Optimisation of a one-step PCR assay for the diagnosis of Flavescence doreerelated phytoplasmas in field-grown grapevines and vector populations

Field-infected grapevines and natural populations of Scaphoideus titanus have been analysed to detect group V phytoplasmas associated with flavescence doree in northwestern Italy using nested PCR. A first amplification driven by universal ribosomal primers R16SF2/R2 was followed by a second round assisted by R16(V)F1/R1 primers and subsequent RFLP analysis. To optimize the test, nested PCRs were compared with direct amplification assisted by the group V-specific fAY/rEY primer pair, directed towards other ribosomal sequences. In nested and direct PCRs, respectively, DNAs from 71 and 57 out of 96 grapevines (i.e. 73.9 and 59.3 %) and 51 and 50 out of 108 insects (i.e. 47.2 and 46.3 %) reacted positively. Although it was not possible to determine the subgroup of the phytoplasmas after fAY/rEY amplification, these primers could be used successfully in mass screening of plant material and insect populations. They could detect, in single-step amplification, the phytoplasmas in 80 and 98 % of the plant and insect samples, respectively, that were already indexed as positive using nested PCR. This strongly reduced the number of samples requiring the nested approach, with beneficial effects on costs, labour and risks of the analysis.

Application of Surrogate Models for Building Envelope Design Exploration and Optimization

Building performance simulations are usually timeconsuming. They may account for the major portion of time spent in Computational Design Optimization (CDO), for instance, annual hourly daylight and energy simulations. In this case, the optimization may become less efficient or even infeasible within a limited time frame of real-world projects, due to the computationally expensive simulations. To handle the problem, this research aims to investigate the potentials of surrogate models (i.e. Response SurfaceMethodology - RSM) to be used in the building envelope design exploration and optimization that consider visual and energy performance. Specifically, the work investigates how, and to what extent, 1) problem scales may affect the application of RSM, and 2) different ways of using RSM may affect the quality of Pareto Front approximations. Thus, a series of multi-objective optimization tests are carried out; preliminary discussion is made based on the current results.Design Informatic

Simulating natural ventilation in large sports buildings: Prediction of temperature and airflow patterns in the early design stages

In large sport’s buildings, a big part of energy can be saved by providing natural instead of mechanical ventilation. However, additional challenges arise while controlling airflow and temperatures in different zones. These measureshighly depend on the shape, construction and ventilation openings, which are mostly decided in the early design stages. Computational optimization can support these early stages of design, but needs to be performed in efficient ways. In this respect, the project proposes rapid assessment of temperature and airflow patterns using customized Grasshopper components, which would be able to evaluate a given model using CONTAM and EnergyPlus software assimulation engine. The proposed method integrates these simulations within an environment, which is familiar to architects and is largely used for parameterization of design in its early stages. A case study (Jiangmen Sports Center, Jiangmen, China) is used to test the developed process for a large indoor sports hall.Design Informatic

Endomorphin-1 prevents lipid accumulation via CD36 down-regulation and modulates cytokines release from human lipid-laden macrophages

CD36 is a scavenger receptor known to play a critical role in the development of atherosclerosis by mediating the uptake of oxidized low-density lipoproteins (oxLDL) by macrophages. thus leading to foam cell formation. It is now generally recognized that the immune system has a pivotal role in the pathogenesis of atherosclerosis, whose progression is determined by ongoing inflammatory reactions. Recently, several studies pointed out that opioid peptides exert anti-inflammatory activities. Therefore the aim of the present study was to evaluate a possible endomorphin-1 (EM-1) immunomodulatory activity on human foam cells. Our results showed that EM-1 reduced Nile Red-stained lipid droplets content, decreased the expression of CD36 receptor and modulated tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) release from lipid-laden macrophages. Furthermore, Naloxone, an opioid receptors antagonist, reverted the anti-atherogenic and anti-inflammatory observed effects of EM-1. These data demonstrated, for the first time, an unprecedented ability of EM-1 to act as a novel modulator for macrophage-to-foam cell transformation, and for inflammatory cytokines profile, suggesting possible novel endomorphin-based anti-atherosclerotic approaches for the prevention and treatment of atherosclerosis. (C) 2010 Elsevier Inc. All rights reserved

