Appendix A. Sample sites in Twelvemile Creek.

Sample sites in Twelvemile Creek

Index Files

Index File

Appendix C. Targeted PCB analytes and methods for extraction and analysis of samples by GC-ECD.

Targeted PCB analytes and methods for extraction and analysis of samples by GC-ECD

Appendix B. Summary PCB, 15N, and 13C for stream and riparian food webs.

Summary PCB, 15N, and 13C for stream and riparian food webs

Appendix A. Location of study area and sampling sites.

Location of study area and sampling sites

Appendix B. Lake Hartwell water elevation 1962–2007.

Lake Hartwell water elevation 1962–2007

Box and whisker plots of nitrate uptake velocity (ʋ_f) in the buried and open reaches in Cincinnati, Ohio and Baltimore, Maryland, as reported in Beaulieu et al. [20] and Pennino et al. [21].

<p>Literature data were derived from a recent survey of 72 streams spanning several biomes and land-use conditions [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132256#pone.0132256.ref016" target="_blank">16</a>]. Plots display 10^th, 25^th, 50^th, 75^th, and 90^th percentiles and individual data points outside the 10^th and 90^th percentiles. Nitrate uptake velocity was 13 times greater in open than buried reaches (p<0.001, paired <i>t</i>-test).</p

Results of simulation scenarios involving an even distribution of burial across the watershed with incremental increases of 5%.

<p>The primary y-axis and solid line represent the average volumetric NO₃^- uptake rate among in the open reaches. The secondary y-axis and dashed line represent total NO₃^- uptake in the open reaches.</p

Stream burial is an extreme, but ubiquitous, consequence of urbanization in stream ecosystems.

<p>The buried stream channels in the cited studies were constructed from various materials including (a) a cement-lined corrugated metal pipe in Baltimore, Maryland (USA), (b) a concrete tunnel in Cincinnati, Ohio (USA), and (c) a corrugated metal pipe in Cincinnati.</p

Percent change in nitrate export in response to stream burial simulation scenarios.

<p>The simulation scenarios involve an even distribution of burial across the watershed with incremental increases of 5% and include: 1) Allowing both uptake rate constants and water velocities to change in response to burial (Combined response); 2) Allowing water velocity to change following burial, but holding uptake rate constants at open reach values; and 3) Allowing uptake rate constants to change following burial, but holding water velocities at open reach values.</p