EXTRACELLULAR AND INTRACELLULAR SYNTHESIS OF SILVER NANOPARTICLES

ABSTRACTObjective: The cellular synthesis of nanoparticle is a green process and alternative for a conventional process for the preparation of silver nanoparticles.In our research, focus has been given to the development of an efficient and eco-friendly viable process for the synthesis of silver nanoparticles usingcancer and non-cancerous cells, a cell culture that was isolated. The results of this investigation are observed that silver nanoparticles could beinduced to synthesis intra- and extra-cellularly using mammalian cells such as cancerous and non-cancerous cells.Methods: The silver nanoparticles are synthesized by the cancer and non-cancerous cells such as HeLa (Homo sapiens, human), SiHa, and humanembryonic kidney-293 cell lines. The silver nanoparticles were characterized by ultraviolet (UV)-visible spectroscopy, transmission electronmicroscopy (TEM), and X-ray powder diffraction (XRD).Results: The silver nanoparticles exhibited maximum absorbance at 415 nm in UV-visible spectroscopy. The XRD confirms the characteristic of thecrystal lattice of silver nanoparticles by observing three peaks: Peak at 38 is due to reflection from (111), peak at 44 is due to reflection from (200),and peak at 65 is due to reflection from (220). TEM images showed the formation of stable silver nanoparticles in the cell lines.Conclusion: The method of extraction of intracellular/extracellular synthesis of silver nanoparticles was inexpensive, simple, and effective in largescale with no need to use of complex process equipment. The cancer cell considered as a biological factory at nanoscale dimension which continued togrow after synthesis of silver nanoparticles. The silver reduction by these cancer cells has occurred through energy-dependent processes that lead tothe high output of this reaction. Hence, this new approach of using a mammalian cell for the successful synthesis of nanosized silvers could be easilyscaled up, which establishes its commercial viability and also useful in the drug delivery and drug targeting.Keywords: Silver nanoparticles, Cancer cells, Biosynthesis and characteristics of silver nanoparticles.Â

Algebraic Properties of Parikh Matrices of Words under an Extension of Thue Morphism

The Parikh matrix of a word $w$ over an alphabet $\{a_1, \cdots , a_k \}$ with an ordering $a_1 < a_2 < \cdots a_k,$ gives the number of occurrences of each factor of the word $a_1 \cdots a_k$ as a (scattered) subword of the word $w.$ Two words $u,v$ are said to be $M-$equivalent, if the Parikh matrices of $u$ and $v$ are the same. On the other hand properties of image words under different morphisms have been studied in the context of subwords and Parikh matrices. Here an extension to three letters, introduced by S$\acute{e}\acute{e}$bold (2003), of the well-known Thue morphism on two letters, is considered and properties of Parikh matrices of morphic images of words are investigated. The significance of the contribution is that various classes of binary words are obtained whose images are $M-$equivalent under this extended morphism

Computing with Membranes and Picture Arrays

Splicing systems were introduced by Tom Head [3] on biological considerations to model certain recombinant behaviour of DNA molecules. An effective extension of this operation to images was introduced by Helen Chandra et al. [5] and H array splicing systems were considered. A new method of applying the splicing operation on images of hexagonal arrays was introduced by Thomas et al. [12] and generated a new class of hexagonal array languages HASSL. On the other hand, P systems, introduced by Paun [6] generating rectangular arrays and hexagonal arrays have been studied in the literature, bringing together the two areas of theoretical computer science namely membrane computing and picture languages. P system with array objects and parallel splicing operation on arrays is introduced as a simple and effective extension of P system with operation of splicing on strings and this new class of array languages is compared with the existing families of array languages. Also we propose another P system with hexagonal array objects and parallel splicing operation on hexagonal arrays is introduced and this new class of hexagonal array languages is compared with the existing families of hexagonal array languages

EpCAM aptamer mediated cancer cell specific delivery of EpCAM siRNA using polymeric nanocomplex

