Identification of Vibrio tubiashii isolated from diseased pond-cultured sea cucumbers( Apostichopus japonicus)

2013年春季,福建漳州地区池塘养殖刺参(APOSTICHOPuS JAPOnICuS)感染不明病原而患病。病参表现出排脏、身体萎缩、体表溃烂等症状,俗称为“腐皮综合征“。为确定引起该病的病原菌,从患病刺参病灶部位分离得到1株优势菌fJy001,经回接感染试验,证实所分离的细菌为刺参的病原菌。经形态、生理生化、16S rrnA序列分析和bIOlOg微生物自动鉴定系统等多项指标鉴定,确定该病原菌为塔式弧菌(VIbrIO TubIASHII)。In the spring of 2013,the pond-cultured sea cucumbers( Apostichopus japonicus) were infected by an unrestrained pathogen in Zhangzhou City of Fujian Province.The sea cucumbers with the disease tentatively termed as"skin ulcerative syndrome"showed symptoms such as viscera ejection,general atrophy and skin ulceration.To determine the bacterial pathogen that caused the disease,one strain( FJY001) was isolated from the lesions of diseased sea cucumbers.Pathogenicity of the strain was confirmed by artificial infection and the strain was identified as Vibrio tubiashii by indicators of morphological,physiological and biochemical characteristics as well as sequencing of 16 S rRNA and identification of Biolog microbiol system.福建省科技厅公益类科研专项(2010R1002-3); 福建省水产研究所科研发展基金资助项目(2014fjscq04

一种薄片抛磨机

ZL201910450460.7一种薄片抛磨机-虞钢、董斌鑫、何秀

用SSR标记检测同源四倍体与二倍体水稻的遗传差异

随机选用分布于水稻(Oryza stativa L.)12条染色体上的15对SSR(simple sequence repeats)引物,对18种中科院成都生物所培育的四倍体水稻和9种大面积种植的二倍体水稻进行了SSR多态性分析.11对具多态性的引物共检测到33条多态性条带,平均每对引物检测到3个等位基因.研究结果发现,所用同源四倍体水稻的基因组与二倍体水稻基因组大部分相同,只是在某些位点上具有差异,并筛选出部分SSR标记来区分二倍体与其同源四倍体.本研究还对二倍体与同源四倍体之间遗传差异的原因进行了初步的探讨

Effect of oscillation parameters on weld surface morphology during laser beam oscillation welding of stainless steel

During laser beam oscillation welding (LBOW), the weld surface morphology is important because it influences the appearance of the workpiece and reflects the welding quality, and the oscillation parameters have an important effect on the weld surface morphology. Therefore, a combination of experimental research and numerical simulation is conducted to investigate the influence of oscillation parameters, including oscillation mode, oscillation frequency, and oscillation amplitude, on the weld surface morphology during LBOW. The calculation encompasses the oscillation trajectory, combined velocity, and energy distribution on the weld surface, varying with different oscillation parameters. A three-dimensional heat transfer and fluid flow model with the oscillation heat source is established to simulate the temperature and fluid flow behavior inside the weld pool. When the heat input is constant, an increase in oscillation frequency results in a decrease in weld width and a smoother surface. As the oscillation frequency increases, the weld width decreases, the surface becomes smooth, and the energy distribution on the weld surface becomes more uniform. The fact that the period of the surface pattern is almost equal to the oscillation period indicates a direct correlation between the formation of weld surface pattern and the oscillation trajectory in LBOW. Among all the oscillation modes, the minimum combined velocity which is equal to the welding velocity occurs at the inflection point in the linear oscillation mode, and the variation in combined velocity of the laser beam is the smallest for circular oscillation mode. The surface pattern is primarily influenced by the oscillation trajectory and fluid flow within the weld pool. Under conditions of lower oscillation frequencies or larger oscillation amplitudes, the fluid flow is weaker, and the surface pattern is primarily influenced by the oscillation trajectory. Conversely, when the fluid flow is stronger, the surface pattern is mainly determined by the fluid flow within the weld pool under conditions of higher oscillation frequencies or smaller oscillation amplitudes. This study explains the formation mechanisms of weld surface patterns and provides insights and guidance for selecting optimal oscillation parameters in LBOW

基于Udwadia-Kalaba理论的四足机器人单腿动力学分析

处理多体系统动力学问题的传统方法是拉格朗日方法，但是在求解过程中会引入拉格朗日乘子，增加了求解显式动力学方程的难度。针对四足机器人动力学建模复杂的问题，基于Udwadia-Kalaba（UK）理论研究了四足机器人的单腿模型，建立了一种求解其显式动力学方程的新方法。为了简化获得方程的过程，使用UK方程来处理运动约束。考虑到真实环境的不确定性，利用滑模控制原理设计了一个非线性控制器来跟踪运动轨迹。数值模拟结果证明了动力学方程与控制器的正确性和有效性

Analysis and identification of causative pathogen for skin ulcerative syndrome in Fujian sea cage cultured Apostichopus japonicus

