DNA Barcoding of Endophytic Bacteria from The Stems of Tissue Piper crocatum and Activity Anti-Bacterial Properties

Secondary metabolites from herbal are commonly used to treat disease due to infection from pathogenic bacteria, endophytic bacteria is shortcut to collect secondary metabolites more effective than extracting direct from the plant. This research aims to obtain endophytic bacterial isolates from the stems of the red betel plant which can be used as an inhibitor for the growth of pathogenic bacteria. The method of this research is exploratory descriptive experiment based research. The stages of this research consist of few techniques which are isolation of endophytic, antibacterial examination, morphological, physiological, and biochemical observation, DNA bacterial extraction, gel electrophoresis. To analyse DNA Barcoding using 16S rRNA gen, BLAST and MEGA 10. The results of the antibacterial activity of the isolates showed by clear zone formation against pathogenic bacteria such as E. coli, K. pneumoniae and S. aureus. Endophytic bacterial that successfully isolated are labelled (BSM1, BSM2, BSM3 and BSM4). Based on of the Gel electrophoresis shows that four endophytic bacterial isolates are positive by appearance of bands in 1324bp. Based on the results of the phylogenetic tree it can be concluded that BSM1 is closely related to Bacillus cereus JL with a genetic distance of 0.007, while the genetic distance of BSM2 was 0.012, BSM3 0.006 and BSM4 0.003, all three were closely related to Bacillus pumilus AUEC29

Estrogenic Effects of Tapak Dara (Catharantus roseus) Leaf Methanol Extract on The Estrus Cycle of Adult Female Mice (Mus musculus) Balb/C Strain

Tapak dara plant is a plant that contains several active compounds, such as phenolic acids, flavonoids and alkaloids. The tapak dara plant can act as a sedative, urinary laxative, stop bleeding, and neutralize toxins. In addition, tapak dara leaf extract contains ingredients that can act as antifertilization. This study aimed to determine the effect of methanol extract of tapak dara leaf on the estrus cycle of adult female mice (Mus musculus) Balb/C strain. This research is an experimental research by testing cause and effect. The hypothesis test used in this study was one-way ANOVA and BNT test. The significance value of giving tapak dara leaf methanol extract to female mice showed a significant value so that it can be said that the administration of tapak dara leaf methanol extract caused the estrus cycle of female mice to be longer. The significance value of estrogenic activity in mice shows a significant value of 0.047, so it can be said that estrogen activity also affects the length of the estrus cycle. The results of hypothesis testing using the one-way ANOVA test were significant, so it can be concluded that the methanol extract of tapak dara leaves can cause the estrus cycle of female mice to be longer

APLIKASI SISTEM BAGANG APUNG SEDERHANA DENGAN TEKNOLOGI KRAMBA JARING APUNG UNTUK BUDIDAYA NELAYAN KECIL DI DESA KETAPANG RAYA LOMBOK TIMUR

Masalah utama nelayan kecil di lokasi pengabdian adalah hasil tangkapan ikan yang terus menurun. Implikasinya, mereka tidak dapat memenuhi kebutuhan dasar dari hasil melaut. Oleh karena itu dibutuhkan program pemberdayaan yang dapat menjadi solusi matapencaharian yang berkelanjutan. Program pemberdayaan yang relevan untuk nelayan kecil di lokasi pengabdianà adalah budidaya laut. Sistem budidaya yang dikembangkan adalah sistem integrasi bagang apung sederhana dengan teknologi keramba jaring apung (KJA). Tujuan dari programà ini adalah kelompok nelayan kecil dapat melakukan budidaya sebagai suplemen matapencaharian berkelanjutan melalui penerapan teknologi KJA yang terintegrasi dengan bagang apung sederhana. Target khusus dari program ini adalah transfer teknologi budidaya pada nelayan kecil untuk keberlanjutan ekonomi dan lingkungan. Pendekatan yang digunakan adalah pendekatan kooperatif melalui pelatihan model pembelajaran orang dewasa (andragogi) dan pembuatan contoh model penerapan integrasi bagang apung sederhana dengan teknologi KJA. Hasil dari program pengabdian pada masyarakat, bahwa sistem produksi dengan teknologi bagang apung dalam satu unit produksi cukup efektif dan efisien untuk keberlanjutan budidaya ramah lingkungan nelayan kecil pada lokasi pengabdian. Selain itu, nelayan kecil memiliki optimisme terhadap matapencaharian tambahan dari usaha budidaya dalam memenuhi kebutuhan keluarga. Oleh karena itu, pengembangan teknologi budidaya dapat dipertimbangakan dalam kebijakan pemberdayaan masyarakat pesisir

Hubungan Pemahaman Nilai-Nilai Karakter Peduli Lingkungan Dengan Hasil Belajar Biologi

