Design and preparation of functional nanoparticles and their application in cancer diagnosis and therapy

功能化无机纳米材料具有良好的生物兼容性、优异理化性质、易于表面修饰以及尺寸、形貌易于调控等优势，被广泛应用于生物医学领域。其中，磁共振成像造影剂和抗肿瘤药物运载系统，以其能有效提高肿瘤诊断的准确性和治疗的效果，已经成为该域中最富有活力的分支，受到广大科研工作者的关注。鉴于发展新型高效的磁共振造影剂和无机纳米抗肿瘤药物运载系统具有重要的现实意义，本博士论文设计合成了多种功能性无机纳米材料，并对其肿瘤诊断和治疗的能力进行了系统性的考察。主要研究内容概括如下： 第一章：简要综述了功能化无机纳米材料在磁共成像振造影剂和抗肿瘤药物运载系统方面的研究进展，并阐明博士论文的选题依据和研究内容。 第二章：...Functional inorganic nanomaterials with good biocompatible, excellent physical and chemical properties, easy modification, and tunable size and morphology have been widely used in biomedical. Since magnetic resonance imaging (MRI) contrast agent and inorganic drug delivery systems can improve the accuracy and efficiency in cancer diagnosis and therapy, development of new and effient inorganic nano...学位：理学博士院系专业：化学化工学院_化学生物学学号：2052012015347

Quantitative Analysis of Surface-Immobilized Hydrazide Groups by Spectrophotometry

建立了一种测定固相表面酰肼基团含量的方法。运用fE(Ⅲ)-邻二氮菲显色体系,通过固相表面具有较强还原性的酰肼基团将fE(Ⅲ)还原为fE(Ⅱ),生成的fE(Ⅱ)可与邻二氮菲生成稳定的桔红色络合物,从而利用分光光度法测定溶液体系吸光度来表征固相表面酰肼基团的含量。该方法在0~0.02μMOl/Ml范围内呈良好线性关系,线性相关系数r=0.9975,检出限为5.4x10-4μMOl/Ml。用该方法测出微珠固相表面酰肼基团的活化效率为72%。方法可以很好地应用于固相载体表面酰肼基团的测定。A measurement approach of hydrazide groups on solid-phase surface by spectrophotometry was reported.By reducing Fe(Ⅲ) to Fe(Ⅱ) in solutions with microbead surface-conjugate hydrazide groups,a stable orange-red complex of Fe(Ⅱ)-orthophenanthroline was formed.The content of hydrazide groups on the bead surface was then characterized by spectrophotometric measurement.This method has advantages of high sensitivity,simplicity and practicability.The linear range is from 0 to 0.02 μmol/mL with the linear correlation coefficient of 0.9975 and detection limit of 5.4×10-4 μmol/mL(S/N=3).In this method,72% of the activation efficiency of the hydrazide groups activated on the carboxylated microbead surface was detected.It is promising to apply the approach to the quantitative assessment of hydrazide groups on various solid-phase surfaces.国家自然科学基金(No.20775065;No.20835005);教育部高校博士点基金(No.20070384023);国家基础科学人才培养基金(No.J1030415