Influence of Helicobacter pylori infection on autologous DC-CIK maintenance therapy in the advanced gastric cancer patients

目的:对比幽门螺杆菌(HElICObACTEr PylOrI,HP)感染阴性和阳性的晚期胃癌患者接受自体树突状细胞联合细胞因子诱导的杀伤(dEndrITIC CEllS-CyTOkInE InduCEd kIllEr,dC-CIk)细胞维持治疗的疗效差异。方法:收集2010年6月至2012年6月中国人民解放军第174医院肿瘤中心收治的72例晚期胃癌患者,年龄29~90岁,中位年龄56岁,通过胃镜检查进行胃癌确诊并检测HP,分为HP阳性组(45例)及HP阴性组(27例),在接受手术或/和放化疗后,接受2疗程自体dC-CIk维持治疗,比较两组外周血培养的dC分化成熟情况及临床疗效差异。结果:两组dC成熟过程形态变化无差异;HP阳性组dC表面分子Cd83、Cd86表达显著高于HP阴性组(P0.05)。HP阳性组治疗后生活质量评分(kPS)、外周血T细胞亚群(Cd3+、Cd4+、Cd8+)比例较治疗前显著提高(P0.05)。HP阳性组治疗后kPS评分、CEA和CA199表达以及Cd3+和Cd4+T细胞数量等的改善均优于HP阴性组(P0.05)。共随访2年,阳性组患者中位生存期为12.64个月,长于阴性组的11.42个月(P 0.05).Levels of CD83 and CD86 on the surface of DCs were significantly higher in the Hp-positive group than those in the Hp-negative group( P 0.05).In the Hp-positive group,the quality of life KPS score and levels of T lymphocyte markers( CD3+,CD4+and CD8+) were significantly increased( P 0.05).Hp-positive patients had better KPS score,higher levels of CEA and CA199,and more abundant CD3+CD4+T cells than Hp-negative patients( P 0.05).At the end of 2-years follow-up,the median survival time was 12.64 months in the HP-positive group and 11.42 months in the HP-negative group( P < 0.05).Conclusion: Hp infection may help stabilize the tumor size,improve the quality of life,and prolong the survival time in patients with advanced gastric cancer undergoing maintenance therapy with DC-CIK cells.南京军区医学科技创新课题资助(No.10MA068); 厦门市科技计划创新项目资助(No.3502z20134026;No.3502z20144034)~

pJAK2 polypeptide,an antagonist of suppressors of cytokine signaling-1,can enhance the antitumor effect of dendritic cells