Activatory properties of lysophosphatidic acid on human THP-1 cells

Excessive leukocyte proliferation and proinflammatory mediators release represent common phenomena in several chronic inflammatory diseases. Multiple evidences identify lysophosphatidic acid (LPA), a small lipid endowed with pleiotropic activities, as an important modulator of both proliferation and activation of different cell types involved in several inflammation-associated pathologies. However, its possible role on monocyte proinflammatory activation is not fully understood yet. Aim of the present study was to investigate LPA effects on THP-1 cells in terms of proliferation, reactive oxygen intermediates (ROI) production and release of arachidonic acid-derived inflammatory mediators. Actually, LPA significantly increased both DNA synthesis and ROI production as well as prostaglandin E-2 release and the upregulation of LPA(3) receptor expression. These findings identified LPA as both a growth factor and a triggering mediator of proinflammatory response in THP-1 cells

Computational Design for Sport Building

The design of sport buildings has great impact on top-sport as well as on recreational sport-activities. It implies challenging tasks in meeting the performance-requirements. This includes the control of factors like daylight/lighting, air flow, thermal conditions, just to name a few. Such factors impact the performance of athletes and are hard to control in large sport halls; their control is even harder when the public/audience is located within the halls and require different climate conditions. While mechanical installations are often needed during competitions in order to guarantee constant conditions, relaying on mechanical installations during the daily and recreational use of the venues challenges their medium/long term sustainability. Computational form finding approaches can favour the achievement of high-performing and sustainable sport buildings. In this light, the paper tackles the use of Multi-objective and Multidisciplinary design optimization. The paper presents the concept of Multi-objective Multidisciplinary design optimization techniques to support trade-off decisions between multiple conflicting design objectives and interdisciplinary design methodology, during the conceptual design of sport buildings. The proposed method is based on parametric modelling, performance simulation tools and algorithms for computational optimization, for which the paper tackles three specific aspects. First of all, due to the complexity of large sport buildings, the formulation of the optimization and the screening of the related design variables is crucial in order to obtain a meaningful design space, which helps reducing unnecessary computational burden. Secondly, assessing performance based on measurements and analyses is crucial and can be supported by performance simulations tools; however effectively integrating performance simulations tools in the early phase of the design requires new tools. In this light, a customized computational process for the rapid assessment of temperature and airflow patterns is presented. Thirdly, the process requires the combination of design optimization and design exploration, while searching for well-performing solutions. The importance of design exploration is emphasized also for sub-optimal solutions. In order to facilitate the design exploration, the combination of optimization algorithms, multi-variate analysis algorithms and options for exploring design solutions via an interactive dashboard connected to a database are presented. To exemplify the method, specific case studies are developed as collaboration between Delft university of Technology and South China university of Technology.Design InformaticsEnvironmental Technology and Desig

Simulating natural ventilation in large sports buildings: Prediction of temperature and airflow patterns in the early design stages

In large sport’s buildings, a big part of energy can be saved by providing natural instead of mechanical ventilation. However, additional challenges arise while controlling airflow and temperatures in different zones. These measureshighly depend on the shape, construction and ventilation openings, which are mostly decided in the early design stages. Computational optimization can support these early stages of design, but needs to be performed in efficient ways. In this respect, the project proposes rapid assessment of temperature and airflow patterns using customized Grasshopper components, which would be able to evaluate a given model using CONTAM and EnergyPlus software assimulation engine. The proposed method integrates these simulations within an environment, which is familiar to architects and is largely used for parameterization of design in its early stages. A case study (Jiangmen Sports Center, Jiangmen, China) is used to test the developed process for a large indoor sports hall