“象”“症”结合论治肿瘤探析

王彦晖教授经过长期临床实践与观察提出了癌症的\"种子土壤说\"新论。强调舌象和脉象是判断肿瘤病机的重要指标,\"象\"与\"症\"的结合更是临床上辨证论治的重要手段。理气疏肝、祛痰化湿、活血化瘀是治疗肿瘤的核心。通过剖析临证治疗肿瘤的实例经验,以期对临床治疗和预防肿瘤具有参考意义。厦门市重大科技计划项目(No.3502Z20100006);;厦门市科技计划高校创新项目(No.3502Z20153027)~

中医现代化新论

中医学的传承和发展,核心是要实现中医现代化,关键是找到真正适合中医现代化的切入点。根源在于如何认识中医现代化的本质、中医现代化的必要性、实现中医现代化的方法以及中医现代化的意义。文章通过对以上4个层次的阐述,以及对其间沟通联系形成认识体系的解读,以期对中医学的发展有一定的启发意义。厦门市重大科技计划项目(No.3502Z20100006);;厦门市科技计划高校创新项目(No.3502Z20153027)~

王彦晖运用健脾补气法治疗肥胖病经验解析

肥胖对人体健康的影响已经成为全球性医学社会问题,是现代人类多种慢性疾病的危险因素,对肥胖的治疗非常必要。王彦晖教授认为,脾失健运是肥胖病的关键病因,中医病机以虚为主,治以健脾补气;运用"健脾补气法",始终扣住恢复脾的功能这一中心环节,辅以饮食和运动指导,医患配合,疗效满意。厦门市重大科技计划项目(No.3502Z20100006);;厦门市科技计划高校创新项目(No.3502Z20153027)~

Screening of adjuvant enhancing cellular immune response induced by ESAT6-CFP10 fusion protein in mice

