2 research outputs found

    Doenças do trato urinário em cães e gatos: um estudo retrospetivo da prescrição e resistência aos antibióticos

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    Orienta√ß√£o: Jo√£o Manuel Cardoso MartinsAs doen√ßas do trato urin√°rio em c√£es e gatos representam uma boa parte dos atendimentos cl√≠nicos. As infe√ß√Ķes urin√°rias s√£o parte significante na prescri√ß√£o de antibi√≥ticos, por vezes desnecess√°rios, contribuindo para aumentar a resist√™ncia bacteriana. Para descrever a epidemiologia local de infe√ß√Ķes urin√°rias e as suas apresenta√ß√Ķes, foram analisadas as fichas cl√≠nicas de 37 animais, sendo 23 c√£es e 15 gatos, ap√≥s as amostras de urina serem submetidas a cultivo aer√≥bio, para classificar as doen√ßas do trato urin√°rio quando presentes, em rela√ß√£o √† forma cl√≠nica, microrganismos isolados, perfil de resist√™ncia e tratamento. Foi identificado no isolamento bacteriano em 11 c√£es, sendo que 4 apresentaram cistite bacteriana espor√°dica, 6 com recorrente e 1 em que n√£o foi poss√≠vel a classifica√ß√£o. Foram isolados: E. coli, Staphylococcus spp., Enterobacter aerogenes e Proteus spp., com perfil fenot√≠pico de resist√™ncia predominante AmpC (Ambler). Nos gatos, o isolamento foi obtido em 4, sendo 3 cistites espor√°dicas, 1 recorrente e 4 cistites idiop√°ticas felinas, todos com E. coli, com resist√™ncia AmpC em 1 amostra, 2 sem resist√™ncia aos antibi√≥ticos testados e 1 resistente a enrofloxacina e cefalotina. A correla√ß√£o de pi√ļria com o isolamento bacteriano foi inconsistente nos gatos e consistente nos c√£es; a de altera√ß√Ķes ou doen√ßas concomitantes foi inconsistente para ambos.Dogs‚Äô and cats‚Äô urinary tract diseases represent most of clinical treatments. Urinary infections are a significant part on antibiotics‚Äô prescription, sometimes unnecessary, helping to increase the bacteria‚Äôs resistance. In order to describe the local epidemiology of urinary infections and its presentations, thirty-seven animals, including twenty-three dogs and fifteen cats, had their clinical files analyzed after urine samples were submitted to aerobic culture in order to classify urinary tract diseases if present, in relation to the clinical form, isolated microorganisms, resistance profile, and treatment. Bacterial isolation was identified in eleven dogs, four of them with sporadic bacterial cystitis, six recurrent bacterial cystitis, and in one of the dogs it was not possible to classify bacterial cystitis. The isolated microorganisms were E. coli, Staphylococcus spp., Enterobacter aerogenes and Proteus spp. The predominant phenotypic profile of resistance was AmpC, according to the Ambler classification. Bacterial isolation was obtained in four cats, three sporadic cystitis, one recurrent cystitis and four idiopathic feline cystitis, all E. coli with phenotypic profile of AmpC resistance in one sample, two with no resistance to the tested antibiotics and one with resistance to enrofloxacin and cephalothin. The correlation of the presence of pyuria with bacterial isolation was inconsistent in cats and consistent in dogs. The correlation of concomitant changes or diseases was inconsistent for both cats and dogs

    Tinea capitis : an√°lise em hospital universit√°rio terci√°rio da √°rea de Lisboa (Agosto de 2018 a Maio de 2020)

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    Tese de mestrado, Microbiologia Cl√≠nica e Doen√ßas Infecciosas Emergentes, Universidade de Lisboa, Faculdade de Medicina, 2021A tinea capitis √© uma infe√ß√£o dermatof√≠tica do couro cabeludo, que acomete principalmente crian√ßas entre tr√™s e oito anos e tem impacto social relevante. A les√£o prim√°ria pode evoluir para alopecia cicatricial como sequela. A presente tese teve com objetivo relatar as esp√©cies de tinea capitis prevalentes na regi√£o de Lisboa, identificar a faixa et√°ria mais atingida, comparar estatisticamente os resultados entre os principais meios de diagn√≥stico (exame direto e cultivo micol√≥gico), otimizar o diagn√≥stico definitivo com a associa√ß√£o de exames de rea√ß√£o em cadeia da polimerase (PCR) e sequencia√ß√£o de amostras obtidas de col√≥nias de fungos dermat√≥fitos. Foram estudadas 248 amostras cl√≠nicas com diagn√≥stico presuntivo de tinea capitis de doentes atendidos no servi√ßo de Dermatologia do Hospital de Santa Maria (um hospital universit√°rio terci√°rio), Lisboa, no per√≠odo entre agosto de 2018 e maio de 2020. Das amostras analisadas, 111 foram positivas, somando-se os resultados dos exames direto e de cultura. Foram identificados dermat√≥fitos no cultivo micol√≥gico em 102 amostras: 48 casos de Microsporum audouinii (47,06%), 37 de Trichophyton soudanense (36,28%), 9 de Trichophyton tonsurans (8,82%), 2 de Trichophyton mentagrophytes (1,96%), 1 de Microsporum canis (0,98%), 3 em que n√£o foi poss√≠vel diferenciar Microsporum audouinii de Microsporum canis (2,94%) e 2 em que n√£o foi poss√≠vel a diferencia√ß√£o entre Trichophyton soudanense e Trichophyton violaceum (1,96%). Dos exames positivos, 33% dos doentes eram declarados como pertencentes ao fototipo V ou VI. A identifica√ß√£o de dermat√≥fitos por PCR-sequencia√ß√£o esteve em concord√Ęncia significativa com a identifica√ß√£o microsc√≥pica das col√īnias cultivadas.Tinea capitis is a dermatophytic infection of the scalp, which mainly affects children aged between three and eight years and has a relevant social impact. The primary lesion may progress to cicatricial alopecia as a sequel. This study aimed at reporting the species of tinea capitis in the Lisbon region, describing age and sex of affected patients, comparing statistically the results between the main means of diagnosis (direct examination and mycological cultivation), and optimizing the diagnosis with the association of polymerase chain reaction (PCR) followed by sequencing assays performed on clinical samples and fungal colonies. A total of 248 clinical samples with a presumptive diagnosis of tinea capitis were studied, from patients treated at the Dermatology Department of Hospital de Santa Maria (a tertiary, referral and teaching Hospital), Lisboa, between August 2018 and May 2020. A total of 111 tests were considered positive, comprising the results of both direct examinations of clinical samples plus the macroscopic and microscopic examination of fungal cultures. Dermatophytes were identified in the mycological culture in 102 cases: 48 cases of Microsporum audouinii (47.04%), 37 of Trichophyton soudanense (33.34%), 9 of Trichophyton tonsurans (8.82%), 2 of Trichophyton mentagrophytes (1.96%), and 1 of Microsporum canis (0.98%). In 3 and 2 cases, respectively, it was not possible to differentiate between Microsporum audouinii and Microsporum canis (2.94%), and between Trichophyton soudanense and Trichophyton violaceum (1.96%). Of the positive cases, 33% referred to patients declared to belong to phototype V or VI. The identification of dermatophytes by PCR-sequencing were in excellent agreement with their identification based on microscopic examination of fungal colonies
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