Dose-dependant preventive effect of a herbal compound on crystal formation in rat model

Introduction: To analyze the dose-dependent preventive effect of a plant-based herbal product on the new crystal formation in a rat model. Materials and methods: A total of 42 rats were divided into 7 groups and zinc discs were placed into the bladder of rats to provide a nidus for the development of new crystal formation: Group 1: control, Group 2: 0.75 percent ethylene glycol (EG); Group 3: 0.75 percent EG plus 0.051 ml of the compound; Group 4: 0.75 percent EG plus 0.179 ml of the compound; Group 5: 0.75 percent EG plus 0.217 ml of the compound; Group 6: 0.75 percent EG plus 0.255 ml of the compound; Group 7 0.75 percent EG plus 0.332 of the compound). The analysis and comparison focused on the disc weights, changes in urinary oxalate and calcium levels, urinary pH, and the histopathologic evaluation of the inflammatory changes in the bladder after 14 days. Results: According to the evaluation of discs placed in the bladders of the animals, animals receiving the herbal compound on a dose-dependent basis showed a limited increase in the disc weights values after 14 days, despite a considerable increase in animals receiving EG alone (p = 0.001). Further evaluation of the increase in disc weights on a dose-dependent basis in different subgroups (from Groups 3 to 7) demonstrated that the limitation of crystal deposition began to be more prominent as the dose of herbal compound increased. This effect was more evident particularly in comparisons between group 7 and others, according to LSD multiple comparison tests (p = 0.001). As anticipated, there has been no discernible change in the weight of the discs in the control group. Although urinary calcium levels in animals of Groups 2, 6, and 7 were significantly higher than the other groups, we were not able to demonstrate a close correlation between urinary oxalate levels and the increasing dose levels. Even though mean urine pH levels were statistically considerably higher in Group 3, there was no statistically significant correlation between the oxalate and calcium levels between all groups, and no association was seen with the administration of herbal agents. The transitional epithelium between the three groups of animals' bladder samples did not exhibit any appreciable difference according to pathological analysis. Conclusions: In this animal model, the treatment of the compound was successful in lowering the amount of crystal deposition surrounding the zinc discs, most noticeably at a dosage of 0.332 ml, three times per day

Identification of Normal Retina's Variations in Kars Shepherd Dogs via Fundoscopic Examination

The purpose of this study was to use fundoscopic examination to identify normal variations in the retinas of Kars Shepherd Dogs, which are raised in northeastern Turkey, particularly in the province of Kars and the surrounding area. The study was carried out on 50 healthy Kars Shepherd Dogs of different ages and genders. The tapetal fundus was observed to be granular and green-yellow-blue in color, while the nontapetal fundus was observed to be dark colored (dark brown). No statistically significant difference was found between male and female (P > 0.05) for the existence of these granules in the tapetal region. The optic disc was found to be light pink in color and oval in shape with rounded corners. Although the disc was generally located in the tapetal region, it was also found to be located at the intersection of the tapetal and nontapetal regions. No statistically significant difference was found between the dogs regarding the location of the optical disc with respect to the age or gender of the dogs (P > 0.05). In conclusion, Kars Shepherd Dogs were found to have a granular, green-yellow-blue tapetum, a dark colored non-tapetum, and an optic disc that was light pink in color and oval in shape with rounded corners; therefore we arrived at the conclusion that the color and variations that we found were breed-specific, as were the other morphological characteristics

Fluorescein as a diagnostic marker of bladder ruptures: an experimental study on rabbit model

Introduction: The aim of this study was to investigate fluorescein use in the diagnosis of bladder ruptures in rabbits as an experimental model