通讯作者：叶祥忠，E-mail：[email protected][中文文摘] 目的 筛选能增强特异性抗原早期分泌抗原靶6蛋白(Early secretory antigenic target6,ESAT6)-培养滤出液蛋白-10(Culture filtrate protein10,CFP-10)融合蛋白(E1C0)诱导小鼠细胞免疫应答的佐剂,建立基于细胞免疫应答的小鼠模型,以评价基于体外干扰素γ释放分析(IFNγrelease assay,IGRA)结核诊断方法中特异性刺激抗原E1C0的活性。方法 建立小鼠IFNγ双抗体夹心SABC-ELISA检测系统,并验证系统的线性、灵敏度、重复性和特异性。将BALB/c小鼠随机分为7组:E1C0+单磷酸类脂A(Monophosphoryl lipid A,MPL)+双十八烷基二甲基溴化铵(Dimethyl dioctadecylammonium bromide,DDA)组、E1C0+DDA组、E1C0+MPL组、E1C0+弗氏不完全佐剂(IFA)组、E1C0组、生理盐水组和MPL+DDA联合组,每组6只,经小鼠后肢内侧皮下免疫3次,间隔2周,免疫剂量为:E1C0100滋g/只,MPL25μg/只,DDA250μg/只,IFA100滋l/只。末次免疫4周后处死小鼠,无菌取脾,分离脾淋巴细胞,加入E1C0进行培养,MTT法检测特异性淋巴细胞增殖反应,ELISA法检测培养上清中IFNγ水平。采用筛选出的最佳佐剂与抗原组合免疫3批BALB/c小鼠,进行IFNγ诱生测定。结果 检测系统的线性范围为:40～2560pg/ml(R>0.98);灵敏度为40pg/ml;变异系数(CV)0.05)。结论 E1C0与MPL和DDA联合免疫所诱导的小鼠Th1型细胞免疫应答最强,成功建立了用于评价刺激抗原E1C0活性的小鼠模型。[英文文摘]Objective To screen the adjuvant enhancing the cellular immune response induced by early secretory antigenic target 6（ESAT6）-culture filtrate protein-10（CFP10）in mice, and establish an animal model based on cellular immunγe response for evaluation of activity of specific stimulating antigen E1C0 in IFNγ release assay（IGRA）for diagnosis of tuberculosis（TB）. Methods mDouble antibody sandwich SABC-ELISA system for mouse IFNγ was developed and verified for linearity, sensitivity, reproducibility and specificity. BALB/c mice were randomly divided into seven groups, 6 for each, and immunized s.c. with E1C0 + monophosphoryl lipid A（MPL）+ dimethyl dioctadecylammonium bromide（DDA）, E1C0 + DDA, E1C0 + MPL, E1C0 + IFA, E1C0, physiological saline and MPL + DDA for 3 times, respectively, each at an interval of 2 weeks. The dosages of E1C0, MPL, DDA and IFA for immunization were 100 μg, 25μg, 250μg and 100 μl, respectively. The mice were killed 4 weeks after the last immunization, and their spleens were collected aseptically, from which splenic lymphocytes were isolated, cultured with E1C0, then determined for proliferation level by MTT method, and for IFNlevel in culture supernatant by ELISA. Three batches of BALB/c mice were immunized with the screened adjuvant combined with antigen, and determined for IFNγ induced. Results The linear range, sensitivity and CV value of developed SABC-ELISA system were 40 ~ 2 560 pg / ml（R > 0. 98）, 40 μg/ml and less than 15%respectively, by which all the detection results of IFN酌in rat, guinea pig and rabbit sera were negative. The stimulating indexox（SIs） of specific lymphocyte proliferation in E1C0 + MPL + DDA, E1C0 + IFA and E1C0 + DDA groups were significantly higher than those in physiological saline group （P < 0. 01）. The IFN酌level secreted by lymphocytes in E1C0 + MPL + DDA group after stimulation with E1C0 in vitro was significantly higher than those in other groups （P < 0. 001）. No significant differences were observed in IFNγ levels induced in 3 batches of mice in E1C0 + MPL + DDA group（P > 0. 05）. Conclusion The immunization with E1C0 in a combination with MPL and DDA elicited a strong Th1 cellular immune response in mice. Mouse model for evaluation of activity of E1C0 antigen was successfully established

Capturing the labile fullerene[50] as C50Cl10

地址: 1. Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Xiamen 361005, Peoples R China 3. Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 4. Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Mol Phys, Wuhan 430071, Peoples R China 电子邮件地址: [email protected]

常压下高浓度NaOH浸取铝土矿预脱硅

探讨了常压下高浓度NaOH浸取铝土矿的预脱硅过程中初始NaOH浓度、反应温度、浸出时间和碱矿比等因素对氧化铝、氧化硅浸出率及剩余固相中铝硅比的影响,并得出动力学方程.结果表明,在50%NaOH溶液、碱矿比2.5及135℃浸出时,反应时间5～20min内,可使铝土矿铝硅比由7.6提高到12以上,从而满足拜尔法生产氧化铝对铝土矿的品位要求.用此方法处理铝土矿预脱硅,可以免去物理选矿环节,与其他化学选矿方法相比,具有节能降耗的优点,同时提高铝土矿品位,为中低品位铝土矿的开发利用开辟了一条新途径

常压下高浓度NaOH浸取铝土矿预脱硅

探讨了常压下高浓度NaOH浸取铝土矿的预脱硅过程中初始NaOH浓度、反应温度、浸出时间和碱矿比等因素对氧化铝、氧化硅浸出率及剩余固相中铝硅比的影响，并得出动力学方程．结果表明，在50％NaOH溶液、碱矿比2．5及135℃浸出时，反应时间5～20min内，可使铝土矿铝硅比由7．6提高到12以上，从而满足拜尔法生产氧化铝对铝土矿的品位要求．用此方法处理铝土矿预脱硅，可以免去物理选矿环节，与其他化学选矿方法相比，具有节能降耗的优点，同时提高铝土矿品位，为中低品位铝土矿的开发利用开辟了一条新途径