13 research outputs found
Efficacy of contact lens solutions against bacteria ısolated from clinical specimens
Bu çalışmada, son yıllarda piyasada satışa sunulan iki adet çok amaçlı kontakt lens dezenfektan solüsyonunun klinik
örneklerden izole edilen Staphylococcus aureus Rosenbach ve Pseudomonas aeruginosa Gessard bakterilerine karşı etkinliği
araştırılmıştır. Çok amaçlı kontakt lens dezenfektan solüsyonlarının antibakteriyal etkinlikleri ISO 14729’da belirtilen kültüre
dayalı mikrobiyolojik yöntem kullanılarak belirlenmiştir. Üretici tarafından önerilen minimum dezenfeksiyon sürelerine ek
olarak belirlenen örnekleme saatlerindeki bakteri sayıları tespit edilmiş ve ortalama log düşüşleri hesaplanmıştır. Çalışmada
antibakteriyal etkinlikleri test edilen bu kontakt lens dezenfektan solüsyonlarının 3 farklı lotu kullanılmıştır. Elde edilen
sonuçlar, her iki kontakt lens dezenfektan solüsyonunun üretici tarafından önerilen minimum dezenfeksiyon süresi sonunda
denenen bakterilerin sayılarında en az 3 log düşüş sağladığını göstermiştir. Kontakt lens solüsyon endüstrisinde gelişen
teknolojiler ve yapılan bilimsel çalışmalar ile yeni formülasyonlar geliştirilmektedir. Piyasada satışa sunulan bu yeni ürünlerin
antibakteriyal aktivitelerinin tam olarak anlaşılabilmesi için farklı kaynaklardan izole edilen suşların kullanılacağı ileriki
çalışmalara ihtiyaç duyulmaktadır.In this study, the efficacy of two recently marketed multipurpose contact lens disinfecting solutions against
Staphylococcus aureus Rosenbach and Pseudomonas aeruginosa Gessard bacteria isolated from clinical specimens were
investigated. The antibacterial activities of multipurpose contact lens disinfecting solutions were determined by microbiological
culture method described in ISO 14729 guidelines. Bacterial counts were obtained for sampling intervals determined in addition
to the manufacturer’s minimum recommended disinfection time and mean log reductions were calculated for each interval.
Three different lots of each contact lens disinfecting solutions were used. The results showed that both contact lens disinfecting
solutions provided at least 3 log reductions for all bacteria tested at the end of the manufacturer’s minimum recommended
disinfection time. New formulations are being developed in contact lens disinfecting solution industry by new technologies and
scientific researches. Further studies on strains isolated from different sources are needed in order to a complete understanding
of these newly marketed contact lens solution
Investigation Of The Survival Term Of Legionella Pneumophila In Different Media And Temperatures
AMOEBICIDAL EFFICACY OF A NOVEL MULTI-PURPOSE DISINFECTING SOLUTION: FIRST FINDINGS
Free-living amoebae of the genus Acanthamoeba can cause a sight-threatening corneal infection, Acanthamoeba keratitis, mostly in contact lens wearers. The use of ineffective contact lens disinfecting solutions is one of the most important risk factors for this infection. This study concerns a new multi-purpose contact lens disinfecting solution, OPTI-FREE (R) PureMoist (R), tested for its efficacy against Acanthamoeba trophozoites and cysts by using the most probable number technique for amoebic enumeration. Acanthamoeba castellanii ATCC 50373 and an environmental strain of Acanthamoeba genotype T4 isolated from tap water in Istanbul were used during the experiments. OPTI-FREE (R) PureMoist (R) achieved total kill (more than a 3-log reduction) of trophozoites of both strains before the manufacturer-recommended disinfection time (6 h). In contrast, this solution had limited cysticidal activity against the ATCC strain but more against the environmental strain, with log reductions of 0.75 and 2.20, respectively, after 6 h of exposure. (C) 2014 Elsevier Inc. All rights reserved