De Jong, D.C. D.

Montgomery, F. H.

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D.C. D. De Jong

F. H. Montgomery

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Aliso

Chromosome Numbers in Some Californian Compositae-astereae

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Aliso: A Journal of Systematic and Evolutionary BotanyVolume 5 | Issue 3 Article 51963Chromosome Numbers in Some CalifornianCompositae-astereaeD.C. D. De JongMichigan State University, Ontario Agricultural CollegeF. H. MontgomeryMichigan State University, Ontario Agricultural CollegeFollow this and additional works at: http://scholarship.claremont.edu/alisoPart of the Botany CommonsRecommended CitationDe Jong, D.C. D. and Montgomery, F. H. (1963) "Chromosome Numbers in Some Californian Compositae-astereae," Aliso: A Journalof Systematic and Evolutionary Botany: Vol. 5: Iss. 3, Article 5.Available at: http://scholarship.claremont.edu/aliso/vol5/iss3/5ALISO VoL. 5, No.3, pp. 255-256 CHROMOSOME NUMBERS IN SOME CALIFORNIAN COMPOSIT AE-ASTEREAE D. C. D. DEJoNG AND F. H. MoNTGOMERY Department of Botany and Plant Pathology, Michigan State University, Ea.rt Lansing, Michigan and Department of Botany, Ontario Agricultural College, Guelph, Ontario, Canada APRIL 15, 1963 The present chromosome counts were made from plants grown from seeds kindly supplied by Rancho Santa Ana Botanic Garden, Claremont, California. Table 1 lists 16 original counts, 11 of which are from taxa here reported for the first time. The vouchers cited in the table are in the Herbarium of the Garden and are specimens collected at the time the seeds were originally obtained. In some cases the plants from which the counts were made were several generations removed from the original collections, but hybridization was not sus-pected in any of these. Root tips were collected from potted plants in the greenhouse and pretreated in a saturated solution of paradichlorobenzene for 2-3 hrs at 15 ° C and fixed in a Carnoy solution ( 3 parts absolute ethyl alcohol : 1 part glacial acetic acid) for 20 minutes at room temperature. They were then hydrolyzed inN HCl at 60° C for 3-5 minutes and stained with aceta-orcein. The squash technique was employed in making the slides. Slides were dehydrated in a series of absolute ethyl alcohol, absolute ethyl alcohol and xylol 1 : 1, and xylol and made perma-nent using DPX as the mounting medium. Permanent slides are in the collection of the Department of Botany, Ontario Agricultural College, and unpublished photomicrographs are in the possession of the senior author. The authors are greatly indebted to Dr. Peter H. Raven of the Rancho Santa Ana Botanic Garden for aid during the preparation of the manuscript and for verifying the identification of the voucher specimens. DISCUSSION The chromosome counts for Grindelia camporttm, Haplopappus arborescens, H. venettt.r var. vernonioides, Heterotheca grandiflora, and Monoptilon bellioides are in agreement with those reported by Raven eta!. (Am. J. Botan. 47: 124-132, 1960) for plants from different localities in California. At the generic level, the counts for Baccharis, Chrysothamnus, Corethrogyne, and Grindelia are consistent with the basic chromosome numbers previously reported for these genera ( cf. Raven eta!., op. cit.). It is interesting that all counts published to date for the predominently woody genus Baccharis are diploid, as are the new counts here reported for B. sarothroides and B. sergilioides. Our count for B. pilularis subsp. pilularis agrees with that of Raven eta!. ( op. cit.) for subsp. consangttinea. The chromosome number of the typical variety of Corethrogyne filaginifolia is the same as those previously published for other varieties of that species. [255] 256 ALISO [VoL. S, No. 3 The counts for Haplopappu.r .rquarroms subsp. squarrosus agree with those of Raven et al. for subsp. grindelioides from southern California. It is significant that our count of H. acradenius subsp. eremophilus from near Victorville is diploid (2n=l2) while these authors reported H. acradenius subsp. acradenius from a few miles east to be tetraploid ( 2n=24). Judging from the meiotic configurations, Raven (in !itt.) considers that his strain of H. acradenius subsp. acradenius might have been autotetraploid. The new counts for H. ericoides and H. parishii lend support to the hypothesis of 9 as the basic chromosome number of the shrubby section Ericameria which, on morphological grounds, is closely related to the shrubby genus Chrysothamnus, which has also x=9. TABLE 1. Chromosome numbers in Compositae-Astereae. NAME SOMATIC CHROMOSOME NUMBER Baccharis pilularis DC. subsp. pilularis sarothroides Gray serf!,ilioides Gray Chrysothamnus fJaniculatus (Gray) Hall Corethrogyne filaf!,inifolia (H. & A.) Nutt. var. filaf!,inifolia Grindelia camporum Greene latifolia Kell. stricta subsp. t'enulosa (Jeps.) Keck Haplopappus acradenius subsp. eremophilus (Greene) Hall arborescens (Gray) Hall ericoide.r (Less.) H. & A. paris hi i (Greene) Blake squarrosus H. & A. subsp. squarrosus venetus (Kunth) Blake var. vernonioides (Nutt.) Munz Heterotheca f!.randiflora Nutt. Monoptilon bellioides (Gray) Hall 18* 18* 18* 18* 10* 24 24* 24* 12* 18 18* 18* 10* 12 18 16 LOCALITY AND COLLECTION 4 miles south of Pescadero, San Mateo Co., Wolf 1807 S miles east of Holtville, Imperial Co., Wolf 9391 Little San Bernardino Mts., Riverside Co., Wolf 4297 Morongo Valley, San Bernardino Co., Balls & Everett 22802 1.5 miles south of Pismo Beach, San Luis Obispo Co., Everett & Balls 18701 1.3 miles north of Sonora, Toulumne Co., Balls & Everett 18082 Santa Cruz I., Santa Barbara Co., Balls & Blakley 23743 Pt. Reyes Lighthouse, Marin Co., Balls 23578 North of Victorville, San Bernardino Co., Balls 22207 5.3 miles northwest of Sonora, Tuo-lumne Co., Balls & Everett 18084 Hurricane Pt., Monterey Co., EtJetett & Balls 18695 Near Julian, San Diego Co., Munz 12250 Carmel Highlands, Monterey Co., Everett & Balls 18694 2 miles south of Piedras Blancas, San Luis Obispo Co., Everett & Balls 18698 Santa Cruz I., Santa Barbara Co., Balls & Blakley 23632 U. S. Highway 60-70, 5 miles east of junction with U. S. 99, Riverside Co., Everett & Balls 22959 *The chromosome counts marked with an asterisk are reported for the first time. 