Kepedulian siswa terhadap lingkungan masih rendah, hal ini dibuktikan dengan tampak sampah disekitar. Penelitian ini bertujuan untuk mengetahui hubungan pemahaman nilai-nilai karakter peduli lingkungan dengan  hasil belajar biologi peserta didik SMAN 2 Selong. Jenis penelitian adalah penelitian korelasional dengan pendekatan kuantitatif. Populasi penelitian adalah seluruh peserta didik kelas X dan XI IPA SMAN 2 Selong berjumlah 547 peserta didik. Jumlah sampel ditentukan berdasarkan Tabel Cohen Manion dan Morrison,  jumlah sampel terpilih sebanyak 270 peserta didik. Teknik pengumpulan data menggunakan  angket dan dan studi dokumentasi. Analisis data dengan teknik uji korelasi Rank Spearman. Hasil uji korelasi menunjukkan nilai signifikansi korelasi sebesar 0,000 lebih kecil dari 0,05 sehingga dapat nyatakan bahwa ada hubungan positif dan signifikan antara pemahaman nilai-nilai karakter peduli lingkungan dengan hasil belajar Biologi peserta didik SMAN 2 Selong Tahun Pelajaran 2021/2022

Isolation of endophytic bacteria from cashew root and its ability as phosphate solubilizing and IAA-producing bacteria

This study aimed to isolate endophytic bacteria from cashew plant roots, and to determine their ability to dissolve phosphate and produce IAA. This research was conducted at the Microbiology Laboratory of the Faculty of Medicine, University of Mataram. This research was conducted by isolating the endophytic bacteria of cashew nut root and then testing the phosphate solubilizing ablity and testing the potential for IAA-producing bacteria. Based on the results of the study, there were 7 isolates of endophytic bacteria, all of which were able to solubilize inorganc phosphate with a low phosphate solubility index, ranging from 1 to 1.7. Of the 7 isolates, there were 2 isolates that were able to produce IAA, namely AJM 7 and AJM8 isolates with IAA concentrations ranging from 16 to 18 ppm. Based on the results above, it can be concluded that the endophytic bacterial isolates from the roots of the cashew plant have the potential to be a candidates for biofertilizer formulation

Isolation of endophytic bacteria from the roots of Gliricidia sepium and their ability as IAA-producing bacteria and phosphate solubilizers

This study aimed to isolate endophytic bacteria from the roots of the gamal plant and to test its ability to solubilize anorganic phosphate dan to produce IAA (indol acetic acid). The steps in this study were the isolation of endophytic bacteria from the roots of the gamal plant taken from Woja District, Dompu Regency, the IAA test, and the phosphate solubility test using the colorimetric method. And then carried out the characterization of cells, colonies and biochemical properties of isolates. In this study, 6 isolates of endophytic bacteria from the roots of gamal plants were obtained which can dissolve phosphate, of which two isolates, namely ATG1 and ATG4 with phosphate solubility indices ranging from 3 and 4. These two isolates were also capable of producing IAA, with concentrations 16.48 and 17.72 ppm, respectively. Most of the isolates were Gram positive bacteria and were in the form of bacilli. The endophytic isolates obtained above are isolates that have the potential to be candidates for components of the biofertilizer formulation consortium in the future

Pengaruh Konsentrasi Ragi dan Lama Fermentasi terhadap Kadar Air, Glukosadan Organoleptik pada Tape Singkong

Tujuan dari penelitian ini adalah mengetahuipengaruh (1) konsentrasi ragi, (2) lama fermentasi serta (3) interaksi antara konsentrasi ragi dan lama fermentasi terhadap kadar air, glukosa dan organoleptik pada tape singkong. Penelitian dilakukan di Laboratorium Kimia Analitik Universitas Mataram dan Fakultas Keguruan dan Ilmu Pendidikan Universitas Mataram. Bahan yang digunakan dalam penelitian adalah tape singkong. Penelitian dilaksanakan secara eksperimental berdasarkan RAL pola faktorial. Faktor I konsentrasi ragi (0,5; 1 dan 1,5%) dan faktor II lama fermentasi (1, 2 dan 3 hari) dengan 3 kali ulangan. Parameter penelitian meliputi kadar air, glukosa dan organoleptik. Hasil penelitian menunjukkan bahwa(1)Konsentrasi ragiberpengaruh terhadap kadar air(p ≤ 0,05) dan organoleptik (p0,05)pada kadar glukosatape singkong.(2) Lama fermentasiberpengaruh terhadap kadar air (p0,05) dan organoleptik (p>0,05) tape singkong

Pengaruh Konsentrasi Ragi Dan Lama Fermentasi Terhadap Kadar Air, Glukosadan Organoleptik Pada Tape Singkong