目的:探讨细胞因子信号转导抑制因子-1(SuPPrESSOrS Of CyTOkInE SIgnAlIng1,SOCS1)拮抗物PJAk2多肽(氨基酸序列号为1001-1013)参与树突状细胞(dEndrITIC CEllS,dCS)的体外诱导培养后对dCS抗肿瘤作用的影响。方法:采集健康人外周血,离心获得单个核细胞,用重组人粒细胞巨噬细胞集落刺激因子(rECOMbInAnT HuMAn grAnulOCyTE-MACrOPHAgE COlOny STIMulATIng fACTOr,rHgM-CSf)及重组人白细胞介素-4(rECOMbInAnT HuMAn InTErlEukIn-4,rHIl-4)诱导dCS,第5天分为4组:单纯dCS培养(对照)组、抗原负载(lySATE-dCS)组、多肽修饰(PJAk2-dCS)组和抗原与多肽共培养(lySATE+PJAk2-dCS)组,第6天各组加入肿瘤坏死因子-α(TuMOr nECrOSIS fACTOr-AlPHA,Tnf-α)促成熟。倒置显微镜下观察dCS形态;fCM法检测dCS表型;乳酸脱氢酶(lACTATE dEHydrOgEnASE,ldH)细胞毒实验检测各组细胞毒性T淋巴细胞(CyTOTOXIC T lyMPHOCyTE,CTl)对胃癌细胞bgC-823的靶向杀伤作用;ElISA法检测白细胞介素-12(InTErlEukIn-12,Il-12)和γ干扰素(InTErfErOn-γ,Ifn-γ)的水平。结果:与未加入诱导剂组相比,各组均成功诱导出成熟dCS,均高表达Cd80、Cd83、Cd86和人类白细胞dr抗原(HuMAn lEukOCyTE AnTIgEn dr,HlA-dr),但以lySATE+PJAk2-dCS组的表达水平最高。在10:1~30:1的效靶比范围内,CTl杀伤作用与效靶比呈正相关。当效靶比为30:1时,对照组的CTl杀伤率达(19.77±2.34)%,低于其他3组(P<0.01),而lySATE+PJAk2-dCS组较lySATE-dCS组及PJAk2-dCS组都高(P<0.05)。lySATE+PJAk2-dCS组培养上清液中Il-12及Ifn-γ的分泌水平明显高于对照组(P<0.01)。结论:SOCS1拮抗物PJAk2多肽(1001-1013)可增强dCS对胃癌细胞的抗原递呈及特异性抗肿瘤作用。Objective:To investigate the effect of pJAK2 polypeptide,an antagonist of SOCS1(suppressors of cytokine signaling 1),on antitumor effect of in vitro cultivation-induced DCs(dendritic cells).Methods:Peripheral blood was collected from the healthy volunteers,and the PBMCs(peripheral blood mononuclear cells)were isolated.DCs were induced by rhGM-CSF(recombinant human granulocyte-macrophage colony-stimulating factor)and rhIL-4(recombinant human interleukin-4).On the fifth day,DCs were divided into four groups:control group,Lysate-DCs group,pJAK2-DCs group,and Lysate + pJAK2 DCs group.On the sixth day,TNF-α(tumor necrosis factor-alpha)was added into each group.The morphological features of DCs were observed under an inverted microscope;the phenotypes were detected by FCM(flow cytometry);the killing effect of CTLs(cytotoxic T lymphocytes)on gastric cancer BGC-823 cells was evaluated by LDH(lactate dehydrogenase)cytotoxicity test;the concentrations of IL-12(interleukin-12)and IFN-γ(interferon-γ)were detected by ELISA(enzyme-linked immuno sorbent assay).Results:Mature DCs presented typically morphological and phenotypic features;the DCs in Lysate + pJAK2-DCs group had the highest expression levels of CD80,CD83,CD86 and HLA-DR(human leukocyte antigen DR).When the ratio of effectors to target cells ranged from 10:1 to 30:1,the killing activity of CTLs had a positive correlation with the ratio.When the ratio of effectors to target cells was 30:1,the killing activity of CTLs in the control group was(19.77±2.34)%,which was lowest as compared with the other groups(P < 0.01),meanwhile the killing activity of CTLs in Lysate + pJAK2-DCs group was higher than those in Lysate-DCs and pJAK2-DCs groups(P < 0.05).The levels of IL-12 and IFN-γ secretion in Lysate + pJAK2-DCs group were apparently higher than those in the control group(P < 0.01).Conclusion:An antagonist of SOCS1,pJAK2 polypeptide,can enhance the ability of antigen presentation and specific antitumor effect of DCs on gastric cancer cells.南京军区医学科技创新课题资助项目(编号:10MA068); 福建省自然科学基金面上项目(编号:2010D013); 厦门市科技计划创新项目(编号:3502z20104014

2019年秋季鲍鱼生产形势调研分析

福建、山东、辽宁、浙江、广东和海南是全国鲍主要养殖省。本文介绍了全国鲍主产区分布及总体情况,分析了秋季鲍生产形势及特点,指出了存在的问题,并给出了合理建议。为全面了解2019年全国鲍秋季养殖生产形势及发展趋势,9月下旬至10月中旬,鲍鱼调研组采取电话调查、问卷调查与现场调研的方式,对福建漳州、莆田、福州、宁德和平

三叶因子1表达与胃黏膜损伤及胃癌的关系

目的　测定三叶因子 1(TFF1)在正常及病理条件下胃黏膜中的表达情况 ,探讨TFF1在胃黏膜损伤修复及胃癌抑制中的作用及意义。方法　应用免疫组化方法测定正常及不同病理条件下胃黏膜中TFF1的表达情况 ,通过图像分析软件分析阳性信号平均吸光度值以了解其表达情况。结果　胃炎、胃溃疡及十二指肠球部溃疡患者TFF1表达明显高于正常胃黏膜 (0 .5 1± 0 .0 5 ,0 .5 1± 0 .0 6 ,0 .5 0± 0 .0 6比 0 .4 4± 0 .0 6 ;P值均 0 .0 5 )。结论　TFF1表达随黏膜损伤程度的加重而表达增强 ,提示其在胃黏膜保护及促进上皮重建机制中具有一定的作用。TFF1在癌旁组织中表达增强提示其可能与肿瘤抑制及分化机制有关 ,而在癌组织中表达减弱可能与其分泌减少有关

