Research on control system of meibomian gland massage instrument based on C8051F960 MCU

睑板腺功能障碍是引起蒸发过强型干眼症的主要原因。在我国，睑板腺功能障碍的发病率较高。本文针对睑板腺功能障碍治疗中最重要的步骤——热敷和睑板腺按摩，进行机电一体化研究，在已初步完成睑板腺按摩仪机械结构的基础上，进行睑板腺按摩仪控制系统研究，完成了相应控制电路板以及软件方案的设计，并进行了睑板腺按摩仪控制手柄外形设计。 该睑板腺按摩仪，通过按键进行控制，通过显示屏进行显示，同时具有非常人性的按键功能播报以及按摩环境参数播报功能，方便用户能够快速掌握按摩仪的使用。 睑板腺按摩仪控制系统包含以下模块：按键模块、主控MCU、电源模块、电机驱动模块、温度控制模块、压力控制模块、显示屏驱动模块、语音控制...Meibomian gland dysfunction is the main reason that cause evaporative dry eye,it has a higher incidence in China.Meibomian gland fomentation and massage is the most important steps for Meibomian gland dysfunction treatment. For this point, we conducted a electromechanical integration research. On the base of the completion of the massage structure, we study the control system of meibomian gland ma...学位：工程硕士院系专业：航空航天学院_工程硕士(机械工程)学号：1992013115293

Synonymous Codon Usage of Both Alternativelyand Commonly Spliced Genes in Human Chromosome 1 I: Synonymous Codon Usage Bias Analysis

人类1号染色体可变剪接(选择性剪接)基因344非冗余蛋白质编码序列(188183密码子)和普通剪接(非可变剪接)基因的386蛋白质编码序列(223116密码子)被用于研究人类密码子使用偏爱模式。全部密码子使用数据分析表明,人类可变剪接基因密码子的偏爱水平显著高于普通剪接基因。在人类1号染色体基因中,密码子第三位置的G+C含量有很大的异质性(0 24～0 95),并且可变剪接基因密码子第三位置平均G+C含量(64 66%)大于普通剪接基因(59 97%)。Nc值对GC3s图显示密码子偏爱使用除了受核苷酸组成制约外,其它的因子可能也影响密码子的使用变化。此外,可变剪接基因中以G或C结尾的密码子比普通剪接基因出现的频率高。密码子使用的差异可能是由可变剪接基因pre mRNA特有的结构特征和多种剪接模式决定的。It is already clear that alternative splicing has an extremely important role in expanding the protein diversity. Comparative study of the codon usage patterns of alternatively and commonly spliced genes may thereby be necessary. In this paper, the patterns of codon usage bias of two kinds of human genes, alternatively spliced genes and commonly spliced genes, were formulated through analyzing 344 non-redundant protein coding sequences from alternatively spliced genes (188183 codons) and 386 from commonly spliced genes (223116 codons) in human chromosome (1. Overall) codon usage data analysis indicated that the alternatively spliced genes showed a stronger codon usage bias than commonly spliced genes. Very extensive heterogeneity of G+C content in silent third codon position (GC3s) was evident among these genes, and GC3s content of alternatively spliced genes was higher than that of commonly spliced genes. G- or C-ending codons were more abundant in alternatively spliced genes than commonly spliced genes in human chromosome 1. The causation of differences created could be explained by pre-mRNA structural characteristics of alternatively spliced genes influencing their codon usage bias.Chinapostdoctoralprogramsfoundation( 2 0 0 2 11

Synonymous Codon Usage of Both Alternativelyand Commonly Spliced Genes in Human Chromosome 1 II: Expression Pattern and Gene Length Shape Codon Usage

