85 research outputs found
Nematode control in suckler beef cattle over their first two grazing seasons using a targeted selective treatment approach
peer-reviewedBackground
With concerns over the development of anthelmintic resistance in cattle nematode populations, we must re-examine our approach to nematode control in cattle. Targeted selective treatments (TST), whereby individual animals are treated instead of entire groups, are being investigated as an alternative. The study objective was to determine if anthelmintic usage could be reduced using a TST-based approach to nematode control in spring-born suckler beef cattle over their first and second grazing seasons (SGS) without affecting performance. In the first grazing season (FGS), 99 calves with an initial mean (s.d.) calf age and live weight on day 0 (June 28th 2012) of 107 (23.1) days and 160 (32.5) kg, respectively, were used. The study commenced on day 0 when calves were randomised and allocated to one of two treatments; 1), standard treatment (control) and 2), TST. Control calves were treated subcutaneously with ivermectin on days 0, 41 and 82 in the FGS. All calves were treated with ivermectin on day 124 and housed on day 133. In the SGS, only heifer calves from the FGS were used and control heifers were treated with ivermectin on day 393. Animals were weighed, blood and faecal sampled every three weeks. The TST animals were treated with ivermectin if thresholds based on a combination of plasma pepsinogen concentrations, faecal egg count and/or the presence of Dictyocaulus viviparus larvae in faeces (FGS only) were reached.
Results
No TST calves reached the treatment threshold criteria in the FGS. The FGS average daily live weight gain (ADG ± s.e.m.) for control and TST group calves was 0.89 ± 0.02 kg and 0.94 ± 0.02 kg day−1, respectively (P = 0.17). In the SGS, all heifers were treated with ivermectin on day 431 due to clinical signs of respiratory disease. The ADG for control and TST heifers from turnout on day 321 to day 431 was 0.90 ± 0.04 and 0.80 ± 0.04 kg day−1, respectively (P = 0.03).
Conclusions
Spring-born FGS suckler beef calves require minimal anthelmintic treatment to maintain performance. In contrast, clinical parasitic disease may develop in the SGS unless appropriate anthelmintic treatment is provided
Anthelmintic resistance among gastrointestinal nematodes of cattle on dairy calf to beef farms in Ireland
peer-reviewedBackground
The control of gastrointestinal nematodes (GIN) of cattle in pasture-based production systems such as Ireland is highly dependent on the availability of efficacious anthelmintics. There is very little information available on the efficacy of the broad-spectrum anthelmintics against GIN of cattle in Ireland and the aim of this study was to determine the prevalence of anthelmintic resistance on dairy calf to beef farms.
Results
GIN burden was monitored on thirty-six recruited farms by performing herd level faecal egg counts (FEC) every 2 weeks. Of these, nine farms were lost from the study as calves were treated with an anthelmintic for Dictyocaulus viviparus, two were lost as they treated for GIN, one dropped out of the study and on one the herd FEC did not reach the threshold for carrying out the Faecal Egg Count Reduction Test (FECRT). On the remaining 23 farms, once the herd FEC reached 100 eggs per gram, a FECRT was carried out. Pre and post-treatment larval cultures were also performed to identify the GIN to genus level. The efficacy of fenbendazole, levamisole, ivermectin and moxidectin was evaluated on 15, 11, 16 and 11 farms respectively. Resistance to fenbendazole was identified on 9 farms (60%) with resistance suspected on a further farm. Resistance to levamisole, ivermectin and moxidectin was detected on 2 (18%), 16 (100%) and 8 (73%) farms respectively. The predominant genera detected pre and post-treatment were Cooperia and Ostertagia with both genera detected post-treatment with fenbendazole and ivermectin. Due to the low proportion of Ostertagia spp. pre-treatment, the efficacy of levamisole or moxidectin against this genus could not be reliably established.
