Cryo-EM structure of the potassium-chloride cotransporter KCC4 in lipid nanodiscs.

Cation-chloride-cotransporters (CCCs) catalyze transport of Cl- with K+ and/or Na+across cellular membranes. CCCs play roles in cellular volume regulation, neural development and function, audition, regulation of blood pressure, and renal function. CCCs are targets of clinically important drugs including loop diuretics and their disruption has been implicated in pathophysiology including epilepsy, hearing loss, and the genetic disorders Andermann, Gitelman, and Bartter syndromes. Here we present the structure of a CCC, the Mus musculus K+-Cl- cotransporter (KCC) KCC4, in lipid nanodiscs determined by cryo-EM. The structure, captured in an inside-open conformation, reveals the architecture of KCCs including an extracellular domain poised to regulate transport activity through an outer gate. We identify binding sites for substrate K+ and Cl- ions, demonstrate the importance of key coordinating residues for transporter activity, and provide a structural explanation for varied substrate specificity and ion transport ratio among CCCs. These results provide mechanistic insight into the function and regulation of a physiologically important transporter family

Emotional Performance and Antiracism in the Writing Center

Why do conversations regarding students’ right to their own language and antiracism in the writing center still invite insults and agitation? After all, these struggles for students’ rights to selfdetermination and their own language in composition are far from new. The narratives present within this writing move beyond mere analysis of how and why established institutions attempt to control, and, rather, put Laura Micciche’s theories of emotion and performance to the test. When teaching tutor training, readings regarding students' right to their own language and race potentially cause conflict and can, at least at first, elicit strong emotional responses. This article explores the value of such early emotional reactions to these readings. Can the tutors’ emotional performances, both in action and voice, eventually help to bring attention to, or subvert the backlash and attacks antiracism rhetoric tends to invite? Within its pages, Micciche’s Doing Emotion: Rhetoric, Writing, Teaching suggests that we perform emotional appeals rather than simply make them. Through performance, she claims, we present emotion, not as something that resides in people to be shared or withheld, but as encounters between people. This article’s narrative “reenactments,” then, are set to reveal the fears and desires behind the resistance I’ve both witnessed and encountered all while promoting what I deem to be a necessity for emotional performance in antiracism and writing center work.University Writing Cente

Numerical and semi-analytic core mass distributions in supersonic isothermal turbulence

We investigate the influence of the turbulence forcing on the mass distributions of gravitationally unstable cores by postprocessing data from simulations of non-selfgravitating isothermal supersonic turbulence with varying resolution. In one set of simulations solenoidal forcing is applied, while the second set uses purely compressive forcing to excite turbulent motions. From the resulting density field, we compute the mass distribution of gravitationally unstable cores by means of a clump-finding algorithm. Using the time-averaged probability density functions of the mass density, semi-analytic mass distributions are calculated from analytical theories. We apply stability criteria that are based on the Bonnor-Ebert mass resulting from the thermal pressure and from the sum of thermal and turbulent pressure. Although there are uncertainties in the application of the clump-finding algorithm, we find systematic differences in the mass distributions obtained from solenoidal and compressive forcing. Compressive forcing produces a shallower slope in the high-mass power-law regime compared to solenoidal forcing. The mass distributions also depend on the Jeans length resulting from the choice of the mass in the computational box, which is freely scalable for non-selfgravitating isothermal turbulence. Provided that all cores are numerically resolved and most cores are small compared to the length scale of the forcing, the normalised core mass distributions are found to be close to the semi-analytic models. Especially for the high-mass tails, the Hennebelle-Chabrier theory implies that the additional support due to turbulent pressure is important.Comment: 15 pages, 7 figures, submitted to A&

Complete sets of cyclic mutually unbiased bases in even prime power dimensions

We present a construction method for complete sets of cyclic mutually unbiased bases (MUBs) in Hilbert spaces of even prime power dimensions. In comparison to usual complete sets of MUBs, complete cyclic sets possess the additional property of being generated by a single unitary operator. The construction method is based on the idea of obtaining a partition of multi-qubit Pauli operators into maximal commuting sets of orthogonal operators with the help of a suitable element of the Clifford group. As a consequence, we explicitly obtain complete sets of cyclic MUBs generated by a single element of the Clifford group in dimensions $2^m$ for $m=1,2,...,24$.Comment: 10 page

