Distância genética em caprinos naturalizados por meio de microssatélites de DNA.

Há uma necessidade de adotar estratégias de conservação para as populações naturalizadas de animais domésticos para assegurar a manutenção destas para o futuro. Este estudo visa contribuir para o conhecimento da variabilidade genética por meio de marcadores de microssatélites em caprinos naturalizados Moxotó, Canindé e Marota. Foram amostrados 124 animais adultos dos grupos Moxotó, Canindé e Marota dos núcleos de conservação da Embrapa. O DNA extraído foi amplificado mediante a reação em cadeia polimerase (PCR) e genotipado através do programa Fragment Profile (Amersham Bioscience). O método UPGMA (Unweighted Pair Group Method With Arithmetic Mean) foi utilizado para construção do dendograma com bootstrap (1000 repetições) pelo programa TFPGA (Miller, 1997). O dendograma agrupou no primeiro nódulo as populações Moxotó e Canindé e o bootstrap apresentou 75% de similaridade neste nódulo. No segundo nódulo houve a inclusão das três populações e obteve bootstrap de 100%. O número de loci suportando cada nódulo foram quatro e cinco, respectivamente. O método UPGMA, utilizando as distâncias de Nei (1978), permitiu o discernimento das populações caprinas com base em dados moleculares

Endometrial Cancer Spheres Show Cancer Stem Cells Phenotype and Preference for Oxidative Metabolism

This study aimed to characterize endometrial cancer regarding cancer stem cells (CSC) markers, regulatory and differentiation pathways, tumorigenicity and glucose metabolism. Endometrial cancer cell line ECC1 was submitted to sphere forming protocols. The first spheres generation (ES1) was cultured in adherent conditions (G1). This procedure was repeated and was obtained generations of spheres (ES1, ES2 and ES3) and spheres-derived cells in adherent conditions (G1, G2 and G3). Populations were characterized regarding CD133, CD24, CD44, aldehyde dehydrogenase (ALDH), hormonal receptors, HER2, P53 and β-catenin, fluorine-18 fluorodeoxyglucose ([18F]FDG) uptake and metabolism by NMR spectroscopy. An heterotopic model evaluated differential tumor growth. The spheres self-renewal was higher in ES3. The putative CSC markers CD133, CD44 and ALDH expression were higher in spheres. The expression of estrogen receptor (ER)α and P53 decreased in spheres, ERβ and progesterone receptor had no significant changes and β-catenin showed a tendency to increase. There was a higher 18F-FDG uptake in spheres, which also showed a lower lactate production and an oxidative cytosol status. The tumorigenesis in vivo showed an earlier growth of tumours derived from ES3. Endometrial spheres presented self-renewal and differentiation capacity, expressed CSC markers and an undifferentiated phenotype, showing preference for oxidative metabolism.info:eu-repo/semantics/publishedVersio

The Apollo ATCA Platform

We have developed a novel and generic open-source platform - Apollo - which simplifies the design of custom Advanced Telecommunications Computing Architecture (ATCA) blades by factoring the design into generic infrastructure and application-specific parts. The Apollo "Service Module" provides the required ATCA Intelligent Platform Management Controller, power entry and conditioning, a powerful system-on-module (SoM) computer, and flexible clock and communications infrastructure. The Apollo "Command Module" is customized for each application and typically includes two large field-programmable gate arrays, several hundred optical fiber interfaces operating at speeds up to 28 Gbps, memories, and other supporting infrastructure. The command and service module boards can be operated together or independently on the bench without need for an ATCA shelf.Comment: Submitted to the Proceedings for TWEPP 201

Programa de mejoramiento genetico de la raza girolando - Sumário de toros - Resultado de la prueba de progenie - Julio/2013.

bitstream/item/90486/1/DOC-165-Girolando-Espanhol-2013-completo.pd

[autosomal Chronic Granulomatous Disease: Case Report And Mutation Analysis Of Two Brazilian Siblings].

To report the case of two siblings with chronic granulomatous disease. Chronic granulomatous disease is a primary immunodeficiency disorder characterized by abnormal microbicidal activity. Mutations in the p47-phox gene (NCF-1) are present in about 30% of the patients with chronic granulomatous disease; this group presents a better prognosis and later onset of recurrent infections as compared with the X-linked variant, present in about 56% of patients. Case 1 is a female presenting repeat infections since age 10, starting with impetigo followed by severe pneumonia six months later. The severity of the lung infection associated with liver abscess and the patient's resistance to treatment prompted laboratory investigation for immunodeficiency. The results of the nitroblue tetrazolium and superoxide release tests were consistent with a diagnosis of chronic granulomatous disease. The parents and siblings were assessed, revealing the presence of granulomatous disease in a brother (Case 2). He also presented repeat infections with impetigo at age 10, followed by pneumonia six months later, however in a non severe form. Single-strand conformational polymorphism analysis detected abnormal electrophoretic mobility of exon 2 of the NCF-1 gene. Sequence DNA analysis revealed a dinucleotide GT deletion in exon 2. It is important to evaluate the relatives of chronic granulomatous disease patients, even in the absence of typical clinical signs. Defining the mutation and its correlation with phenotype is important to provide appropriate genetic counseling and clinical prognosis.80425-