Conclusions
Anthelmintic resistance was widespread on the sampled dairy calf to beef farms in Ireland with resistance to benzimidazole, levamisole, ivermectin and moxidectin detected
Gastrointestinal nematode control practices on lowland sheep farms in Ireland with reference to selection for anthelmintic resistance
Gastrointestinal parasitism is a widely recognised problem in sheep production, particularly for lambs. While anthelmintics have a pivotal role in controlling the effects of parasites, there is a paucity of data on how farmers use anthelmintics. A representative sample of Irish lowland farmers were surveyed regarding their parasite control practices and risk factors that may contribute to the development of anthelmintic resistance. Questionnaires were distributed to 166 lowland Irish sheep producers. The vast majority of respondents treated their sheep with anthelmintics. Lambs were the cohort treated most frequently, the majority of farmers followed a set programme as opposed to treating at sign of disease. A substantial proportion (61%) administered four or more treatments to lambs in a 'normal' year. Departures from best practice in anthelmintic administration that would encourage the development of anthelmintic resistance were observed. In conclusion, in the light of anthelmintic resistance, there is a need for a greater awareness of the principles that underpin the sustainable use of anthelmintics and practices that preserve anthelmintic efficacy should be given a very high priority in the design of helminth control programmes on each farm. To this end, given that veterinary practitioners and agricultural advisors were considered to be the farmer's most popular information resource, the capacity of these professions to communicate information relating to best practice in parasite control should be targeted
High level of treatment failure with commonly used anthelmintics on Irish sheep farms
peer-reviewedBackground: In 2013 a Technology Adoption Program for sheep farmers was established to encourage the implementation of best management practices on sheep farms in Ireland. There were 4,500 participants in this programme in 2013. As part of this programme, farmers had the option to carry out a drench test to establish the efficacy of their anthelmintic treatment.
Results: Flock faecal samples were collected before and after treatment administration and gastrointestinal nematode eggs enumerated. In total there were 1,893 participants in the task, however only 1,585 included both a pre- and post-treatment faecal sample. Of those, 1,308 provided information on the anthelmintic product that they used with 46%, 23% and 28% using a benzimidazole (BZ), levamisole (LEV) and macrocyclic lactone (ML) product respectively. The remaining farmers used a product inapplicable for inclusion in the task such as a flukicide or BZ/LEV combination product. Samples were included for analysis of drench efficacy if the pre-treatment flock egg count was ≥200 eggs per gram and the interval post-sampling was 10–14 days for BZ products, 4–7 days for LEV products and 14–18 days for ML products. These criteria reduced the number of valid tests to 369, 19.5% of all tests conducted. If the reduction post-treatment was ≥95% the treatment was considered effective. Only 51% of treatments were considered effective using this criterion. There was a significant difference in efficacy between the anthelmintic drug classes with BZ effective in only 30% of treatments, LEV effective in 52% of cases and ML effective in 76% of cases.
Conclusions: Gastrointestinal nematode anthelmintic treatments, as practiced on Irish farms, have a high failure rate. There was a significant difference between the efficacies of the anthelmintic classes with BZ the least effective and ML the most effective
Growth hormone treatment in children with short stature born small for gestational age: 5-year results of a randomized, double-blind, dose-response trial
The growth-promoting effect of continuous GH treatment was evaluated over
5 yr in 79 children with short stature (height SD score, less than -1.88)
born small for gestational age (SGA; birth length SD score, less than
-1.88). Patients were randomly and blindly assigned to 1 of 2 GH dosage
groups (3 vs. 6 IU/m2 body surface-day). GH deficiency was not an
exclusion criterium. After 5 yr of GH treatment almost every child had
reached a height well within the normal range for healthy Dutch children
and in the range of their target height SD score. Only in children who
remained prepubertal during the study period was the 5-yr increase in
height SD score (HSDS) for chronological age significantly higher in the
study group receiving 6 compared to 3 IU GH/m2 x day. Remarkably, the 5-yr
increment in HSDS for chronological age was not related to spontaneous GH
secretion, maximum GH levels after provocation, or baseline insulin-like
growth factor I levels. GH treatment was associated with an acceleration
of bone maturation regardless of the GH dose given. The HSDS for bone age
and predicted adult height increased significantly. GH treatment was well
tolerated. In conclusion, our 5-yr data show that long term continuous GH
treatment at a dose of 3 or 6 IU/m2 x day in short children born SGA
results in a normalization of height during childhood followed by growth
along the target height percentile
Nurses’ worry or concern and early recognition of deteriorating patients on general wards in acute care hospitals: a systematic review