进一步研究基因表达水平和基因长度与密码子使用偏爱之间的关系。多变量统计分析发现,人类1号染色体选择性剪接基因和普通剪接基因密码子使用变化都呈现单一趋势,且它们之间的密码子使用模式也非常相似,推测的高表达基因确实偏爱以C或G结尾的密码子,基因表达水平与密码子使用偏爱之间的关联也达到显著水平。因此,人类1号染色体高表达基因密码子的使用偏爱可能主要被翻译选择所决定。此外,基因长度与密码子偏爱水平之间也存在高度相关,说明相对较短的基因具有较高的密码子使用偏爱,翻译选择可能缩短了高表达基因的长度从而提高翻译效率。We further explored the correlation between synonymous codon bias and gene expression level and gene length. Correspondence analysis showed that there was only a single trend in codon usage variation among alternatively and commonly spliced genes, and the pattern of codons usage was very similar in them. The putatively highly expressed genes preferred C- or G-ending codons, and the correlations between synonymous codon bias and gene expression levels were also very significant, which implied that translation selection might play an important role in dominating codon usage bias in highly expressed genes in humans. Surprisingly, a strong correlation between codon-usage bias and gene length was also observed, e.g. the shorter a gene′s length, the higher its expression capacity. It suggested that the selection of codons might result in the reduction of the length of highly expressed genes and enhancement of translational efficiency.Chinapostdoctoralprogramsfoundation( 2 0 0 2 11

Testing AnnAGNPS for water quality modelling in the typical sub-watersheds in Jiulong River watershed

在中国东南丘陵山区一中尺度流域———九龙江流域选取4个土地利用方式不一的典型小流域,借助GIS和相关资料率定模型参数,并通过对2003年4月至9月各小流域出口降雨水质水量的同步监测,检验了连续分布式参数模型(AnnualizedAGriculturalNonPointSourceModel,AnnAGNPS)的适用性.研究结果表明,模型对于地表径流量的模拟能力好于对泥沙和营养盐的模拟,对总磷和泥沙输出的模拟结果表现出了较大的不确定性. 【英文摘要】 An interface, in which ArcView Geographic Information System(GIS) was integrated with Annualized Agricultural Non-Point Source Model (AnnAGNPS), was used as a procedure to input, analyze, and visualize spatial data that agricultural Non-point source pollution concerned. Four typical sub-watersheds in southeast of China were chosen to test AnnAGNPS model from storm evens during the period of April to September, 2003. Then, the model was run to simulate the annual runoff, sediment and nutrient yield. The simu...福建省科技重大攻关项目(No.2002H009)~

Capturing the labile fullerene[50] as C50Cl10

地址: 1. Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Xiamen 361005, Peoples R China 3. Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 4. Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Mol Phys, Wuhan 430071, Peoples R China 电子邮件地址: [email protected]

Screening of adjuvant enhancing cellular immune response induced by ESAT6-CFP10 fusion protein in mice

