Evolutionary constraints on the complexity of genetic regulatory networks allow predictions of the total number of genetic interactions

Genetic regulatory networks (GRNs) have been widely studied, yet there is a lack of understanding with regards to the final size and properties of these networks, mainly due to no network currently being complete. In this study, we analyzed the distribution of GRN structural properties across a large set of distinct prokaryotic organisms and found a set of constrained characteristics such as network density and number of regulators. Our results allowed us to estimate the number of interactions that complete networks would have, a valuable insight that could aid in the daunting task of network curation, prediction, and validation. Using state-of-the-art statistical approaches, we also provided new evidence to settle a previously stated controversy that raised the possibility of complete biological networks being random and therefore attributing the observed scale-free properties to an artifact emerging from the sampling process during network discovery. Furthermore, we identified a set of properties that enabled us to assess the consistency of the connectivity distribution for various GRNs against different alternative statistical distributions. Our results favor the hypothesis that highly connected nodes (hubs) are not a consequence of network incompleteness. Finally, an interaction coverage computed for the GRNs as a proxy for completeness revealed that high-throughput based reconstructions of GRNs could yield biased networks with a low average clustering coefficient, showing that classical targeted discovery of interactions is still needed.Comment: 28 pages, 5 figures, 12 pages supplementary informatio

El entrenamiento de la resistencia de la fuerza rápida en luchadores pioneriles

The present work aims to elaborate a methodological alternative for the development of fast force resistance in wrestlers of the pioneer category of the sports team “Jesús Feliu Leyva” of Holguín municipality in 2017. To carry out the investigation, methods from the theoretical and empirical level methods were used, that made possible to understand the different elements that affect the problem under study, as well as the possibility of finding an adequate scientific answer to these elements.El presente trabajo tiene como objetivo elaborar una alternativa metodológica para el desarrollo de la resistencia de la fuerza rápida en luchadores de la categoría pioneril del combinado deportivo “Jesús Feliu Leyva” del municipio Holguín. Para el desarrollo de la investigación se utilizaron métodos del nivel teórico y empírico, que posibilitaron la compresión de los distintos elementos que inciden en el problema objeto de estudio, así como la posibilidad de encontrar una respuesta científica adecuada a estos elementos

Corynebacterium glutamicum regulation beyond transcription: Organizing principles and reconstruction of an extended regulatory network incorporating regulations mediated by small RNA and protein-protein interactions

Corynebacterium glutamicum is a Gram-positive bacterium found in soil where the condition changes demand plasticity of the regulatory machinery. The study of such machinery at the global scale has been challenged by the lack of data integration. Here, we report three regulatory network models for C. glutamicum: strong (3040 interactions) constructed solely with regulations previously supported by directed experiments; all evidence (4665 interactions) containing the strong network, regulations previously supported by non-directed experiments, and protein-protein interactions with a direct effect on gene transcription; and sRNA (5222 interactions) containing the all evidence network and sRNA-mediated regulations. Compared to the previous version (2018), the strong and all evidence networks increased by 75 and 1225 interactions, respectively. We analyzed the system-level components of the three networks to identify how they differ and compared their structures against those for the networks of more than 40 species. The inclusion of the sRNAs regulations changed the proportions of the system-level components and increased the number of modules but decreased their size. The C. glutamicum regulatory structure contrasted with other bacterial regulatory networks. Finally, we used the strong networks of three model organisms to provide insights and future directions of the C. glutamicum regulatory network characterization.Comment: 32 pages, 4 figures, 1 supplementary materia

Reflexiones sobre la concepción teórico-metodológica como resultado científico: Una mirada desde el balonmano (Original

In the current article you carries out an appreciation on the conception term from the scientific investigation, tried by different authors, from different positions according to the area of knowledge, where it is defined as theoretical, methodological, didactics, educational, or theoretical-methodological. Certain coincidence was appreciated in the definitions, it structures and the purpose that should be defined in the practice, for what understood each other that the conception is a theoretical sustenance and it is materialized, by means of systems, methodologies, strategies and other practical results obtained during the training from the handball porteras to school level. En el presente artículo se llevó a cabo una conceptualización del término concepción desde la investigación científica, tema tratado por diferentes autores desde posiciones diversas y según el área de conocimiento de cada uno. La concepción fue caracterizada como teórica, metodológica, didáctica, educativa o teórico-metodológica y aunque se apreció cierta coincidencia tanto en las definiciones consultadas como en la estructura y en la finalidad del término, se estableció que el concepto debe definirse en la práctica, pues se entendió que la concepción es un sustento teórico que se materializa durante el entrenamiento de las porteras de balonmano a nivel escolar mediante sistemas, metodologías, estrategias y otros resultados prácticos

