Métodos preventivos contra agentes contaminantes em cultura de células primárias de Membrana Sinovial Caprina.

Objetivou-se com este estudo descrever métodos preventivos contra contaminação em cultura celular primária de Membrana Sinovial Caprina (MSC) adotados no Laboratório de Virologia da Embrapa Caprinos e Ovinos, Sobral-CE, e demonstrar a sua importância dentro de um laboratório de cultura de células

Starch accumulation and agronomical performance of Syrah under winter cycle: responses to pruning and ethephon management

In the Brazilian Southeast, 'Syrah' grape harvested during the winter reaches better quality index than those from summer harvest. However, the management of annual double pruning to produce two grapevine growth cycles (vegetative cycle: spring – summer; reproductive cycle: autumn – winter) has increased the vineyard production costs and the vine reserve accumulation can be compromised. This study had two main objectives to improve the winter cultivation: i) to validate single pruning carried out only in summer (February) (experiment 1); to increase the reserve accumulation by ethephon (ethrel) sprayed two months before yield pruning (experiment 2). Both experiments were carried out in south of Minas Gerais State using three years old field-grown 'Syrah' grapevines grafted onto '1103 Paulsen' and trained in a vertical shoot position. The results from experiment 1 showed that grapevines pruned in summer also need to be pruned during the winter after grape harvest to avoid bud infertility during the following autumn-winter season. Single pruning reduced the starch contents in shoots, trunks and roots sampled before yield pruning and the commercial grape harvest was completely unavailable due to unfruitful shoots. On the other hand, the autumn-winter cycle was improved by ethephon sprayed in the previous vegetative growing cycle (summer cycle). During the autumn-winter cycle, sprayed grapevines showed higher starch content in trunks, high vegetative vigor and the yield and grape quality were not negatively affected

Bone grafts and substitutes in small animal orthopaedic reconstrutive surgery: a breaf review

We present here an overview of the scientific literature concerning the clinical application of autografts and allografts of the skeletal tissues and of the natural and synthetic bone graft substitutes available in small animal orthopedic surgery for resolution of congenital, traumatic and tumoral etiology situations or for reconstructive artroplastic surgeries. This article focuses the terminology, definitions and characteristics of the bone grafts and bone graft substitutes based in their functions and composition, the process of incorporation of the bone grafts and bone graft substitutes, the collection of autogenous cancellous or corticocancellous bone for grafting, the available commercial forms of bone graft or bone graft substitutes and the biologic/synthetic composite grafts, other accessible options in small animal orthopedic surgery with osteogenic and/ or osteoindutive characteristics (ex. collection and use of the autogenous bone marrow or platelet rich plasma), the principal clinical indications and surgical techniques in which are recommended the use of bone graft and/or bone graft substitutes and how to select the best option for each specific clinical case. At last, the future perspectives in reconstructive orthopedic surgery are also pointed, namely the tissue engineering of bone and cartilage tissues and the medical three dimensional biomodulation.Neste artigo pretendeu realizar-se uma revisão da literatura científica da informação disponível sobre os vários tipos de auto- e aloenxertos do sistema musculo-esquelético e substitutos ósseos naturais e sintéticos do tecido ósseo para utilização em cirurgia ortopédica dos animais de companhia com vista à resolução cirúrgica de situações clínicas do foro da traumatologia e ortopedia de etiologia congénita, traumática, tumoral ou em implantações artroplásticas. Abordam-se assim os conceitos gerais sobre a terminologia, definições e classificação dos enxertos e substitutos ósseos com base nas suas funções e composição, o processo de incorporação dos enxertos e substitutos ósseos, os principais locais de obtenção e a correta técnica de transplante de enxertos ósseos autógenos, os principais tipos de enxertos e substitutos ósseos comercializados e os enxertos compostos, outras abordagens autógenas acessíveis em animais de companhia com potencial osteogénico e/ou osteoindutor (ex. obtenção e utilização de medula óssea autógena ou plasma enriquecido em plaquetas), as principais situações clínicas e técnicas cirúrgicas em que se recomenda a utilização de enxertos e/ou substitutos ósseos e como selecionar em cada caso clínico a melhor opção disponível. Por fim, são também abordadas as perspetivas futuras em cirurgia ortopédica reconstrutiva, nomeadamente a engenharia do tecido ósseo e cartilagem e a biomodelação tridimencional médica

