Discovery of extended radio emission in the young cluster Wd1

We present 10 micron, ISO-SWS and Australia Telescope Compact Array observations of the region in the cluster Wd1 in Ara centred on the B[e] star Ara C. An ISO-SWS spectrum reveals emission from highly ionised species in the vicinity of the star, suggesting a secondary source of excitation in the region. We find strong radio emission at both 3.5cm and 6.3cm, with a total spatial extent of over 20 arcsec. The emission is found to be concentrated in two discrete structures, separated by 14''. The westerly source is resolved, with a spectral index indicative of thermal emission. The easterly source is clearly extended and nonthermal (synchrotron) in nature. Positionally, the B[e] star is found to coincide with the more compact radio source, while the southerly lobe of the extended source is coincident with Ara A, an M2 I star. Observation of the region at 10micron reveals strong emission with an almost identical spatial distribution to the radio emission. Ara C is found to have an extreme radio luminosity in comparison to prior radio observations of hot stars such as O and B supergiants and Wolf-Rayet stars, given the estimated distance to the cluster. An origin in a detatched shell of material around the central star is therefore suggested; however given the spatial extent of the emission, such a shell must be relatively young (10^3 yrs). The extended non thermal emission associated with the M star Ara A is unexpected; to the best of our knowledge this is a unique phenomenon. SAX (2-10keV) observations show no evidence of X-ray emission, which might be expected if a compact companion were present.Comment: 5 pages including encapsulated figures, figure 3 separate. Accepted for MNRAS pink page

Methods to create a stringent selection system for mammalian cell lines

The efficient establishment of high protein producing recombinant mammalian cell lines is facilitated by the use of a stringent selection system. Here, we describe two methods to create a stringent selection system based on the Zeocin resistance marker. First, we cloned increasingly longer stretches of DNA, encoding a range of 8–131 amino acids immediately upstream of the Zeocin selection marker gene. The DNA stretches were separated from the open reading frame of the selection marker gene by a stopcodon. The idea behind this was that the translation machinery will first translate the small peptide, stop and then restart at the AUG of the Zeocin marker. This process, however, will become less efficient with increasingly longer stretches of DNA upstream of the Zeocin marker that has to be translated first. This would result in lower levels of the Zeocin selection marker protein and thus a higher selection stringency of the system. Secondly, we performed a genetic screen to identify PCR induced mutations in the Zeocin selection protein that functionally impair the selection marker protein. Both the insertion of increasingly longer peptides and several Zeocin selection protein mutants resulted in a decreasing number of stably transfected colonies that concomitantly displayed higher protein expression levels. When the Zeocin mutants were combined with very short small peptides (8–14 amino acids long), this created a flexible, high stringency selection system. The system allows the rapid establishment of few, but high protein producing mammalian cell lines

Not your average biker; Criminal careers of members of Dutch outlaw motorcycle gangs

Based on criminal career data of a sample of 601 police-identified outlaw motorcycle gang members and an age-matched comparison group of 300 non-gang affiliated motorcycle owners, the current analysis examines various dimensions of the criminal careers of outlaw bikers, including participation, onset, frequency, and crime mix. Results show that Dutch outlaw bikers are more often convicted than the average Dutch motorcyclist, and that these convictions not only pertain to minor offenses but also to serious and violent crimes. We find that outlaw bikers’ criminal careers differ from that of the average Dutch motorcyclist already during the juvenile and early adult years, but also – and more so – during the adult years. These results fit the enhancement hypothesis of gang membership and suggest that both selection of crime prone individuals in outlaw motorcycle gangs and facilitation of criminal behavior whilst in the gang are taking place.Criminal Justice: Legitimacy, accountability, and effectivit

The impact of first-time imprisonment on marriage and divorce:a matched samples comparison in a Dutch conviction cohort