Short-lived Radio Bursts from the Crab Pulsar

Our high-time-resolution observations reveal that individual main pulses from the Crab pulsar contain one or more short-lived microbursts. Both the energy and duration of bursts measured above 1 GHz can vary dramatically in less than a millisecond. These fluctuations are too rapid to be caused by propagation through turbulence in the Crab Nebula or the interstellar medium; they must be intrinsic to the radio emission process in the pulsar. The mean duration of a burst varies with frequency as $\nu^{-2}$, significantly different from the broadening caused by interstellar scattering. We compare the properties of the bursts to some simple models of microstructure in the radio emission region.Comment: 34 pages, 10 figures; accepted for publication in Ap

Emulating Simulations of Cosmic Dawn for 21cm Power Spectrum Constraints on Cosmology, Reionization, and X-ray Heating

Current and upcoming radio interferometric experiments are aiming to make a statistical characterization of the high-redshift 21cm fluctuation signal spanning the hydrogen reionization and X-ray heating epochs of the universe. However, connecting 21cm statistics to underlying physical parameters is complicated by the theoretical challenge of modeling the relevant physics at computational speeds quick enough to enable exploration of the high dimensional and weakly constrained parameter space. In this work, we use machine learning algorithms to build a fast emulator that mimics expensive simulations of the 21cm signal across a wide parameter space to high precision. We embed our emulator within a Markov-Chain Monte Carlo framework, enabling it to explore the posterior distribution over a large number of model parameters, including those that govern the Epoch of Reionization, the Epoch of X-ray Heating, and cosmology. As a worked example, we use our emulator to present an updated parameter constraint forecast for the Hydrogen Epoch of Reionization Array experiment, showing that its characterization of a fiducial 21cm power spectrum will considerably narrow the allowed parameter space of reionization and heating parameters, and could help strengthen Planck's constraints on $\sigma_8$. We provide both our generalized emulator code and its implementation specifically for 21cm parameter constraints as publicly available software.Comment: 22 pages, 9 figures; accepted to Ap

Controlling quantum systems by embedded dynamical decoupling schemes

A dynamical decoupling method is presented which is based on embedding a deterministic decoupling scheme into a stochastic one. This way it is possible to combine the advantages of both methods and to increase the suppression of undesired perturbations of quantum systems significantly even for long interaction times. As a first application the stabilization of a quantum memory is discussed which is perturbed by one-and two-qubit interactions

Microsurgical Technique of Simultaneous Pancreas/Kidney Transplantation in the Rat: Clinical Experience and Review of the Literature

Background: For experimental basic research, standardized transplantation models reflecting technical and immunologic aspects are necessary. This article describes an experimental model of combined pancreas/kidney transplantation (PKTx) in detail. Materials and Methods: Donor rats underwent en bloc pancreatectomy and nephrectomy. Revascularization was performed using the aorta with the superior mesenteric artery and the inferior vena cava with the portal vein. Exocrine drainage of the pancreas took place over a segment of the duodenum which was transplanted side-to-side to the jejunum. The kidney vessels were transplanted end-to-side. The ureter was anastomosed by patch technique. Postoperatively, serum parameters were monitored daily. Biopsies for histopathology were taken on days 5, 8 and 12. Results: All 12 recipients survived the combined PKTx without serious surgical complications. One thrombosis of the portal vein led to organ failure. Blood glucose levels were normal by the 3rd postoperative day. The transplanted duodenal segment showed slight villous atrophy, and the kidneys were well perfused without vascular complications. The anastomosis between ureter and bladder was leakproof. Conclusions: Excellent graft function and survival rates can be achieved due to simplified operation technique and short operation time. It may thus have high clinical relevance to immunologic issues within the scope of basic research. Copyright (C) 2009 S. Karger AG, Base