Within- and between-pen transmission of Classical Swine Fever Virus: a new method to estimate the basic reproduction ratio from transmission experiments

We present a method to estimate basic reproduction ratio R0 from transmission experiments. By using previously published data of experiments with Classical Swine Fever Virus more extensively, we obtained smaller confidence intervals than the martingale method used in the original papers. Moreover, our method allows simultaneous estimation of a reproduction ratio within pens R0w and a modified reproduction ratio between pens R'0b. Resulting estimates of R0w and R'0b for weaner pigs were 100 (95% CI 54.4-186) and 7.77 (4.68-12.9), respectively. For slaughter pigs they were 15.5 (6.20-38.7) and 3.39 (1.54-7.45), respectively. We believe, because of the smaller confidence intervals we were able to obtain, that the method presented here is better suited for use in future experiments

Sustainable Soesterkwartier

The municipality of Amersfoort wants to construct an endurable and sustainable eco-town in the Soesterkwartier neighbourhood, by taking future climate change into account. The impact of climate change at the location of the proposed eco-town was studied by a literature review

Intelligent multimedia indexing and retrieval through multi-source information extraction and merging

This paper reports work on automated meta-data\ud creation for multimedia content. The approach results\ud in the generation of a conceptual index of\ud the content which may then be searched via semantic\ud categories instead of keywords. The novelty\ud of the work is to exploit multiple sources of\ud information relating to video content (in this case\ud the rich range of sources covering important sports\ud events). News, commentaries and web reports covering\ud international football games in multiple languages\ud and multiple modalities is analysed and the\ud resultant data merged. This merging process leads\ud to increased accuracy relative to individual sources

Structure of Disk Dominated Galaxies I. Bulge/Disk Parameters, Simulations, and Secular Evolution

(Abridged) A robust analysis of galaxy structural parameters, based on the modeling of bulge and disk brightnesses in the BVRH bandpasses, is presented for 121 face-on and moderately inclined late-type spirals. Each surface brightness (SB) profile is decomposed into a sum of a generalized Sersic bulge and an exponential disk. The reliability and limitations of our bulge-to-disk (B/D) decompositions are tested with extensive simulations of galaxy brightness profiles (1D) and images (2D). Galaxy types are divided into 3 classes according to their SB profile shapes; Freeman Type-I and Type-II, and a third ``Transition'' class for galaxies whose profiles change from Type-II in the optical to Type-I in the infrared. We discuss possible interpretations of Freeman Type-II profiles. The Sersic bulge shape parameter for nearby Type-I late-type spirals shows a range between n=0.1-2 but, on average, the underlying surface density profile for the bulge and disk of these galaxies is adequately described by a double-exponential distribution. We confirm a coupling between the bulge and disk with a scale length ratio r_e/h=0.22+/-0.09, or h_bulge/h_disk=0.13+/-0.06 for late-type spirals, in agreement with recent N-body simulations of disk formation and models of secular evolution. This ratio increases from ~0.20 for late-type spirals to ~0.24 for earlier types. The similar scaling relations for early and late-type spirals suggest comparable formation and/or evolution scenarios for disk galaxies of all Hubble types.Comment: 78 pages with 23 embedded color figures + tables of galaxy structural parameters. Accepted for publication in the Astrophysical Journal. The interested reader is strongly encouraged to ignore some of the low res figures within; instead, download the high resolution version from http://www.astro.ubc.ca/people/courteau/public/macarthur02_disks.ps.g

Dynamical Expansion of Ionization and Dissociation Front around a Massive Star. II. On the Generality of Triggered Star Formation

We analyze the dynamical expansion of the HII region, photodissociation region, and the swept-up shell, solving the UV- and FUV-radiative transfer, the thermal and chemical processes in the time-dependent hydrodynamics code. Following our previous paper, we investigate the time evolutions with various ambient number densities and central stars. Our calculations show that basic evolution is qualitatively similar among our models with different parameters. The molecular gas is finally accumulated in the shell, and the gravitational fragmentation of the shell is generally expected. The quantitative differences among models are well understood with analytic scaling relations. The detailed physical and chemical structure of the shell is mainly determined by the incident FUV flux and the column density of the shell, which also follow the scaling relations. The time of shell-fragmentation, and the mass of the gathered molecular gas are sensitive tothe ambient number density. In the case of the lower number density, the shell-fragmentation occurs over a longer timescale, and the accumulated molecular gas is more massive. The variations with different central stars are more moderate. The time of the shell-fragmentation differs by a factor of several with the various stars of M_* = 12-101 M_sun. According to our numerical results, we conclude that the expanding HII region should be an efficient trigger for star formation in molecular clouds if the mass of the ambient molecular material is large enough.Comment: 49 pages, including 17 figures ; Accepted for publication in Ap

