Ape1 regulates hematopoietic differentiation of embryonic stem cells through its redox functional domain

Ape1 is a molecule with dual functions in DNA repair and redox regulation of transcription factors. In Ape1-deficient mice, embryos do not survive beyond embryonic day 9, indicating that this molecule is required for normal embryo development. Currently, direct evidence of the role of Ape1 in regulating hematopoiesis is lacking. We used the embryonic stem (ES) cell differentiation system and an siRNA approach to knockdown Ape1 gene expression to test the role of Ape1 in hematopoiesis. Hemangioblast development from ES cells was reduced 2- to 3-fold when Ape1 gene expression was knocked down by Ape1-specific siRNA, as was primitive and definitive hematopoiesis. Impaired hematopoiesis was not associated with increased apoptosis in siRNA-treated cells. To begin to explore the mechanism whereby Ape1 regulates hematopoiesis, we found that inhibition of the redox activity of Ape1 with E3330, a specific Ape1 redox inhibitor, but not Ape1 DNA repair activity, which was blocked using the small molecule methoxyamine, affected cytokine-mediated hemangioblast development in vitro. In summary, these data indicate Ape1 is required in normal embryonic hematopoiesis and that the redox function, but not the repair endonuclease activity, of Ape1 is critical in normal embryonic hematopoietic development

Extending the Propagation Distance of a Silver Nanowire Plasmonic Waveguide with a Dielectric Multilayer Substrate

Chemical synthesized silver nanowires have been proved to be the efficient architecture for Plasmonic waveguides, but the high propagation loss prevents their widely applications. Here, we demonstrate that the propagation distance of the plasmons along the Ag NW can be extended if the Ag NW was placed on a dielectric multilayer substrate containing a photonic band gap, but not placed on a commonly used glass substrate. The propagation distance at 630 nm wavelength can reach 16 um even that the Ag NW is as thin as 90 nm in diameter. Experimental and simulation results further show that the polarization of this propagating plasmon mode was nearly parallel to the surface of the dielectric multilayer, so it was excited by a transverse-electric polarized Bloch surface wave propagating along a polymer nanowire with diameter at only about 170 nm on the same dielectric multilayer. Numerical simulations were also carried out and consistent with the experiment results. Our work provides a platform to extend the propagation distance of plasmonic waveguide and also for the integration between photonic and plasmonic waveguides on the nanometre scale.Comment: 5 pages, 4 figure

Non-reciprocal cavity polariton

Breaking the time-reversal symmetry of light is of great importance for fundamental physics, and also have attracted increasing interests in the study of non-reciprocal photonics for applications. The optical non-reciprocity has been realized by engineering the susceptibility of dielectric, through either magneto-optics effect with bias magnetic field or directional coherent nonlinear optical effects stimulated by external drives. Here, we experimentally demonstrate an inherently non-reciprocal quasiparticle, i.e. the cavity polariton, in a strongly coupled cavity quantum electrodynamics system. Through carefully manipulating the internal quantum state of atoms to break the time-reversal symmetry, the polariton shows non-reciprocal photon emission without bias field. Such non-reciprocal polariton state leads to optical isolation exceeds 30dB on single-quanta level ($\sim0.1$ photon), and also produces non-reciprocal non-classical statistics with coherent probe lights, manifesting the quantum nature of the non-reciprocal polaritons. Such new quasiparticle demonstrated in this work holds great potential for exploring the quantum non-reciprocity in photonics and quantum network applications, and also new topological phases in many-body physics.Comment: 6 pages, 4 figure

Asymmetric α-amination of 3-substituted oxindoles using chiral bifunctional phosphine catalysts

Abstract A highly enantioselective α-amination of 3-substituted oxindoles with azodicarboxylates catalyzed by amino acids-derived chiral phosphine catalysts is reported. The corresponding products containing a tetrasubstituted carbon center attached to a nitrogen atom at the C-3 position of the oxindole were obtained in high yields and with up to 98% ee. 72

Reconstruction Method for Nonconcyclic Dual-Source Circular Cone-Beam CT with a Large Field of View

In industrial computed tomography (CT), it is often required to inspect large objects whose size is beyond a reconstructed field of view (FOV). Some multiscan modes have been developed to acquire the complete CT projection data for a larger object using small panel detectors. In this paper, we give a non-concyclic dual-source circular cone-beam scanning geometry based on the idea of multiscan modes and propose a backprojection-filtration-based (BPF) reconstruction algorithm without data rebinning. Since the FOV calculated according to this nonconcyclic dual-source circular CT scanning geometry is larger than cardiac dual-source CT scanning geometry, our method can reconstruct larger horizontal slices (i.e., the slices perpendicular to rotation axis) than cardiac dual-source CT. The quality of CT images is expected to be superior to those obtained using larger panel detectors. The simulation results have indicated that CT images obtained by the proposed method are satisfying