Implantation of Mouse Embryonic Stem Cell-Derived Cardiac Progenitor Cells Preserves Function of Infarcted Murine Hearts

Stem cell transplantation holds great promise for the treatment of myocardial infarction injury. We recently described the embryonic stem cell-derived cardiac progenitor cells (CPCs) capable of differentiating into cardiomyocytes, vascular endothelium, and smooth muscle. In this study, we hypothesized that transplanted CPCs will preserve function of the infarcted heart by participating in both muscle replacement and neovascularization. Differentiated CPCs formed functional electromechanical junctions with cardiomyocytes in vitro and conducted action potentials over cm-scale distances. When transplanted into infarcted mouse hearts, CPCs engrafted long-term in the infarct zone and surrounding myocardium without causing teratomas or arrhythmias. The grafted cells differentiated into cross-striated cardiomyocytes forming gap junctions with the host cells, while also contributing to neovascularization. Serial echocardiography and pressure-volume catheterization demonstrated attenuated ventricular dilatation and preserved left ventricular fractional shortening, systolic and diastolic function. Our results demonstrate that CPCs can engraft, differentiate, and preserve the functional output of the infarcted heart

Comparison of human and porcine insulin therapies in children with newly diagnosed diabetes mellitus.

A multicenter, longitudinal study of children below the age of 16 years with newly diagnosed Type 1 (insulin-dependent) diabetes treated either with porcine monocomponent insulin (n = 26) or semisynthetic human monocomponent insulin (n = 26) was performed during the first 24 months after onset of diabetes. The two groups were carefully matched for age, duration of disease symptoms, initial metabolic values, islet cell antibodies and HLA-DR antigens. During the 24-month observation period there was no significant difference between the two groups in respect to the clinical course, insulin dosage, HbA1 and residual B-cell activity. No child in either group had a real remission without necessitating insulin therapy. The prevalence of insulin antibodies increased slowly and was 62% in the group treated by human insulin and 52% in the porcine insulin-treated group after 24 months. The titres were generally low and there was no statistical difference between the two groups in respect to insulin antibody formation

Persistent microalbuminuria in adolescents with type I (insulin-dependent) diabetes mellitus is associated to early rather than late puberty. Results of a prospective longitudinal study

Microalbuminuria is generally accepted to be highly predictive of overt diabetic nephropathy which is the leading cause of end-stage renal failure and, consequently, of death in patients with type 1 (insulin-dependent) diabetes mellitus (IDDM). Its early identification and therapy are exceedingly important. We studied prospectively the occurrence of microalbuminuria (MA) in relation to puberty and its pubertal stages in 164 children and adolescent patients (83 girls and 81 boys) with IDDM. Analysing 100 healthy subjects, normal values for albumin excretion (range: 0-10.1 micrograms/min/1.73 m2) according to sex and the different pubertal stages were defined. No significant difference between the groups were noted and, therefore, 20 micrograms/min per 1.73 m2 (3 SD above the mean) was generally defined as cutoff for MA. Of the patients with IDDM studied, 20% (20 females and 12 males) developed persistent MA (22.1-448.2 micrograms/min/1.73 m2) during the study period of 8 years. The first manifestation of persistent MA was in 69% (13 females and 9 males) during stages of early and midpuberty; and in 28% (6 females and 3 males) at a late pubertal stage or at the end of puberty. The only child who developed MA before the onset of puberty (range: 23.5-157.4 micrograms/min/1.73 m2) was found to have dystopic kidney. Therefore, all patients with IDDM should be screened for MA regardless of diabetes duration, sex and level of diabetes control beginning at the very first stage of puberty and neither earlier nor after puberty as suggested by the American Diabetes Association

Information material for migrant populations in Switzerland: the example of physical activity promotion

In order to develop information material on physical activity and sports for migrant populations, the specific needs of these target groups with regard to motivation, barriers and accessibility were analysed. Based on the results, an information brochure was developed and its use was evaluated both by monitoring of incoming orders and by semi-structured interviews with professionals. The main motives and obstacles for physical activity of different migrant groups as well as other important topics to be covered were identified. Important insights could be gained regarding design and distribution. Brochures in eight different languages were published, ordered and distributed. Most of the conclusions from the needs assessment were confirmed in the evaluation and possibilities for further developments were identified

Integrative research efforts at the boundary of biodiversity and global change research

Global environmental change and biodiversity loss are closely linked through different feedback mechanisms. The University of Zurich Research Priority Programme on ‘Global Change and Biodiversity’ approach is to work with interdisciplinarity and transdisciplinarity to integrate mechanisms of interactions, feedback and scale and improve our understanding of the feedbacks between global change and biodiversity effects. Such work across research disciplines is not without its challenges. Here we share some of the questions that arose from our research approach over the last five years and how we addressed these challenges. First, our transdisciplinary approach allows combining different disciplines into a more holistic perspective towards integrative research, but demands collaborative work to establish common terminology, concepts, and metrics. Second, the research theme's common perspective (biodiversity is desirable, global change is not) may also induce a confirmation bias from preconceived ideas. Third, new challenges emerge from scaling mechanisms and feedbacks at different spatial and temporal scales. Fourth, we investigate how to relate biodiversity, global change, ecosystem services and functions using interdisciplinary approaches. Fifth, we identify gaps between existing experiments and data requirements, and propose the definition of new experimental setups by linking processes and performing experiments at typical experimental scales as well as at larger scales. We conclude by emphasising the necessity to integrate theory, experiments, modelling and simulation, high performance computing and big data to understand feedbacks between biodiversity loss and processes of global change