A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids-1

<p><b>Copyright information:</b></p><p>Taken from "A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids"</p><p>http://www.biomedcentral.com/1471-2199/9/54</p><p>BMC Molecular Biology 2008;9():54-54.</p><p>Published online 4 Jun 2008</p><p>PMCID:PMC2443376.</p><p></p

A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids-7

<p><b>Copyright information:</b></p><p>Taken from "A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids"</p><p>http://www.biomedcentral.com/1471-2199/9/54</p><p>BMC Molecular Biology 2008;9():54-54.</p><p>Published online 4 Jun 2008</p><p>PMCID:PMC2443376.</p><p></p

A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids-3

transgenic gene, five different GM contents (10.0%, 5.0%, 1.0%, 0.5% and 0.1%) of soybean DNA that contained 10, 5, 1, 0.5 and 0.1 ng GM soybean DNA per reaction, respectively, were plotted as the initial RRS DNA concentration versus Ct standard curve (R= 0.9964). (C) Standard curve, plotting log [GMOs concentration] versus change in threshold of detection (Ct), R= 0.9964.<p><b>Copyright information:</b></p><p>Taken from "A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids"</p><p>http://www.biomedcentral.com/1471-2199/9/54</p><p>BMC Molecular Biology 2008;9():54-54.</p><p>Published online 4 Jun 2008</p><p>PMCID:PMC2443376.</p><p></p

A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids-0

E second cycle of amplification. (D) The third cycle of amplification and fluorescent signal generation; (E), (Fa) and (Fb), The fourth cycle of amplification and fluorescent signal generation.<p><b>Copyright information:</b></p><p>Taken from "A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids"</p><p>http://www.biomedcentral.com/1471-2199/9/54</p><p>BMC Molecular Biology 2008;9():54-54.</p><p>Published online 4 Jun 2008</p><p>PMCID:PMC2443376.</p><p></p

A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids-2

Nsgenic soybean ranging from 0.01 to 100 ng with CaMV35s promoter real-time PCR system; b: Initial DNA concentration versus Ct standard curve; (B) The standard curve of NOS terminator quantification. a: Amplification plot generated by five dilutions (10-fold) of transgenic soybean ranging from 0.01 to 100 ng with NOS terminator real-time PCR system; b: Initial DNA concentration versus Ct standard curve.<p><b>Copyright information:</b></p><p>Taken from "A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids"</p><p>http://www.biomedcentral.com/1471-2199/9/54</p><p>BMC Molecular Biology 2008;9():54-54.</p><p>Published online 4 Jun 2008</p><p>PMCID:PMC2443376.</p><p></p

A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids-6

E second cycle of amplification. (D) The third cycle of amplification and fluorescent signal generation; (E), (Fa) and (Fb), The fourth cycle of amplification and fluorescent signal generation.<p><b>Copyright information:</b></p><p>Taken from "A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids"</p><p>http://www.biomedcentral.com/1471-2199/9/54</p><p>BMC Molecular Biology 2008;9():54-54.</p><p>Published online 4 Jun 2008</p><p>PMCID:PMC2443376.</p><p></p