Comparative Analysis of Viperidae Venoms Antibacterial Profile: a Short Communication for Proteomics

Bacterial infections involving multidrug-resistant strains are one of the ten leading causes of death and an important health problem in need for new antibacterial sources and agents. Herein, we tested and compared four snake venoms (Agkistrodon rhodostoma, Bothrops jararaca, B. atrox and Lachesis muta) against 10 Gram-positive and Gram-negative drug-resistant clinical bacteria strains to identify them as new sources of potential antibacterial molecules. Our data revealed that, as efficient as some antibiotics currently on the market (minimal inhibitory concentration (MIC) = 1–32 μg mL−1), A. rhodostoma and B. atrox venoms were active against Staphylococcus epidermidis and Enterococcus faecalis (MIC = 4.5 μg mL−1), while B. jararaca inhibited S. aureus growth (MIC = 13 μg ml−1). As genomic and proteomic technologies are improving and developing rapidly, our results suggested that A. rhodostoma, B. atrox and B. jararaca venoms and glands are feasible sources for searching antimicrobial prototypes for future design new antibiotics against drug-resistant clinical bacteria. They also point to an additional perspective to fully identify the pharmacological potential of these venoms by using different techniques

Essential Oil of Croton ceanothifolius Baill. Potentiates the Effect of Antibiotics against Multiresistant Bacteria

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2020-02-07T16:46:41Z No. of bitstreams: 1 Araújo, Essential....pdf: 480059 bytes, checksum: fae6482a0731dd97886ba0302a211c87 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2020-02-07T16:59:24Z (GMT) No. of bitstreams: 1 Araújo, Essential....pdf: 480059 bytes, checksum: fae6482a0731dd97886ba0302a211c87 (MD5)Made available in DSpace on 2020-02-07T16:59:24Z (GMT). No. of bitstreams: 1 Araújo, Essential....pdf: 480059 bytes, checksum: fae6482a0731dd97886ba0302a211c87 (MD5) Previous issue date: 2020-01-14Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.Federal University of Paraná. Curitiba, PR, Brazil.Federal University of Paraná. Curitiba, PR, Brazil.Federal University of Paraná. Curitiba, PR, Brazil.Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.Milan State University. Department of Agricultural and Environmental Sciences. Milan, Italy.Regional University of Cariri. Department of Biological Chemistry. Crato, CE, Brazil.This study is a pioneer in reporting the antibacterial properties of the species Croton ceanothifolius Baill. The genus Croton belongs to the family Euphorbiaceae composed of numerous species with documented biological activities. However, the pharmacological properties of C. ceanothifolius remain poorly understood. The leaves of this plant were submitted to hydrodistillation for essential oil (CcEO) extraction and the phytochemical characterization of the oil was performed by GC/MS. The minimum inhibitory concentration of the CcEO was determined for the evaluation of antibacterial activity against multiresistant strains of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The antibiotic-modulating activity of the oil, in combination with antibiotics, was also evaluated. The combination of the CcEO with penicillin, norfloxacin, and gentamicin presented a synergistic effect. This effect was more significant for the association with antibiotics of the quinolone and aminoglycoside classes against Escherichia coli. The association of oil with gentamicin showed better results with regard to the Gram-positive strain. The association of the oil with norfloxacin against P. aeruginosa also showed synergism, but the association with penicillin did not change the effect of this antibiotic. Thus, it is concluded that C. ceanothifolius essential oil selectively potentiates the action of antibiotics against multiresistant strains

Propriedades físico-hídricas em Latossolo do Cerrado sob diferentes sistemas de manejo Physicohydric properties of Cerrado Latosol under different management systems

Visou-se, neste trabalho, avaliar as propriedades físico-hídricas do solo sob diferentes sistemas de manejo, em comparação com o Cerrado nativo, em Santo Antônio de Goiás, GO, em áreas cultivadas desde o ano de 1993. As propriedades físico-hídricas foram avaliadas nas profundidades de 0-0,10 e 0,10-0,20 m do solo submetido aos sistemas de manejo: S1 (soja/feijão/arroz/milheto/feijão), S2 (soja/milheto/milho), S3 (milho + pastagem de braquiária - integração-lavoura-pecuária) e S4 (cerrado nativo). Utilizou-se, para comparar os resultados obtidos nos diversos sistemas de manejo do solo, o intervalo de confiança com nível de probabilidade de 5%. A área com pastagem de braquiária sob pastejo animal promoveu compactação do solo na camada superficial, verificada pela redução da macroporosidade, aumento da microporosidade e da densidade do solo.<br>The objective of this study was to evaluate the physico-hydric soil properties under different management systems in comparison with the native Cerrado, in Santo Antônio de Goiás, Goiás State, in areas cultivated since 1993. The evaluated systems in the depths of 0 - 0.10 and 0.10 - 0.20 m were: system S1 (soybean/bean/rice/pearl millet /bean), system S2 (soybean/pearl millet/corn), system S3 (corn + Brachiaria brizantha in integrated crop-livestock system) and system S4 (native cerrado). To compare the results obtained in the several soil management systems, the interval of confidence was used, at a probability level of 5%. The use of area under Brachiaria brizantha pasture for animal grazing caused compaction of the soil in the surface layer, verified through the reduction of the macroporosity, and increase of the microporosity and soil bulk density