104 research outputs found
Aspergillus strain typing in the genomics era
Multiple reasons may justify a need for strain typing purposes, but the
most common reason is to delineate the epidemiological relationships between
isolates. The availability of whole genome sequences has greatly influenced
our ability to develop highly targeted and efficient strain typing methods fur
these purposes. Some strain typing methods may serve dual goals: not only can
they be used to discriminate between multiple isolates of a certain species,
they can also aid in the recognition, identification, description and
validation process of a fungal species
High Resolution Genotyping of Clinical Aspergillus flavus Isolates from India Using Microsatellites
Contains fulltext :
124312.pdf (publisher's version ) (Open Access)BACKGROUND: Worldwide, Aspergillus flavus is the second leading cause of allergic, invasive and colonizing fungal diseases in humans. However, it is the most common species causing fungal rhinosinusitis and eye infections in tropical countries. Despite the growing challenges due to A. flavus, the molecular epidemiology of this fungus has not been well studied. We evaluated the use of microsatellites for high resolution genotyping of A. flavus from India and a possible connection between clinical presentation and genotype of the involved isolate. METHODOLOGY/PRINCIPAL FINDINGS: A panel of nine microsatellite markers were selected from the genome of A. flavus NRRL 3357. These markers were used to type 162 clinical isolates of A. flavus. All nine markers proved to be polymorphic displaying up to 33 alleles per marker. Thirteen isolates proved to be a mixture of different genotypes. Among the 149 pure isolates, 124 different genotypes could be recognized. The discriminatory power (D) for the individual markers ranged from 0.657 to 0.954. The D value of the panel of nine markers combined was 0.997. The multiplex multicolor approach was instrumental in rapid typing of a large number of isolates. There was no correlation between genotype and the clinical presentation of the infection. CONCLUSIONS/SIGNIFICANCE: There is a large genotypic diversity in clinical A. flavus isolates from India. The presence of more than one genotype in clinical samples illustrates the possibility that persons may be colonized by multiple genotypes and that any isolate from a clinical specimen is not necessarily the one actually causing infection. Microsatellites are excellent typing targets for discriminating between A. flavus isolates from various origins
Microsatellite markers for typing Aspergillus fumigatus isolates
International audienc
Diagnostic diffĂ©rentiel des espĂšces "Entamoeba histolytica" et Entamoeba dispar" dans les selles (Ă©valuation de la stratĂ©gie actuelle au GHU de la PitiĂ© SalpĂȘtriĂšre et mise au point d'une technique PCR en temps rĂ©el)
PARIS-BIUP (751062107) / SudocSudocFranceF
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