An update on the occurrence and damage caused to cashew by Gypona cerea DeLong & Freytag (Hemiptera: Cicadellidae) in Northeastern Brazil.

Made available in DSpace on 2020-01-09T18:13:00Z (GMT). No. of bitstreams: 1 ART19087.pdf: 590683 bytes, checksum: bf73465460d2a60da4face66cca19e5c (MD5) Previous issue date: 2019bitstream/item/208416/1/ART19087.pdf; bitstream/item/208416/1/ART19087.pd

Normas DRIS preliminares para pupunheira cultivada em diferentes sistemas de manejo na região Sul-ocidental da Amazônia.

A pupunheira, uma frutífera nativa da América Central e da Floresta Amazônica, vem sendo cultivada para produção de frutos e extração de palmito

Evaluation of bone turnover markers and serum minerals variations for predicting fracture healing versus non-union processes in adult sheep as a model for orthopedic research

Bone turnover markers (BTMs) have been considered as an auxiliary method of following the fracture healing process and for early prediction of impaired bone healing. A better understanding of the potential of BTMs in this application could allow for earlier interventions and improved patient care. The aim of this study with a large animal experimental model was to assess the variation of bone formation markers SOH namely the total alkaline phosphatase (ALP) and its bone-specific isoform (BALP), serum concentration of intact osteocalcin (OC), N-terminal propeptide type III procollagen (PIIINP) and of bone resorption markers â  namely tartrate resistant acid phosphatase (TRAP) and deoxypyridinoline crosslink (DPD) during the first stages of a normal fracture healing process and of a segmental critical size defect (CSD), which progresses to a non-union process. Thirty healthy female sheep (Portuguese Churra-da-Terra- Quente breed), approximately 4-years-old, were enrolled in this study. Jugular venous blood samples were collected pre-operatively and at 1, 2, 3, 4, 6, 8, 10 and 12 post-operative weeks. The animals of the CSD group showed significant lower serum levels of BALP, OC and significant higher serum PIIINP levels at early stages of the fracture healing process, compared with animals that progressed in a normal fracture healing process. Serum BALP, OC and PIIINP levels could be useful as non-invasive auxiliary tools with other complementary methods for predicting the outcome of traumatic bone fractures.Cristina P. Sousa acknowledges the Portuguese Foundation for Science and Technology (FCT) for her PhD scholarship (Grant No SFRH/BD/45018/2008).info:eu-repo/semantics/publishedVersio

Gene banks that promote on-farm management through the reintroduction of local varieties in Brazil.

bitstream/item/155836/1/2.5.genebanks-reintroduce-local-varieties-Brazilpdf.pd

Are stomatal area and stomatal density reliable traits for identification of doubled haploids in maize?

To meet the demands of the production chain, breeding programs need to reduce the time for selection and development of higher yielding maize genotypes. This involves application of new strategies, such as the use of the doubled haploid lines. We evaluated the use of stomatal area and density in distinguishing doubled haploids (DH) from false positive (FP) plants derived from nine tropical source populations. We selected four DH plants and two FP plants for identification of DH through leaf anatomy in the populations, in each group. Data on stomatal area and density were considered in a completely randomized model in a factorial scheme (populations and genotypes) and analyzed by restricted maximum likelihood. There were statistically significant effects of populations and genotypes for both traits. For stomatal density, the population by genotype interaction was not verified. Stomatal density proved to be efficient for distinguishing DH and FP for use in selection of doubled haploids