2011年冬季,福建省宁德市霞浦县海区网箱养殖刺参发生“腐皮综合症“,并伴有死亡现象出现。刺参的发病症状表现为:厌食、排脏、身体萎缩、体表局部溃烂乃至大面积溃烂。从患病刺参病灶处分离得到两种优势细菌CS1和CS2。经人工回接感染实验证明两种菌对健康刺参都具有较强的感染性,且感染病参的症状与自然发病刺参的症状相同。通过生理生化试验、16S rdnA序列分析及系统进化树分析,结果表明两株菌分别与灿烂弧菌(VIbrIO SPlEndIduS)和假交替单胞菌(PSEudOAlTErOMOnAS SP.)相似。菌株CS1与灿烂弧菌的亲缘关系最近,相似率达到99%,菌株CS2与假交替单胞菌的亲缘关系最近,相似率达到95%。菌株CS1可鉴定为灿烂弧菌,菌株CS2可鉴定为假交替单胞菌。另外,在患病刺参呼吸树膜和腔体内发现大量后口虫(bOVErIA SP.)。所以导致本次刺参“腐皮综合症“的原因可能是致病性细菌和寄生虫共同作用的结果。本文首次揭示了该地区“腐皮综合症“导致养成刺参大规模死亡的致病原因,对刺参病害防治和健康养殖具有重要的指导意义。In the winter of 2011,the sea cage cultured sea cucumber suffered from serious disease in many sea areas of Ningde in Fujian Province,accompanied by the phenomenon of death.The symptom displayed as anorexia,viscera ejection,general atrophy,and skin ulceration,the disease was tentatively termed as "skin ulcerative syndrome".The bacterium CS1 and CS2 were isolated from the focal area of the infected sea cucumber,and they were dominant amongst the isolates.Artificial infection test proved that they were the causative pathogen associated with the disease.In addition,a lot of Boveria sp.was found from the respiratory tree of sick sea cucumber.The bacterium CS1 and CS2 were identified as Vibrio splendidus and Pseudoalteromonas,respectively,by the means of morphological,biochemical tests,and 16S rDNA sequence analysis.This paper revealed for the first time that the causative pathogens leading to the "skin ulcerative syndrome" of the sea cucumber in the area,which will be helpful in the disease control and health management during sea cucumber cultivation.福建省科技厅项目(2010R1002-3

新工科背景下应用型本科院校的综合实践教学改革———以西安文理学院机械设计制造及其自动化专业为例

论文对新工科人才国家战略需求标准进行研读，通过分析目前西安文理学院机械专业在新工科背景下人才培养方案的缺失得知，现有的实践环节不能支撑社会对复合型人才的需求，提出了解决思路，并给了具体操作方法，通过“以赛促教、赛教结合”，形成“教学—竞赛—科研”三位一体的综合实践课程体系，最后通过《制造技术与数控加工》综合实训课程进行了实践，收到了良好的效果。</jats:p

塔里木河三源流区气候变化对径流量的影响

结合对近50年塔里木河源流区气象、水文资料的分析,探讨了过去半个世纪塔里木河源流区气候变化及其对河川径流的影响。研究结果显示,在过去50年里,塔里木河三源流径流流量总体呈现增加的趋势,期间有波动过程;对影响径流变化的气温、降水和蒸发等因子分析发现,降水量变化对塔里木河径流量变化影响最为显著,而温度的升高,加速了山区冰雪资源的消融,加大了冰雪融水对径流量的补给,但同时导致蒸发量增大,增加了地表淡水资源的消耗,对山区来水量的增大起到一定的削弱作用

GFP报告因子结合流式细胞术的蛋白-蛋白相互作用单细菌水平分析

在基于腺苷酸环化酶功能重构的细菌双杂交(BACTH)系统中引入lac启动子控制的gfp基因作为蛋白质相互作用的报告基因,采用实验室自行研制的超高灵敏流式检测装置(HSFCM)建立了一种单细菌水平的、简单、快速、高通量的蛋白-蛋白相互作用分析方法.将分别携带有相互作用蛋白对编码基因和gfp报告基因的质粒共转化至报告菌株,利用HSFCM对单个细菌的散射光和荧光同时进行检测.结果表明蛋白-蛋白相互作用激活了gfp报告基因的表达,并存在双稳态现象:即一部分细菌的gfp报告基因被激活,细菌荧光信号强度明显高于阴性对照组,而另一部分细菌的gfp报告基因未被激活,细菌荧光信号分布与阴性对照组重叠.利用HSFCM在单细菌水平对荧光信号强度和表达GFP报告因子的细菌比例进行分析,能有效地区分表达GFP报告因子的阳性菌和报告基因未被激活表达的阴性菌,揭示了细菌个体蛋白的异质性表达.这将为相互作用蛋白对的检测及筛选提供一种快速的分析方法.国家自然科学基金(21472158,21105082,21225523,21475112,216278111