1,870 research outputs found
Redefining Transitional Housing For Homeless Families
The future directions of policies designed to aid homeless families should be based on a deep understanding of the causes of homelessness. Unfortunately, systematic data are lacking. Instead, biases and opinions have shaped the debate about the origins of homelessness and its solutions. Some experts argue that widespread construction of low-income housing will solve the homelessness problem. Attractive as such a proposal might be, it overlooks the contribution of noneconomic factors to homelessness, as well as the quality of life of the families involved. The provision of decent, affordable housing is an essential element of a comprehensive plan; but such a plan must also address the social, emotional, medical, and educational needs of homeless parents and children
Homelessness Past and Present: The Case of the United States, 1890-1925
An examination of the professional, political, and popular literature on the nature and extent of homelessness from 1890 to 1925 affords a comparison of the economic and social characteristics of the homeless population at the turn of the century with that of today. The discussion covers the ensuing debates over the causes of homelessness, the various subgroups among the homeless during both periods, and the relative rates of homelessness, the context of extreme poverty and dislocation, and the prevalence of individual disabilities. Except for the growing numbers of homeless families over the past decade, the homeless populations during both eras have many similarities. Then and now, homeless people tend to be young, single, and America-born, with fragmented social supports and a history of dysfunctional family relationships. Although individual difficulties play an important role in determining who is most vulnerable, the authors argue that systemic ills plaguing society virtually ensure the existence of homelessness. Furthermore, during both eras ideologically driven views and moral prejudices have obscured the fundamental question of this country\u27s willingness to care for its neediest members
Age modification of the relationship between C-reactive protein and fatigue: findings from Understanding Society (UKHLS)
Background: Systemic inflammation may play a role in the development of idiopathic fatigue, that is, fatigue not explained by infections or diagnosed chronic illness, but this relationship has never been investigated in community studies including the entire adult age span. We examine the association of the inflammatory marker C-reactive protein (CRP) and fatigue assessed annually in a 3-year outcome period for UK adults aged 16–98. Methods: Multilevel models were used to track fatigue 7, 19, and 31 months after CRP measurement, in 10 606 UK individuals. Models accounted for baseline fatigue, demographics, health conditions diagnosed at baseline and during follow-up, adiposity, and psychological distress. Sensitivity analyses considered factors including smoking, sub-clinical disease (blood pressure, anaemia, glycated haemoglobin), medications, ethnicity, and alcohol consumption. Results: Fatigue and CRP increased with age, and women had higher values than men. CRP was associated with future self-reported fatigue, but only for the oldest participants. Thus, in those aged 61–98 years, high CRP ( > 3 mg/L) independently predicted greater fatigue 7, 19, and 31 months after CRP measurement [odds ratio for new-onset fatigue after 7 months: 1.88, 95% confidence interval (CI) 1.21–2.92; 19 months: 2.25, CI 1.46–3.49; 31 months: 1.65, CI 1.07–2.54]. No significant longitudinal associations were seen for younger participants. Conclusions: Our findings support previously described CRP–fatigue associations in older individuals. However, there are clear age modifications in these associations, which may reflect a contribution of unmeasured sub-clinical disease of limited relevance to younger individuals. Further work is necessary to clarify intervening processes linking CRP and fatigue in older individuals
Characterization of Two Distinct Calcium-Binding Sites in the Amino-Terminus of Human Profilaggrin
Profilaggrin is a large phosphorylated protein (approximately 400 kDa In humans) that is expressed in the granular cells of epidermis where it forms a major component of keratohyalin. It consists of multiple copies of similar filaggrin units plus amino- and carboxy-terminal domains that differ from filaggrin. Proteolytic processing of profilaggrin during terminal differentiation results in the removal of these domains and generation of monomeric filaggrin units, which associate with keratin intermediate filaments to form macrofibrils in the stratum corneum. The amino-terminal domain contains two calcium-binding motifs similar to the EF-hands found in the S-100 family of calcium-binding proteins. In this report, we expressed the 293-residue amino-terminal pro-domain of human profilaggrin as a polyhistidine fusion protein in Escherichia coli, and characterized calcium binding by a 45Ca++ binding assay and fluorescence emission spectroscopy. fluorescence measurements indicated that the profilaggrin polypeptide undergoes conformational changes upon the removal of Ca++ with ethylenediamine tetraacetic acid, demonstrating the presence of two calcium-binding sites with affinities for calcium that differ ninefold (1.4 Ă— 10-4 M and 1.2 Ă— 10-3 M). We suggest that this functional calcium-binding domain at the amino-terminus of human profilaggrin plays a role in profilaggrin processing and in other calcium-dependent processes during terminal differentiation of the epidermis
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Precision Medicine: Genetic Repair of Retinitis Pigmentosa in PatientDerived Stem Cells
Induced pluripotent stem cells (iPSCs) generated from patient fibroblasts could potentially be used as a source of autologous cells for transplantation in retinal disease. Patient-derived iPSCs, however, would still harbor disease-causing mutations. To generate healthy patient-derived cells, mutations might be repaired with new gene-editing technology based on the bacterial system of clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9, thereby yielding grafts that require no patient immunosuppression. We tested whether CRISPR/Cas9 could be used in patient-specific iPSCs to precisely repair an RPGR point mutation that causes X-linked retinitis pigmentosa (XLRP). Fibroblasts cultured from a skin-punch biopsy of an XLRP patient were transduced to produce iPSCs carrying the patient’s c.3070G>T mutation. The iPSCs were transduced with CRISPR guide RNAs, Cas9 endonuclease, and a donor homology template. Despite the gene’s repetitive and GC-rich sequences, 13% of RPGR gene copies showed mutation correction and conversion to the wild-type allele. This is the first report using CRISPR to correct a pathogenic mutation in iPSCs derived from a patient with photoreceptor degeneration. This important proof-of-concept finding supports the development of personalized iPSC-based transplantation therapies for retinal disease
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