Effect of Helicobacter pylori on the function of peripheral blood monocyte-derived dendritic cells in gastric cancer patients

分析HP阳性和HP阴性的胃癌患者外周血单核细胞来源树突状细胞(MOdCS)功能的差异性及其临床意义。方法:用尿素14C呼气试验对解放军第一七四医院2011年1月至2012年10月收治的84例胃癌患者进行HP感染状态鉴定,分别采集HP阳性和阴性胃癌患者外周血,分离外周血单个核细胞,采用经典方法(rHgM-CSf、rHIl-4联合rHTnf-α)诱导产生dCS,采用流式细胞仪检测dCS表型,采用ldH释放法检测dCS致敏T细胞对胃癌细胞的毒性杀伤作用,采用ElISA方法检测细胞因子Il-12、Ifn-γ的分泌水平。结果:两组MOdCS成熟过程形态变化无差异,HP阳性组MOdCS表面标记分子Cd1A、Cd80、Cd83、Cd86和HlA-dr平均表达百分率均高于HP阴性组,其中Cd80、Cd83、Cd86的表达水平差异有统计学意义,Cd1A、HlA-dr差异无统计学意义。HP阳性组dCS致敏T淋巴细胞对胃癌细胞杀伤率和Il-12、Ifn-γ的分泌水平均高于HP阴性组(P<0.05)。结论:HP感染状态不影响胃癌患者外周血MOdCS成熟过程形态变化,HP感染的胃癌患者MOdCS成熟和活化水平更高。Objective:This study aimed to compare and analyze the functional differences between peripheral blood monocyte-derived dendritic cells(DCs) of Helicobacter pylori-positive and H.pylori-negative patients with gastric cancer.Methods:H.pylori infection was detected in 84 patients with gastric cancer in our hospital from January 2011 to October 2012 by the14C-urea breath test.DCs were generated from monocytes isolated by an adherent method from the two groups of patients and cultured in the presence of rhIL-4,rhGM-CSF,and rhTNF-α.Furthermore,the expression of surface marker molecules was determined by fluorescence-activated cell sorting analysis.The cytotoxicity of DCs pulsed T cells against gastric carcinoma cell was assessed by the lactate dehydrogenase-releasing assay.The secretion of IL-12 and IFN-γ in the supernatant was determined by enzyme-linked immunosorbent assay.Results:No difference was observed in the morphological change of the maturation process.The mean expression of CD1a,CD80,CD83,CD86,and HLA-DR molecules in DCs of H.pylori-infected patients was higher than that in DCs of H.pylori-negative group,and the differences were statistically significant except for CD1a and HLA-DR.The cytotoxicity activities,IL-12 release,and IFN-γ release in the H.pylori-positive group were significantly higher than those in the H.pylori-negative group(P<0.05).Conclusion:H.pylori infection has no effect on the morphological change of the maturation process of monocyte-derived DCs.These data clearly demonstrate that monocyte-derived DCs of H.pylori-infected patients with gastric cancer can induce stronger maturation and activation than those of H.pylori-negative patients.南京军区医学科技创新课题(编号:10MA068); 福建省自然科学基金资助项目(编号:2010D013); 厦门市科技计划创新项目(编号:3502z20104014)资助~

东湖主要湖区浮游植物群落结构特征及其与环境因子的关系

目的了解东湖浮游植物的分布特征,为今后东湖的水资源开发与水环境保护提供生物学依据。方法2010年对武汉东湖4个主要子湖的浮游植物和环境因子进行每月调查。结果共检出浮游植物7门123种（绿藻58种,硅藻26种,蓝藻16种,裸藻14种,其他9种）,4个子湖中蓝藻、绿藻和硅藻的数量之和占总数量的98%以上,为东湖的优势藻门;东湖不同子湖的Shannon-W iener多样性指数均在1～3,属中污水质,湖泊水质为牛巢湖〉汤菱湖〉郭郑湖〉后湖;CCA排序分析表明东湖水体中浮游植物的空间分布主要受硝态氮和氨氮的影响,说明东湖是一个氮营养盐限制型湖泊;对比郭郑湖2010年与20世纪80年代的生物和理化指标,发现水体中硝态氮、氨氮含量和蓝绿藻的现存量都有显著提高。结论近年来,东湖从磷限制转变为氮限制型湖泊,富营养化正在日益加剧

