Synthesis and Characterization of Functional Polymer Microgels

高分子凝胶是一种三维交联网络结构的高分子材料，具有多孔性、高稳定性和易功能化等特性。与宏观凝胶相比，高分子微凝胶表现出更快的传输速度和响应性能，在传感、催化、药物释放等领域有潜在的应用。因此，高分子微凝胶的合成及功能化一直是人们研究的热点。 众所周知，对一些挥发性有机小分子，尤其是生物标记分子的识别和检测，在环境监测、人体代谢及疾病诊断等领域具有非常重要的意义。其中，氨气是肾衰竭、螺旋杆菌和口腔疾病的一种生物标记物，其检测方法主要存在稳定性差，难以循环使用等缺点。丙酮与肺癌、糖尿病、充血性心力衰竭、脑疾病等有关，其检测方法主要局限于色谱法。因此，发展简单、高效的分子识别检测体系仍是非常有挑战...Polymer gels are three-dimensional crosslinked polymer networks which posses the properties of porosity, stability and easy functionalization etc. Compared with macrogels, microgels have faster transmission and responsiveness, having a wide range of prospective applications in sensing, catalysis, drug release, and other fields. Hence, synthesis and functionalization of microgels has been a hot top...学位：理学硕士院系专业：化学化工学院_高分子化学与物理学号：2052013115166

The Optimization of Primary Hemocyte Culture of Scylla paramamosain

本研究通过对拟穴青蟹血细胞原代培养条件的摸索与优化,成功在体外培养了青蟹血细胞。结果表明,PH 4.6的海洋甲壳类血细胞抗凝剂培养青蟹血细胞的形态优于海洋蟹类抗凝剂(ACd);高血清浓度(>15%)培养血细胞形态较差,易出现空泡;添加5%fbS与1.2%nACl的l15培养基是培养拟穴青蟹血细胞最适培养基,并可在培养的青蟹原代血细胞中检测到稳定的管家基因表达,表明该细胞可用于拟穴青蟹体外功能试验研究。The optimization of primary hemocyte culture of Scylla paramamosain was successfully studied in vitro.The results showed that the hemocyte cells were morphologically kept activity better at pH 4.6marine crustaceans anticoagulant.High concentration of fetal bovine serum(>15%)in the culture lead to development of vacuoles.The hemocyte cells survived best in L15 medium supplemented with 5%fetal bovine serum and 1.2%sodium chloride.The house keeping gene β-actin of Scylla paramamosain of cultured hemocyte maintained stably expressed through the experiment.This cell culture system could be used for in vitro experiments.福建省农业科技重点项目(2012N0029); 中国博士后科学基金面上资助(2014M550354