全部颗粒态中该PAH的比例都呈现随分子量的增大而增大的趋势.烹调油烟和燃烧排放颗粒中PAHS的组成以菲占主导,但汽车尾气和发电机烟尘中含量最高的PAHS分别是萘和苯并[g,H,I]苝.来源特征比值的比较显示,烹调油烟与生物质燃烧颗粒中PAHS的源特征较为接近,但两者都不同于汽车尾气和发电机烟尘.Particles from cooking lampblack,biomass and plastics burning smoke,gasoline vehicular exhausts and gasoline generator exhausts were prepared in a resuspension test chamber and collected using a cascade MOUDI impactor.A total of 18 polycyclic aromatic hydrocarbons( PAHs) associated with particles were analyzed by GC-MS.The results showed that there were two peaks in the range of 0.44-1.0 μm and 2.5-10 μm for cooking lampblack,and only one peak in the range of 0.44-1.0 μm for straw and wood burning smoke.But there were no clear peak for plastics burning smoke.The peak for gasoline vehicular exhausts was found in the range of 2.5-10 μm due to the influence of water vapor associated with particles,while the particles from gasoline generator exhausts were mainly in the range of ≤2.5 μm( accounting for 93% of the total mass).The peak in 2.5-10 μm was clear for cooking lampblack and gasoline vehicular exhausts.The peak in the range of 0.44-1.0 μm became more and more apparent with the increase of PAHs molecular weight.The fraction of PAH on particles less than 1.0 μm to that on the total particles increased along with PAH's molecular weight.Phenanthrene was the dominant compound for cooking lampblack and combustion smoke,while gasoline vehicular exhausts and generator exhausts were characterized with significantly high levels of naphthalene and benzo[g,h,i]perylene, respectively.The distribution of source characteristic ratios indicated that PAHs from cooking lampblack and biomass burning were close and they were different from those of vehicular exhausts and generator exhausts.国家自然科学基金项目(40971257;41171365); 环境保护公益性行业科研专项(201009004

一种检测蓝藻质粒的定量PCR试剂盒及应用

本发明公开了一种检测蓝藻质粒的定量PCR试剂盒及应用，普通PCR反应液I包含针对聚球藻anl50基因设计的一对特异性引物，普通PCR反应液II包含针对聚球藻CDS：ABB57481.1基因设计的一对特异性引物，定量PCR反应液I包含针对聚球藻anl50基因设计的一对特异性引物，定量PCR反应液II中包含针对聚球藻CDS：ABB57481.1基因设计的一对特异性引物，其中扩增anl50基因的普通PCR上游引物与该基因的结合位置在其定量PCR上游引物与该基因结合位置的上游，anl50基因的普通PCR下游引物与该基因的结合位置在其定量PCR下游引物与该基因结合位置的下游。该试剂盒快速检测蓝藻质粒的拷贝数，具有高灵敏度、特异性、稳定性和重现性。适用于蓝藻质粒DNA的定量检测，对于检测蓝藻细胞的DNA损伤具有实际的应用价值。</p

In-situ SERS study on the electro-oxidation with HCOOH on a roughened platinum electrode

The dissociative adsorption and oxidation behavior of HCOOH on Pt was investigated by cyclic voltammogram (CV) and in-situ surface enhanced Raman spectroscopy (SERS) techniques. The in-stiu SERS of HCOOH adsorption, dissociation and oxidation on rough Pt is reported. It is found that HCOOH can spontaneously dissociate. The surface Raman spectra of CO, the strongly adsorbed intermediate and COOH, the weakly adsorbed intermediate of the dissociative adsorption of HCOOH were successfully obtained for the first time. At the same time, the Raman spectra of the finally oxidized product CO2 of HCOOH was also firstly detected. The dual path reaction mechanism for the oxidation of HCOOH was confirmed at molecular level