Split-gate quantum point contacts with tunable channel length

We report on developing split-gate quantum point contacts (QPCs) that have a tunable length for the transport channel. The QPCs were realized in a GaAs/AlGaAs heterostructure with a two- dimensional electron gas (2DEG) below its surface. The conventional design uses 2 gate fingers on the wafer surface which deplete the 2DEG underneath when a negative gate voltage is applied, and this allows for tuning the width of the QPC channel. Our design has 6 gate fingers and this provides additional control over the form of the electrostatic potential that defines the channel. Our study is based on electrostatic simulations and experiments and the results show that we developed QPCs where the effective channel length can be tuned from about 200 nm to 600 nm. Length-tunable QPCs are important for studies of electron many-body effects because these phenomena show a nanoscale dependence on the dimensions of the QPC channel

Self-Regulation of Star Formation in Low Metallicity Clouds

We investigate the process of self-regulated star formation via photodissociation of hydrogen molecules in low metallicity clouds. We evaluate the influence region's scale of a massive star in low metallicity gas clouds whose temperatures are between 100 and 10000 Kelvin. A single O star can photodissociate hydrogen molecules in the whole of the host cloud. If metallicity is smaller than about 10^{-2.5} of the solar metallicity, the depletion of coolant of the the host cloud is very serious so that the cloud cannot cool in a free-fall time, and subsequent star formation is almost quenched. On the contrary, if metallicity is larger than about 10^{-1.5} of the solar metallicity, star formation regulation via photodissociation is not efficient. The typical metallicity when this transition occurs is about 1/100 of the solar metallicity. This indicates that stars do not form efficiently before the metallicity becomes larger than about 1/100 of the solar metallicity and we considered that this value becomes the lower limit of the metallicity of luminous objects such as galaxies.Comment: 14 pages, including 5 figures, To appear in ApJ, Vol. 53

Stabilizing nuclear spins around semiconductor electrons via the interplay of optical coherent population trapping and dynamic nuclear polarization

We experimentally demonstrate how coherent population trapping (CPT) for donor-bound electron spins in GaAs results in autonomous feedback that prepares stabilized states for the spin polarization of nuclei around the electrons. CPT was realized by excitation with two lasers to a bound-exciton state. Transmission studies of the spectral CPT feature on an ensemble of electrons directly reveal the statistical distribution of prepared nuclear spin states. Tuning the laser driving from blue to red detuned drives a transition from one to two stable states. Our results have importance for ongoing research on schemes for dynamic nuclear spin polarization, the central spin problem and control of spin coherence.Comment: 5 pages, 4 figure

Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes

Epitope-tagged active-site-directed probes are widely used to visualize the activity of deubiquitinases (DUBs) in cell extracts, to investigate the specificity and potency of small-molecule DUB inhibitors, and to isolate and identify DUBs by mass spectrometry. With DUBs arising as novel potential drug targets, probes are required that can be produced in sufficient amounts and to meet the specific needs of a given experiment. The established method for the generation of DUB probes makes use of labor-intensive intein-based methods that have inherent limitations concerning the incorporation of unnatural amino acids and the amount of material that can be obtained. Here, we describe the total chemical synthesis of active-site-directed probes and their application to activity-based profiling and identification of functional DUBs. This synthetic methodology allowed the easy incorporation of desired tags for specific applications, for example, fluorescent reporters, handles for immunoprecipitation or affinity pull-down, and cleavable linkers. Additionally, the synthetic method can be scaled up to provide significant amounts of probe. Fluorescent ubiquitin probes allowed faster, in-gel detection of active DUBs, as compared to (immuno)blotting procedures. A biotinylated probe holding a photocleavable linker enabled the affinity pull-down and subsequent mild, photorelease of DUBs. Also, DUB activity levels were monitored in response to overexpression or knockdown, and to inhibition by small molecules. Furthermore, fluorescent probes revealed differential DUB activity profiles in a panel of lung and prostate cancer cells