Marriage has a prominent place in criminological theory and research as one institution that has the potential to genuinely foster desistance from a criminal career. Mass imprisonment policies in the United States and elsewhere, therefore, pose a potential threat of increased crime if they impede the ability of ex-prisoners to reintegrate into society by stigmatizing them and limiting their chances in the marriage market. We use a long-term study of a conviction cohort in The Netherlands to ascertain the effect that first-time imprisonment has on the likelihood of marriage and divorce. The results suggest that the effect of imprisonment on the likelihood of marriage (among unmarried offenders) is largely a selection artifact, although there is very weak evidence for a short-lived impact that does not persist past the first year post-release. This is interpreted as a residual incapacitation effect. On the other hand, the results strongly suggest that the experience of incarceration leads to a substantially higher divorce risk among offenders who are married when they enter prison

Measurements of the Casimir-Lifshitz force in fluids: the effect of electrostatic forces and Debye screening

In this work, we present detailed measurements of the Casimir-Lifshitz force between two gold surfaces (a sphere and a plate) immersed in ethanol and study the effect of residual electrostatic forces, which are dominated by static fields within the apparatus and can be reduced with proper shielding. Electrostatic forces are further reduced by Debye screening through the addition of salt ions to the liquid. Additionally, the salt leads to a reduction of the Casimir-Lifshitz force by screening the zero-frequency contribution to the force; however, the effect is small between gold surfaces at the measured separations and within experimental error. An improved calibration procedure is described and compared to previous methods. Finally, the experimental results are compared to Lifshitz's theory and found to be consistent for the materials used in the experiment.Comment: 11 figures. PRA in pres

Human Papillomavirus and Oropharyngeal Squamous Cell Carcinoma: A Case-Control Study regarding Tobacco and Alcohol Consumption

We aimed to determine the role of HPV in the pathogenesis and outcome of oropharyngeal squamous cell carcinoma (OSCC) in lifelong nonsmoking and nondrinking patients. A case-case analysis was performed to compare the presence of HPV-DNA in tumor cells of 16 nonsmoking and nondrinking with 16 matched smoking and drinking patients (matching criteria: age at incidence, gender, tumor sublocation, tumor stage). HPV was detected using 2 PCR tests, FISH analysis, and p16INK4A immunostaining. Nonsmoking and nondrinking patients had more HPV-positive tumors than smoking and drinking patients (n = 12; 75% versus n = 2; 13%; P < 0.001). All HPV-positive tumors showed p16INK4A overexpression, and 1 HPV-negative tumor had p16INK4A overexpression, (P < 0.001). Overall survival and disease-specific survival were higher for HPV-positive compared to HPV-negative cases (P = 0.027, P = 0.039, resp.). In conclusion, HPV is strongly associated with OSCC of nonsmoking and nondrinking patients. Specific diagnostic and therapeutic actions should be considered for these patients to achieve a better prognosis

Epidermal growth factor receptor expression in pancreatic lesions induced in the rat by azaserine.

In the present study, the expression of the epidermal growth factor receptor (EGFR) was investigated in putative preneoplastic and neoplastic acinar cell lesions induced in the rat pancreas by azaserine, using Northern blotting, in situ hybridisation (ISH) and immunohistochemistry. EGFR protein levels were decreased in putative preneoplastic eosinophilic acinar cell lesions (atypical acinar cell nodules, AACN) in comparison with normal acinar cells of the pancreas. However, EGFR mRNA expression correlated positively with the volume of AACN in pancreatic homogenates and ISH showed equal or stronger EGFR mRNA expression in AACN than in the surrounding normal acinar cells. Neither EGFR protein nor EGFR mRNA was detected in more advanced lesions such as acinar adenocarcinomas (in situ). Moreover, EGFR protein expression showed an inverse relationship with the mitotic rate of the acinar cells. These findings suggest that down-regulation of EGFR at the protein level may abrogate negative constraints on cell growth, which may stimulate the development of putative preneoplastic AACN to more advanced lesions and, ultimately, acinar adenocarcinomas