Abstrak Tujuan dari penelitian ini adalah mengetahuipengaruh (1) konsentrasi ragi, (2) lama fermentasi serta (3) interaksi antara konsentrasi ragi dan lama fermentasi terhadap kadar air, glukosa dan organoleptik pada tape singkong. Penelitian dilakukan di Laboratorium Kimia Analitik Universitas Mataram dan Fakultas Keguruan dan Ilmu Pendidikan Universitas Mataram. Bahan yang digunakan dalam penelitian adalah tape singkong. Penelitian dilaksanakan secara eksperimental berdasarkan RAL pola faktorial. Faktor I konsentrasi ragi (0,5; 1 dan 1,5%) dan faktor II lama fermentasi (1, 2 dan 3 hari) dengan 3 kali ulangan. Parameter penelitian meliputi kadar air, glukosa dan organoleptik. Hasil penelitian menunjukkan bahwa(1)Konsentrasi ragiberpengaruh terhadap kadar air(p ≤ 0,05) dan organoleptik (p0,05)pada kadar glukosatape singkong.(2) Lama fermentasiberpengaruh terhadap kadar air (p0,05) dan organoleptik (p>0,05) tape singkong. Kata kunci: konsentrasi ragi, lama fermentasi, kadar air, kadar glukosa, organoleptik,  AbstractThe purpose of this research arethe influence of (1) concentration of yeast, (2) long fermentingas well as (3) interaction between concentration of yeast and long fermentingagainst the levels of water, glucose and organoleptik on fermented cassava. Research was conducted in Laboratory ChemistryAnalytic of Mataram University and Faculty of Education of Mataram University. Material used in this research are fermented cassava. Thisresearch is experimentresearch with Random Complete Design factorial pattern. Factor I concentration of yeast (0,5; 1 and 1,5%) factor II long fermenting(1, 2 and 3day) with 3 repetition. Parameters research covering the levels of water, glucose and organoleptik. The results of this research are (1) Concentration of yeastinfluential on the levels of water (p ≤ 0,05) and organoleptik (p0,05) on glucose level of fermented cassava. (2) Long fermentinginfluential on the levels of water (p0,05)  and organoleptik (p >0,05)  of fermented cassava. Keyword: yeast concentration, fermentations time, levels of  water, levels of glucose, organoleptik 

Isolation of Endophytic Fungi from Vitex trifolia L and Antagonism Test against Sclerotium rolfsii and pathogenic bacteria

Plant damage by pathogenic fungi is often found in plants, one of which is caused by Sclerotium rolfsii. Biological control strategy offers a promising alternative for managing disease in plants because they are environmental friendly compared to pesticides application. One of the biological control offered is by using endophytic fungi isolated from Vitex trivolia L. The aim of the study was to isolate, to identify macroscopic and microscopic endophytic fungi from Vitex trifolia L and to test their antagonism potency against the pathogenic fungus Sclerotium rolfsii in vitro. The isolation obtained 7 endophytic fungi isolates identified based on their genus characteristics, nsmely Periconia sp, Aspergillussp, Dendrophoma  sp, Geotrichum  sp, Ampulliferina  sp, Chalara  sp, dan Bispora sp and 2 isolates have not been identified. The Antibacterial test of the fungi isolate on the 4 tested bacteria showed that of all the fungi isolate have low activity. The antagonism test using the direct opposition method with the PIRG formula, showed that the 3 isolates had high percentage of growth inhibition, in which ALJ1, BLJ5, and ALJ3 isolate has  85%, 90%, and 100% respectively. This potency could be used as biological agents on the pathogenic fungus Sclerotium rolfsii

The Effect of Burned Rice Husks and NPK Fertilizer Application on The Growth of Bok Choy (Brassica Rapa L. Subsp. Chinensis)

Fertilizer is very important factor in achieving high productivity, especially in providing essential nutrients. The application of inorganic fertilizers without adding organic fertilizers potentially reduces soil fertility and damages soil structure. This study aimed to determine the effect of burned rice husks, NPK fertilizer application, on the growth of Chinensis bok choy (Brassica rapa L. Subsp. Chinensis). The study employed a completely randomized design (CRD) consisting of 2 factors, with 5 treatment levels. The burned rice husks treatments comprised (0 gr, 90 gr, 110 gr, 130 gr, and 150 gr), while the NPK fertilizer treatments comprised (0 gr, 0.4 gr, 0.6 gr, 0.8 gr, 1.0 gr / 100 ml water). Data were analyzed using an ANOVA test. The results showed that the NPK fertilizer factor significantly influenced all parameters, including plant height, leaf number, wet weight, and dry weight. The burned rice husks factor and the interaction between burned rice husks and NPK fertilizer had no significant effect on all parameters, namely plant height, leaf number, wet weight, and dry weight of the Chinensis bok choy. The optimal treatment for the interaction of burned rice husks and NPK fertilizer "Mutiara 16" was with the N4S4 treatment (combination of NPK Mutiara 1.0 gr/100 ml water and burned rice husks 150 gr) for plant height, leaf number, wet weight, and dry weight parameters, while the optimal treatment for chlorophyll concentration content was with the N3S2 treatment (combination of NPK Mutiara 0.8 gr/100 ml water and burned rice husks 110 gr)