The effect of granulocyte colony stimulating factor on genotoxicity in allogeneic peripheral blood stem cell transplantation donors: A prospective case-control study

Background. Every year, thousands of donors are exposed to granulocyte-colony stimulating factor (G-CSF) for stem cell mobilization in hematopoietic stem cell transplantations (HSCT). Previous studies about the genotoxicity of G-CSF were inconclusive. In this study, the genotoxic effects of G-CSF in peripheral blood stem cell (PBSC) donors were evaluated prospectively by using three different validated and reliable methods for the first time in the literature to the best of our knowledge. Methods. Donors of PBSC transplantation (n=36), who received G-CSF were evaluated for genotoxicity by micronucleus test (MNT), nuclear division index (NDI), and comet assay (CA). Genotoxic effects are expected to cause an increase in MNT and CA values and decrease in NDI. Blood samples were collected at three time-points (TP): before starting G-CSF (TP1), after G-CSF for five days (TP2), and one month after the last dose (TP3). Sixteen controls were included for baseline comparison of genotoxicity tests. CD34 cell counts and hemograms were also analyzed. Results. MNT and CA parameters; comet and tail length, tail DNA%, and tail moment, showed no change in time whereas another CA parameter, Olive’s tail moment (OTM) was increased significantly at TP3 compared to both baseline and TP2 (p=0.002 and p=0.017, respectively). Nuclear division index decreased significantly at TP2 (p[removed]Ankara University Scientific Study Fun

Validation and Optimization of an Analytical Method Based on Cold Vapor Atomic Absorption Spectrometry for the Determination of Mercury in Maternal Blood, Cord Blood, and Placenta Samples

Yuksel, Bayram/0000-0001-7686-8648; Kaya-Akyuzlu, Dilek/0000-0002-3305-0587WOS: 000410870100006In this study, a simple and sensitive cold vapor atomic absorption spectrometry (CVAAS) method was developed and validated for the determination of mercury in maternal blood, cord blood, and placenta samples. Biological samples were obtained from 113 mother -newborn pairs. Infant characteristics such as birth weight, birth length, and head circumference were recorded. Microwave-assisted acid digestion was applied and a cold vapor module was utilized for atomization. The method showed linearity in the range of 0-20 pg/L with a detection and quantification limit equal to 0.231.1g/L and 0.76 pg/L, respectively, as well as very good repeatability not exceeding 3%. The calibration curve was characterized by a high correlation coefficient (r2=0.9992). Validation was performed in terms of precision and accuracy with the use of certified reference materials (CRM). The method was applied to the analysis of spiked CRM samples yielding satisfactory results (97.4-101.2%). The mean mercury levels of maternal blood, placentas, and cord blood were 3.23 1.46 pg/L, 12.24 11.50 g/kg, 3.69 3.44 pg/L, respectively. As a result of the statistical analysis, a significant correlation was found between placenta-Hg and cord blood-Hg levels (r=+0.231, p=0.014). In addition, birth head circumference and cord blood -Hg levels were found to be correlated to each other (r=+0.318, p=0.033).Ankara University Scientific Research Projects Coordination Unit (BAP)Ankara University [15B0217001]This study was financially supported by the Ankara University Scientific Research Projects Coordination Unit (BAP). Project Number: 15B0217001