Abasy Atlas: a comprehensive inventory of systems, global network properties and systems-level elements across bacteria

Ibarra-Arellano MA, Campos-Gonzalez AI, Trevino-Quintanilla LG, Tauch A, Freyre-Gonzalez JA. Abasy Atlas: a comprehensive inventory of systems, global network properties and systems-level elements across bacteria. DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION. 2016;2016: baw089.The availability of databases electronically encoding curated regulatory networks and of high-throughput technologies and methods to discover regulatory interactions provides an invaluable source of data to understand the principles underpinning the organization and evolution of these networks responsible for cellular regulation. Nevertheless, data on these sources never goes beyond the regulon level despite the fact that regulatory networks are complex hierarchical-modular structures still challenging our understanding. This brings the necessity for an inventory of systems across a large range of organisms, a key step to rendering feasible comparative systems biology approaches. In this work, we take the first step towards a global understanding of the regulatory networks organization by making a cartography of the functional architectures of diverse bacteria. Abasy (Across-bacteria systems) Atlas provides a comprehensive inventory of annotated functional systems, global network properties and systems-level elements (global regulators, modular genes shaping functional systems, basal machinery genes and intermodular genes) predicted by the natural decomposition approach for reconstructed and meta-curated regulatory networks across a large range of bacteria, including pathogenically and biotechnologically relevant organisms. The meta-curation of regulatory datasets provides the most complete and reliable set of regulatory interactions currently available, which can even be projected into subsets by considering the force or weight of evidence supporting them or the systems that they belong to. Besides, Abasy Atlas provides data enabling large-scale comparative systems biology studies aimed at understanding the common principles and particular lifestyle adaptions of systems across bacteria. Abasy Atlas contains systems and system-level elements for 50 regulatory networks comprising 78 649 regulatory interactions covering 42 bacteria in nine taxa, containing 3708 regulons and 1776 systems. All this brings together a large corpus of data that will surely inspire studies to generate hypothesis regarding the principles governing the evolution and organization of systems and the functional architectures controlling them

Functional architecture of Escherichia coli: new insights provided by a natural decomposition approach

The E. coli transcriptional regulatory network is shown to have a nonpyramidal architecture of independent modules governed by transcription factors, whose responses are integrated by intermodular genes

Partition Quantitative Assessment (PQA): A quantitative methodology to assess the embedded noise in clustered omics and systems biology data

Identifying groups that share common features among datasets through clustering analysis is a typical problem in many fields of science, particularly in post-omics and systems biology research. In respect of this, quantifying how a measure can cluster or organize intrinsic groups is important since currently there is no statistical evaluation of how ordered is, or how much noise is embedded in the resulting clustered vector. Many of the literature focuses on how well the clustering algorithm orders the data, with several measures regarding external and internal statistical measures; but none measure has been developed to statistically quantify the noise in an arranged vector posterior a clustering algorithm, i.e., how much of the clustering is due to randomness. Here, we present a quantitative methodology, based on autocorrelation, to assess this problem.Comment: 9 pages, 6 figure

Gestión del conocimiento para el desarrollo local de comunidades costeras en condiciones de fragilidad ambiental. Isabela de Sagua, Cuba.

Knowledge plays an essential place for development in the contemporary world due to its exponential growth and its impact on social life. Its management has become a matter of the first order because of the need to promote its use in the most diverse social processes, from the most relevant at the macro level to those of daily life. In this the universities have an assignment that goes beyond the training of professionals to be institutions that lead the production, dissemination and use of knowledge.In Cuba, knowledge management is an extended practice as a contribution to local community development processes, along with the dissemination of Municipal University Centers with the capacity to promote the continuity of learning in all sectors of society and for scientific production with high rigor for the solution of local problems.El conocimiento desempeña un lugar esencial para el desarrollo en el mundo contemporáneo por su crecimiento exponencial y por su incidencia en la vida social. Su gestión ha devenido una cuestión de primer orden por la necesidad de propiciar su empleo en los más diversos procesos sociales, desde los más relevantes a nivel macro hasta los de vida cotidiana. En ello las universidades tienen un encargo que va más allá de la formación de profesionales al ser instituciones que lideran la producción, difusión y uso del conocimiento.En Cuba la gestión el conocimiento es una práctica extendida como contribución a los procesos de desarrollo local comunitario, junto con la difusión de Centros Universitarios Municipales con capacidad para propiciar la continuidad del aprendizaje en todos los sectores de la sociedad y para la producción científica con alto rigor para la solución de problemáticas locales