通讯作者：叶祥忠，E-mail：[email protected][中文文摘] 目的 筛选能增强特异性抗原早期分泌抗原靶6蛋白(Early secretory antigenic target6,ESAT6)-培养滤出液蛋白-10(Culture filtrate protein10,CFP-10)融合蛋白(E1C0)诱导小鼠细胞免疫应答的佐剂,建立基于细胞免疫应答的小鼠模型,以评价基于体外干扰素γ释放分析(IFNγrelease assay,IGRA)结核诊断方法中特异性刺激抗原E1C0的活性。方法 建立小鼠IFNγ双抗体夹心SABC-ELISA检测系统,并验证系统的线性、灵敏度、重复性和特异性。将BALB/c小鼠随机分为7组:E1C0+单磷酸类脂A(Monophosphoryl lipid A,MPL)+双十八烷基二甲基溴化铵(Dimethyl dioctadecylammonium bromide,DDA)组、E1C0+DDA组、E1C0+MPL组、E1C0+弗氏不完全佐剂(IFA)组、E1C0组、生理盐水组和MPL+DDA联合组,每组6只,经小鼠后肢内侧皮下免疫3次,间隔2周,免疫剂量为:E1C0100滋g/只,MPL25μg/只,DDA250μg/只,IFA100滋l/只。末次免疫4周后处死小鼠,无菌取脾,分离脾淋巴细胞,加入E1C0进行培养,MTT法检测特异性淋巴细胞增殖反应,ELISA法检测培养上清中IFNγ水平。采用筛选出的最佳佐剂与抗原组合免疫3批BALB/c小鼠,进行IFNγ诱生测定。结果 检测系统的线性范围为:40～2560pg/ml(R>0.98);灵敏度为40pg/ml;变异系数(CV)0.05)。结论 E1C0与MPL和DDA联合免疫所诱导的小鼠Th1型细胞免疫应答最强,成功建立了用于评价刺激抗原E1C0活性的小鼠模型。[英文文摘]Objective To screen the adjuvant enhancing the cellular immune response induced by early secretory antigenic target 6（ESAT6）-culture filtrate protein-10（CFP10）in mice, and establish an animal model based on cellular immunγe response for evaluation of activity of specific stimulating antigen E1C0 in IFNγ release assay（IGRA）for diagnosis of tuberculosis（TB）. Methods mDouble antibody sandwich SABC-ELISA system for mouse IFNγ was developed and verified for linearity, sensitivity, reproducibility and specificity. BALB/c mice were randomly divided into seven groups, 6 for each, and immunized s.c. with E1C0 + monophosphoryl lipid A（MPL）+ dimethyl dioctadecylammonium bromide（DDA）, E1C0 + DDA, E1C0 + MPL, E1C0 + IFA, E1C0, physiological saline and MPL + DDA for 3 times, respectively, each at an interval of 2 weeks. The dosages of E1C0, MPL, DDA and IFA for immunization were 100 μg, 25μg, 250μg and 100 μl, respectively. The mice were killed 4 weeks after the last immunization, and their spleens were collected aseptically, from which splenic lymphocytes were isolated, cultured with E1C0, then determined for proliferation level by MTT method, and for IFNlevel in culture supernatant by ELISA. Three batches of BALB/c mice were immunized with the screened adjuvant combined with antigen, and determined for IFNγ induced. Results The linear range, sensitivity and CV value of developed SABC-ELISA system were 40 ~ 2 560 pg / ml（R > 0. 98）, 40 μg/ml and less than 15%respectively, by which all the detection results of IFN酌in rat, guinea pig and rabbit sera were negative. The stimulating indexox（SIs） of specific lymphocyte proliferation in E1C0 + MPL + DDA, E1C0 + IFA and E1C0 + DDA groups were significantly higher than those in physiological saline group （P < 0. 01）. The IFN酌level secreted by lymphocytes in E1C0 + MPL + DDA group after stimulation with E1C0 in vitro was significantly higher than those in other groups （P < 0. 001）. No significant differences were observed in IFNγ levels induced in 3 batches of mice in E1C0 + MPL + DDA group（P > 0. 05）. Conclusion The immunization with E1C0 in a combination with MPL and DDA elicited a strong Th1 cellular immune response in mice. Mouse model for evaluation of activity of E1C0 antigen was successfully established

中国物理海洋学研究70年：发展历程、学术成就概览

本文概略评述新中国成立70年来物理海洋学各分支研究领域的发展历程和若干学术成就。中国物理海洋学研究起步于海浪、潮汐、近海环流与水团,以及以风暴潮为主的海洋气象灾害的研究。随着国力的增强,研究领域不断拓展,涌现了大量具有广泛影响力的研究成果,其中包括:提出了被国际广泛采用的"普遍风浪谱"和"涌浪谱",发展了第三代海浪数值模式;提出了"准调和分析方法"和"潮汐潮流永久预报"等潮汐潮流的分析和预报方法;发现并命名了"棉兰老潜流",揭示了东海黑潮的多核结构及其多尺度变异机理等,系统描述了太平洋西边界流系;提出了印度尼西亚贯穿流的南海分支(或称南海贯穿流);不断完善了中国近海陆架环流系统,在南海环流、黑潮及其分支、台湾暖流、闽浙沿岸流、黄海冷水团环流、黄海暖流、渤海环流,以及陆架波方面均取得了深刻的认识;从大气桥和海洋桥两个方面对太平洋–印度洋–大西洋洋际相互作用进行了系统的总结;发展了浅海水团的研究方法,基本摸清了中国近海水团的分布和消长特征与机制,在大洋和极地水团分布及运动研究方面也做出了重要贡献;阐明了南海中尺度涡的宏观特征和生成机制,揭示了中尺度涡的三维结构,定量评估了其全球物质与能量输运能力;基本摸清了中国近海海洋锋的空间分布和季节变化特征,提出了地形、正压不稳定和斜压不稳定等锋面动力学机制;构建了"南海内波潜标观测网",实现了对内波生成–演变–消亡全过程机理的系统认识;发展了湍流的剪切不稳定理论,提出了海流"边缘不稳定"的概念,开发了海洋湍流模式,提出了湍流混合参数化的新方法等;在海洋内部混合机制和能量来源方面取得了新的认识,并阐述了混合对海洋深层环流、营养物质输运等过程的影响;研发了全球浪–潮–流耦合模式,推出一系列海洋与气候模式;发展了可同化主要海洋观测数据的海洋数据同化系统和用于ENSO预报的耦合同化系统;建立了达到国际水准的非地转(水槽/水池)和地转(旋转平台)物理模型实验平台;发展了ENSO预报的误差分析方法,建立了海洋和气候系统年代际变化的理论体系,揭示了中深层海洋对全球气候变化的响应;初步建成了中国近海海洋观测网;持续开展南北极调查研究;建立了台风、风暴潮、巨浪和海啸的业务化预报系统,为中国气象减灾提供保障;突破了国外的海洋技术封锁,研发了万米水深的深水水听器和海洋光学特性系列测量仪器;建立了溢油、危险化学品漂移扩散等预测模型,为伴随海洋资源开发所带来的风险事故的应急处理和预警预报提供科学支撑。文中引用的大量学术成果文献(每位第一作者优选不超过3篇)显示,经过70年的发展,中国物理海洋学研究培养了一支实力雄厚的科研队伍,这是最宝贵的成果。这支队伍必将成为中国物理海洋学研究攀登新高峰的主力军

