PALLADIUM BASED CONTACTS TO GaAs AND InP

Au(85nm)/Pd(55nm) and Pd(55nm) metallizations were deposited on GaAs(lOO) and InP(100) substrates. The samples were heat treated in a scanning electron microscope (SEM) equipped with a quadrupole mass spectrometer. The simultaneous observation of the volatile component loss (in situ) by Evolved Gas Analysis (EGA) and the change in surface morphology by SEM during the heart treatment using a heating rate of 30°C/min were carried out. The interaction of the metallization with compound semiconductor substrates was observed after the heat treatment by transmission electron microscopy (TEM) using samples prepared by cross-sectional technique. In the course of the present work a large volatile component (arsenic and phosphorus) loss was observed for the samples coated by single layer metallization (at 410° C for Pd/GaAs and at 580°C for Pd/InP) and two peaks were registered in the case of Au/Pd metallization. In the latter case the cause of the second evaporation peak is the interaction between diffused gold and compound semiconductor. The SEM images of the surfaces demonstrate a significant change of the surface morphology at the singularities of the EGA curves. The grains grown into the semiconductors are shown by the cross-sectional images of the heat treated samples

Comparison of gene expression signatures of diamide, H(2)O(2 )and menadione exposed Aspergillus nidulans cultures – linking genome-wide transcriptional changes to cellular physiology

BACKGROUND: In addition to their cytotoxic nature, reactive oxygen species (ROS) are also signal molecules in diverse cellular processes in eukaryotic organisms. Linking genome-wide transcriptional changes to cellular physiology in oxidative stress-exposed Aspergillus nidulans cultures provides the opportunity to estimate the sizes of peroxide (O(2)(2-)), superoxide (O(2)(•-)) and glutathione/glutathione disulphide (GSH/GSSG) redox imbalance responses. RESULTS: Genome-wide transcriptional changes triggered by diamide, H(2)O(2 )and menadione in A. nidulans vegetative tissues were recorded using DNA microarrays containing 3533 unique PCR-amplified probes. Evaluation of LOESS-normalized data indicated that 2499 gene probes were affected by at least one stress-inducing agent. The stress induced by diamide and H(2)O(2 )were pulse-like, with recovery after 1 h exposure time while no recovery was observed with menadione. The distribution of stress-responsive gene probes among major physiological functional categories was approximately the same for each agent. The gene group sizes solely responsive to changes in intracellular O(2)(2-), O(2)(•- )concentrations or to GSH/GSSG redox imbalance were estimated at 7.7, 32.6 and 13.0 %, respectively. Gene groups responsive to diamide, H(2)O(2 )and menadione treatments and gene groups influenced by GSH/GSSG, O(2)(2- )and O(2)(•- )were only partly overlapping with distinct enrichment profiles within functional categories. Changes in the GSH/GSSG redox state influenced expression of genes coding for PBS2 like MAPK kinase homologue, PSK2 kinase homologue, AtfA transcription factor, and many elements of ubiquitin tagging, cell division cycle regulators, translation machinery proteins, defense and stress proteins, transport proteins as well as many enzymes of the primary and secondary metabolisms. Meanwhile, a separate set of genes encoding transport proteins, CpcA and JlbA amino acid starvation-responsive transcription factors, and some elements of sexual development and sporulation was ROS responsive. CONCLUSION: The existence of separate O(2)(2-), O(2)(•- )and GSH/GSSG responsive gene groups in a eukaryotic genome has been demonstrated. Oxidant-triggered, genome-wide transcriptional changes should be analyzed considering changes in oxidative stress-responsive physiological conditions and not correlating them directly to the chemistry and concentrations of the oxidative stress-inducing agent

RNA-DNA hybrid (R-loop) immunoprecipitation mapping: an analytical workflow to evaluate inherent biases

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Biophysical characterization of histone H3.3 K27M point mutation

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Coding and Noncoding Transcriptomes of NODULIN HOMEOBOX (NDX)-Deficient Arabidopsis Inflorescence

Arabidopsis NODULIN HOMEOBOX (NDX) is a plant-specific transcriptional regulator whose role in small RNA biogenesis and heterochromatin homeostasis has recently been described. Here we extend our previous transcriptomic analysis to the flowering stage of development. We performed mRNA-seq and small RNA-seq measurements on inflorescence samples of wild-type and ndx1-4 mutant (WiscDsLox344A04) Arabidopsis plants. We identified specific groups of differentially expressed genes and noncoding heterochromatic siRNA (hetsiRNA) loci/regions whose transcriptional activity was significantly changed in the absence of NDX. In addition, data obtained from inflorescence were compared with seedling transcriptomics data, which revealed development-specific changes in gene expression profiles. Overall, we provide a comprehensive data source on the coding and noncoding transcriptomes of NDX-deficient Arabidopsis flowers to serve as a basis for further research on NDX function