INTRODUCTION:Nurses often recognize deterioration in patients through intuition rather than through routine measurement of vital signs. Adding the 'worry or concern' sign to the Rapid Response System provides opportunities for nurses to act upon their intuitive feelings. Identifying what triggers nurses to be worried or concerned might help to put intuition into words, and potentially empower nurses to act upon their intuitive feelings and obtain medical assistance in an early stage of deterioration. The aim of this systematic review is to identify the signs and symptoms that trigger nurses' worry or concern about a patient's condition.METHODS:We searched the databases PubMed, CINAHL, Psychinfo and Cochrane Library (Clinical Trials) using synonyms related to the three concepts: 'nurses', 'worry/concern' and 'deterioration'. We included studies concerning adult patients on general wards in acute care hospitals. The search was performed from the start of the databases until 14 February 2014.RESULTS:The search resulted in 4,006 records, and 18 studies (five quantitative, nine qualitative and four mixed-methods designs) were included in the review. A total of 37 signs and symptoms reflecting the nature of the criterion worry or concern emerged from the data and were summarized in 10 general indicators. The results showed that worry or concern can be present with or without change in vital signs.CONCLUSIONS:The signs and symptoms we found in the literature reflect the nature of nurses' worry or concern, and nurses may incorporate these signs in their assessment of the patient and their decision to call for assistance. The fact that it is present before changes in vital signs suggests potential for improving care in an early stage of deterioration
Development of a multiplex fluorescence immunological assay for the simultaneous detection of antibodies against Cooperia oncophora, Dictyocaulus viviparus and Fasciola hepatica in cattle
Background A major constraint for the effective control and management of
helminth parasites is the lack of rapid, high-throughput, routine diagnostic
tests to assess the health status of individual animals and herds and to
identify the parasite species responsible for these helminthoses. The
capability of a multiplex platform for the simultaneous detection of three
pasture associated parasite species was evaluated and compared to existing
ELISAs. Methods The recombinant antigens 14.2 kDa ES protein for Cooperia
oncophora, major sperm protein for Dictyocaulus viviparus and Cathepsin L1 for
Fasciola hepatica were recombinantly expressed either in Escherichia coli or
Pichia pastoris. Antigens were covalently coupled onto magnetic beads. Optimal
concentrations for coupling were determined following the examination of serum
samples collected from experimentally mono-infected animals, before and after
their infection with the target species. Absence of cross-reactivity was
further determined with sera from calves mono-infected with Haemonchus
contortus, Ostertagia ostertagi and Trichostrongylus colubriformis.
Examination of negative serum samples was characterised by low median
fluorescence intensity (MFI). Results Establishment of the optimal serum
dilution of 1:200 was achieved for all three bead sets. Receiver Operating
Characteristic analyses were performed to obtain cut-off MFI values for each
parasite separately. Sensitivity and specificity at the chosen cut-off values
were close to, or 100 % for all bead sets. Examination of serum samples
collected on different days post infection from different animals showed a
high reproducibility of the assays. Serum samples were additionally examined
with two already established ELISAs, an in-house ELISA using the recombinant
MSP as an antigen and a DRG ELISA using Cathepsin L1 for liver fluke. The
results between the assays were compared and kappa tests revealed an overall
good agreement. Conclusions A versatile bead-based assay using fluorescence
detection (xMAP® technology) was developed to simultaneously detect antibodies
against C. oncophora, D. viviparus and F. hepatica in cattle serum samples.
This platform provides rapid, high-throughput results and is highly sensitive
and specific in comparison to existing serological as well as coproscopical
diagnostic techniques
Identification and epidemiological analysis of Perostrongylus falciformis infestation in Irish badgers
Publication history: Accepted - 24 June 2019; Published Online - 9 July 2019Background: The lungworm, Perostrongylus falciformis (fomerly known as Aelurostrongylus falciformis) has been
identified in badgers (Meles meles) in Britain, the Russian Federation, Italy, Norway, Poland, Ukraine, Bosnia Herzegovina
and Romania, while Aelurostrongylus pridhami has been reported from badgers in Spain.
Results: Pulmonary tissue from 1580 Irish badgers was examined and an estimated prevalence of 32.09% (95% CI:
29.79–34.45%) of this parasite was detected. Confirmation of its occurrence was made by PCR analysis on a subset of
the population.
Conclusion: Infestation was widely distributed throughout the Republic of Ireland, with a trend towards higher
infestation risk in western versus eastern counties. In addition males were at a higher risk of infestation than females
and juveniles were at a significantly higher risk than adult badgers
Lack of Middle East Respiratory Syndrome Coronavirus Transmission in Rabbits
Middle East respiratory syndrome coronavirus (MERS-CoV) transmission from dromedaries
to humans has resulted in major outbreaks in the Middle East. Although some other livestock animal
species have been shown to be susceptible to MERS-CoV, it is not fully understood why the spread of
the virus in these animal species has not been observed in the field. In this study, we used rabbits to
further characterize the transmission potential of MERS-CoV. In line with the presence of MERS-CoV
receptor in the rabbit nasal epithelium, high levels of viral RNA were shed from the nose following
virus inoculation. However, unlike MERS-CoV-infected dromedaries, these rabbits did not develop
clinical manifestations including nasal discharge and did shed only limited amounts of infectious
virus from the nose. Consistently, no transmission by contact or airborne routes was observed in
rabbits. Our data indicate that despite relatively high viral RNA levels produced, low levels of
infectious virus are excreted in the upper respiratory tract of rabbits as compared to dromedary
cam
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