东湖主要湖区浮游植物群落结构特征及其与环境因子的关系

[目的]了解东湖浮游植物的分布特征,为今后东湖的水资源开发与水环境保护提供生物学依据。[方法]2010年对武汉东湖4个主要子湖的浮游植物和环境因子进行每月调查。[结果]共检出浮游植物7门123种(绿藻58种,硅藻26种,蓝藻16种,裸藻14种,其他9种),4个子湖中蓝藻、绿藻和硅藻的数量之和占总数量的98%以上,为东湖的优势藻门;东湖不同子湖的Shannon-W iener多样性指数均在1～3,属中污水质,湖泊水质为牛巢湖>汤菱湖>郭郑湖>后湖;CCA排序分析表明东湖水体中浮游植物的空间分布主要受硝态氮和氨氮的影响,说明东湖是一个氮营养盐限制型湖泊;对比郭郑湖2010年与20世纪80年代的生物和理化指标,发现水体中硝态氮、氨氮含量和蓝绿藻的现存量都有显著提高。[结论]近年来,东湖从磷限制转变为氮限制型湖泊,富营养化正在日益加剧

东湖主要湖区浮游植物群落结构特征及其与环境因子的关系

目的了解东湖浮游植物的分布特征,为今后东湖的水资源开发与水环境保护提供生物学依据。方法2010年对武汉东湖4个主要子湖的浮游植物和环境因子进行每月调查。结果共检出浮游植物7门123种（绿藻58种,硅藻26种,蓝藻16种,裸藻14种,其他9种）,4个子湖中蓝藻、绿藻和硅藻的数量之和占总数量的98%以上,为东湖的优势藻门;东湖不同子湖的Shannon-W iener多样性指数均在1～3,属中污水质,湖泊水质为牛巢湖〉汤菱湖〉郭郑湖〉后湖;CCA排序分析表明东湖水体中浮游植物的空间分布主要受硝态氮和氨氮的影响,说明东湖是一个氮营养盐限制型湖泊;对比郭郑湖2010年与20世纪80年代的生物和理化指标,发现水体中硝态氮、氨氮含量和蓝绿藻的现存量都有显著提高。结论近年来,东湖从磷限制转变为氮限制型湖泊,富营养化正在日益加剧

2006年我院药品不良反应分析

对2006年我院上报福建省ADR监测中心的135例ADR,从涉及ADR的药品种类、剂型、生产厂家以及报告人身份、不良反应类别等方面进行回顾性统计分析。报告涉及药物83种,抗菌药物和中药制剂占前两位;ADR类型主要表现皮肤及其附件损害

东湖主要湖区浮游植物群落结构特征及其与环境因子的关系

目的了解东湖浮游植物的分布特征,为今后东湖的水资源开发与水环境保护提供生物学依据。方法2010年对武汉东湖4个主要子湖的浮游植物和环境因子进行每月调查。结果共检出浮游植物7门123种（绿藻58种,硅藻26种,蓝藻16种,裸藻14种,其他9种）,4个子湖中蓝藻、绿藻和硅藻的数量之和占总数量的98%以上,为东湖的优势藻门;东湖不同子湖的Shannon-W iener多样性指数均在1～3,属中污水质,湖泊水质为牛巢湖〉汤菱湖〉郭郑湖〉后湖;CCA排序分析表明东湖水体中浮游植物的空间分布主要受硝态氮和氨氮的影响,说明东湖是一个氮营养盐限制型湖泊;对比郭郑湖2010年与20世纪80年代的生物和理化指标,发现水体中硝态氮、氨氮含量和蓝绿藻的现存量都有显著提高。结论近年来,东湖从磷限制转变为氮限制型湖泊,富营养化正在日益加剧