Keck Graduate Institute

Aliso: A Journal of Systematic and Evolutionary Botany
Volume 5 | Issue 3 Article 5
1963
Chromosome Numbers in Some Californian
Compositae-astereae
D.C. D. De Jong
Michigan State University, Ontario Agricultural College
F. H. Montgomery
Michigan State University, Ontario Agricultural College
Follow this and additional works at: http://scholarship.claremont.edu/aliso
Part of the Botany Commons
Recommended Citation
De Jong, D.C. D. and Montgomery, F. H. (1963) "Chromosome Numbers in Some Californian Compositae-astereae," Aliso: A Journal
of Systematic and Evolutionary Botany: Vol. 5: Iss. 3, Article 5.
Available at: http://scholarship.claremont.edu/aliso/vol5/iss3/5
ALISO 
VoL. 5, No.3, pp. 255-256 
CHROMOSOME NUMBERS IN SOME 
CALIFORNIAN COMPOSIT AE-ASTEREAE 
D. C. D. DEJoNG AND F. H. MoNTGOMERY 
Department of Botany and Plant Pathology, 
Michigan State University, Ea.rt Lansing, Michigan 
and 
Department of Botany, Ontario Agricultural College, 
Guelph, Ontario, Canada 
APRIL 15, 1963 
The present chromosome counts were made from plants grown from seeds kindly supplied 
by Rancho Santa Ana Botanic Garden, Claremont, California. Table 1 lists 16 original 
counts, 11 of which are from taxa here reported for the first time. The vouchers cited in the 
table are in the Herbarium of the Garden and are specimens collected at the time the seeds 
were originally obtained. In some cases the plants from which the counts were made were 
several generations removed from the original collections, but hybridization was not sus-
pected in any of these. 
Root tips were collected from potted plants in the greenhouse and pretreated in a saturated 
solution of paradichlorobenzene for 2-3 hrs at 15 ° C and fixed in a Carnoy solution ( 3 parts 
absolute ethyl alcohol : 1 part glacial acetic acid) for 20 minutes at room temperature. They 
were then hydrolyzed inN HCl at 60° C for 3-5 minutes and stained with aceta-orcein. 
The squash technique was employed in making the slides. Slides were dehydrated in a series 
of absolute ethyl alcohol, absolute ethyl alcohol and xylol 1 : 1, and xylol and made perma-
nent using DPX as the mounting medium. Permanent slides are in the collection of the 
Department of Botany, Ontario Agricultural College, and unpublished photomicrographs 
are in the possession of the senior author. 
The authors are greatly indebted to Dr. Peter H. Raven of the Rancho Santa Ana Botanic 
Garden for aid during the preparation of the manuscript and for verifying the identification 
of the voucher specimens. 
DISCUSSION 
The chromosome counts for Grindelia camporttm, Haplopappus arborescens, H. venettt.r 
var. vernonioides, Heterotheca grandiflora, and Monoptilon bellioides are in agreement with 
those reported by Raven eta!. (Am. J. Botan. 47: 124-132, 1960) for plants from different 
localities in California. At the generic level, the counts for Baccharis, Chrysothamnus, 
Corethrogyne, and Grindelia are consistent with the basic chromosome numbers previously 
reported for these genera ( cf. Raven eta!., op. cit.). It is interesting that all counts published 