一种接触区可调的曲线圆柱齿轮成形方法与理论验证

为了提升曲线圆柱齿轮副的啮合性能，提出了一种可调整齿面接触区的成形方法。首先，制定了调整齿面接触区的基本方案，即，大齿轮采用双刃面铣刀加工，小齿轮凹齿面和凸齿面分别采用半径可调的外刃和内刃面铣刀加工；其次，基于啮合理论，推导了齿轮副的齿面数学模型；然后，基于轮齿接触分析（Tooth contact analysis，TCA），建立了装配误差下齿面接触和传动误差的数学模型；最后，通过8组算例从理论上验证了所提出方案的可行性。研究结果表明,所提出的成形方法是可行的，调整刀盘曲率半径可形成任意长度接触区的齿轮副，较大的齿面接触区可以提升齿轮的力学性能，但是齿面接触区过大会增加传动误差的敏感性

装配误差和齿面修形对曲线圆柱齿轮传动误差的影响

基于齿轮啮合理论，推导了带抛物线修形的曲线圆柱齿轮齿面方程，研究了齿轮副有限元模型初始接触位置的求解方法；利用齿轮参数化有限元技术，建立了齿轮副的有限元模型，从有限元分析结果中提取了齿轮载荷传动误差；通过20余组算例分别研究了齿轮副的装配误差、修形量、刀盘曲率半径和修形曲线偏置量对载荷传动误差的影响。结果表明，装配误差可以降低载荷传动误差的波动幅度；较大的齿面修形量可减小载荷传动误差波动幅度；较大的刀盘曲率半径可减小载荷传动误差值和误差波动幅度；齿面修形曲线的偏置对载荷传动误差的影响较小。总的来说，采用较大曲率半径的修形刀盘可加工出传动性能更好的曲线圆柱齿轮

Dynamics of soil organic carbon in Caragana microphylla forest and its relationship with environment factors in loess hilly region

以黄土丘陵区柠条人工林为研究对象,采用野外调查与室内分析相结合的方法,探讨柠条生长过程中土壤有机碳储量的变化规律。结果表明:1)土壤有机碳主要分布在0～20 cm土层,占0～50 cm土层总储量的49%～63%;2)相对于对照地,柠条林地土壤有机碳储量随柠条生长年限的增加先减小再升高最后趋于稳定,10、26、40、50 a柠条林地土壤总有机碳储量分别为1.555、3.236、2.775、2.444 kg/m2,26 a林地土壤有机碳储量最高,随林龄增大其变化趋于稳定;3)相关性分析结果表明,土壤有机碳质量分数与土壤密度之间呈显著负相关关系,各林地土壤密度随柠条生长年限的增加而减小,说明柠条可以通过改变土壤性质间接增加土壤总有机碳储量,土壤有机碳质量分数与根系生物量、土壤全氮质量分数之间呈极显著正相关关系,说明柠条的根系生长和固氮特性有助于有机碳的积累