BACKGROUND: Epithelial cell adhesion molecule (EpCAM) is overexpressed in solid tumors and regarded as a putative cancer stem cell marker. Here, we report that employing EpCAM aptamer (EpApt) and EpCAM siRNA (SiEp) dual approach, for the targeted delivery of siRNA to EpCAM positive cancer cells, efficiently inhibits cancer cell proliferation. RESULTS: Targeted delivery of siRNA using polyethyleneimine is one of the efficient methods for gene delivery, and thus, we developed a novel aptamer-PEI-siRNA nanocomplex for EpCAM targeting. PEI nanocomplex synthesized with EpCAM aptamer (EpApt) and EpCAM siRNA (SiEp) showed 198&nbsp;nm diameter sized particles by dynamic light scattering, spherical shaped particles, of 151&thinsp;&plusmn;&thinsp;11&nbsp;nm size by TEM. The surface charge of the nanoparticles was -30.0&nbsp;mV using zeta potential measurements. Gel retardation assay confirmed the PEI-EpApt-SiEp nanoparticles formation. The difference in size observed by DLS and TEM could be due to coating of aptamer and siRNA on PEI nanocore. Flow cytometry analysis revealed that PEI-EpApt-SiEp has superior binding to cancer cells compared to EpApt or scramble aptamer (ScrApt) or PEI-ScrApt-SiEp. PEI-EpApt-SiEp downregulated EpCAM and inhibited selectively the cell proliferation of MCF-7 and WERI-Rb1 cells. CONCLUSIONS: The PEI nanocomplex fabricated with EpApt and siEp was able to target EpCAM tumor cells, deliver the siRNA and silence the target gene. This nanocomplex exhibited decreased cell proliferation than the scrambled aptamer loaded nanocomplex in the EpCAM expressing cancer cells and may have potential for EpCAM targeting in vivo

A Comprehensive Survey of Deep Learning: Advancements, Applications, and Challenges

Artificial intelligence's "deep learning" discipline has taken off, revolutionizing a variety of industries, from computer vision and natural language processing to healthcare and finance. Deep learning has shown extraordinary effectiveness in resolving complicated issues, and it has a wide range of potential applications, from autonomous vehicles to healthcare. The purpose of the survey to study deep learning's present condition, including recent advancements, difficulties, and constraints since the subject is currently fast growing. The basic ideas of deep learning, such as neural networks, activation functions, and optimization algorithms, are first introduced. We next explore numerous topologies, emphasizing their distinct properties and uses, including convolutional neural networks (CNNs), recurrent neural networks (RNNs), and generative adversarial networks (GANs). Further concepts, applications, and difficulties of deep learning are all covered in this survey paper's thorough review. This survey aid the academics, professionals, and individuals who want to learn more about deep learning and explore its applications to challenging situations in the real world

Synthesis and crystal structures of 5'-phenylspiro[indoline-3, 2'-pyrrolidin]-2-one derivatives

<p>Abstract</p> <p>Background</p> <p>The spiro- indole-pyrrolidine ring system is a frequently encountered structural motif in many biologically important and pharmacologically relevant alkaloids. The derivatives of spirooxindole ring systems are used as antimicrobial, antitumour agents and as inhibitors of the human NKI receptor besides being found in a number of alkaloids like horsifiline, spirotryprostatin and (+) elacomine. The recently discovered small-molecule MDM2 inhibitor MI-219 and its analogues are in advanced preclinical development as cancer therapeutics.</p> <p>Results</p> <p>In the crystal structures of the two organic compounds, 4'-Nitro-3',5'-diphenylspiro[indoline-3,2'-pyrrolidin]-2-one and 3'-(4-Methoxyphenyl)- 4'-nitro -5'-phenylspiro[indoline-3,2'-pyrrolidin]-2-one, N-H···O hydrogen bonds make the R²₂(8) ring motif. Further, the structures are stabilized by intermolecular hydrogen bonds.</p> <p>Conclusion</p> <p>The crystal structures of 4'-Nitro-3',5'-diphenylspiro[indoline-3,2'-pyrrolidin]-2-one and 3'-(4-Methoxyphenyl)- 4'-nitro -5'-phenylspiro[indoline-3,2'-pyrrolidin]-2-one have been investigated in detail. In both the compounds, the R²₂(8) motif is present. Due to the substitution of methoxyphenyl instead of phenyl ring, the entire configuration is inverted with respect to the 2-oxyindole ring.</p