The effect of fat supplementation of concentrates on digestion and utilization of energy by productive dairy cows.

Two energy balance experiments each used 6 high-yielding dairy cows. In the first, a 3 X 3 Latin square with one replicate, rations were of 7 kg hay and 11 to 14 kg of concentrates having crude protein 251 g and gross energy 17.9 MJ/kg, given alone or with 5% tallow or 5% soya bean oil. Three weeks of adaptation were followed by 10 to 12 days when excreta were collected and gas exchange estimated. Digestibility and metabolizable energy of concentrates and hay were also assessed using wethers fed to maintenance only. Intakes of DM and digestibility of nutrients other than lipid did not differ among treatments with either sheep or cows. There were no apparent differences in rumen fermentation in the one cannulated cow used, and energy loss in urine was also unaffected. Lipid supplement reduced methane loss. Milk yield and milk energy were increased with tallow, with a simultaneous reduction of milk fat and percentage of protein. Soya bean oil reduced the percentage of milk fat and milk energy produced. The second trial, using similar animals and with similar management had a change-over design in which the concentrate was given alone or with 7% tallow. Results confirmed those of trial 1. The effect of level of feeding on the efficiency of utilization of energy is discussed. (Abstract retrieved from CAB Abstracts by CABI’s permission

Development and application of a SYBR Green RT-PCR for first line screening and quantification of porcine sapovirus infection

BACKGROUND: Sapoviruses are single stranded positive sense RNA viruses belonging to the family Caliciviridae. The virus is detected in different species including the human and the porcine species as an enteric pathogen causing asymptomatic to symptomatic enteritis. In this study, we report the development of a rapid real time qRT-PCR based on SYBR Green chemistry for the diagnosis of porcine sapovirus infection in swine. RESULTS: The method allows the detection of porcine sapoviruses and the quantification of the genomic copies present in stool samples. During its development, the diagnostic tool showed good correlation compared with the gold standard conventional RT-PCR and was ten-fold more sensitive. When the method was applied to field samples, porcine noroviruses from genogroup 2 genotype 11b were also detected. The method was also applied to swine samples from the Netherlands that were positive for PoSaV infection. Phylogenetic results obtained from the samples showed that PoSaV sequences were genetically related to the currently described genogroup III, to the proposed genogroup VII and also to the MI-QW19 sequence (close to the human SaV sequences). CONCLUSIONS: A rapid, sensitive, and reliable diagnosis method was developed for porcine sapovirus diagnosis. It correlated with the gold standard conventional RT-PCR. Specificity was good apart for genogroup 2 genotype 11b porcine noroviruses. As a first line screening diagnosis method, it allows a quicker and easier decision on doubtful samples

Balanced Scorecard voor inkoop

Een Balanced Scorecard kan ontwikkeld worden voor de hele organisatie, maar ook voor onderdelen daarvan. In dit artikel wordt ingegaan op de ontwikkeling van een Balanced Scorecard voor de inkoopafdelin