to date for the predominently woody genus Baccharis are diploid, as are the new counts here 
reported for B. sarothroides and B. sergilioides. Our count for B. pilularis subsp. pilularis 
agrees with that of Raven eta!. ( op. cit.) for subsp. consangttinea. The chromosome number 
of the typical variety of Corethrogyne filaginifolia is the same as those previously published 
for other varieties of that species. 
[255] 
256 ALISO [VoL. S, No. 3 
The counts for Haplopappu.r .rquarroms subsp. squarrosus agree with those of Raven et al. 
for subsp. grindelioides from southern California. It is significant that our count of H. 
acradenius subsp. eremophilus from near Victorville is diploid (2n=l2) while these authors 
reported H. acradenius subsp. acradenius from a few miles east to be tetraploid ( 2n=24). 
Judging from the meiotic configurations, Raven (in !itt.) considers that his strain of H. 
acradenius subsp. acradenius might have been autotetraploid. The new counts for H. 
ericoides and H. parishii lend support to the hypothesis of 9 as the basic chromosome number 
of the shrubby section Ericameria which, on morphological grounds, is closely related to the 
shrubby genus Chrysothamnus, which has also x=9. 
TABLE 1. Chromosome numbers in Compositae-Astereae. 
NAME 
SOMATIC CHROMOSOME 
NUMBER 
Baccharis pilularis DC. 
subsp. pilularis 
sarothroides Gray 
serf!,ilioides Gray 
Chrysothamnus fJaniculatus (Gray) Hall 
Corethrogyne filaf!,inifolia (H. & A.) Nutt. 
var. filaf!,inifolia 
Grindelia camporum Greene 
latifolia Kell. 
stricta subsp. t'enulosa (Jeps.) Keck 
Haplopappus acradenius subsp. 
eremophilus (Greene) Hall 
arborescens (Gray) Hall 
ericoide.r (Less.) H. & A. 
paris hi i (Greene) Blake 
squarrosus H. & A. subsp. squarrosus 
venetus (Kunth) Blake 
var. vernonioides (Nutt.) Munz 
Heterotheca f!.randiflora Nutt. 
Monoptilon bellioides (Gray) Hall 
18* 
18* 
18* 
18* 
10* 
24 
24* 
24* 
12* 
18 
18* 
18* 
10* 
12 
18 
16 
LOCALITY AND COLLECTION 
4 miles south of Pescadero, San Mateo 
Co., Wolf 1807 
S miles east of Holtville, Imperial Co., 
Wolf 9391 
Little San Bernardino Mts., Riverside 
Co., Wolf 4297 
Morongo Valley, San Bernardino Co., 
Balls & Everett 22802 
1.5 miles south of Pismo Beach, San 
Luis Obispo Co., Everett & Balls 18701 
1.3 miles north of Sonora, Toulumne 
Co., Balls & Everett 18082 
Santa Cruz I., Santa Barbara Co., Balls 
& Blakley 23743 
Pt. Reyes Lighthouse, Marin Co., Balls 
23578 
North of Victorville, San Bernardino 
Co., Balls 22207 
5.3 miles northwest of Sonora, Tuo-
lumne Co., Balls & Everett 18084 
Hurricane Pt., Monterey Co., EtJetett 
& Balls 18695 
Near Julian, San Diego Co., Munz 
12250 
Carmel Highlands, Monterey Co., 
Everett & Balls 18694 
2 miles south of Piedras Blancas, San 
Luis Obispo Co., Everett & Balls 18698 
Santa Cruz I., Santa Barbara Co., Balls 
& Blakley 23632 
U. S. Highway 60-70, 5 miles east of 
junction with U. S. 99, Riverside Co., 
Everett & Balls 22959 
*The chromosome counts marked with an asterisk are reported for the first time. 


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Chromosome Numbers in Some Californian Compositae-astereae

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