E second cycle of amplification. (D) The third cycle of amplification and fluorescent signal generation; (E), (Fa) and (Fb), The fourth cycle of amplification and fluorescent signal generation.<p><b>Copyright information:</b></p><p>Taken from "A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids"</p><p>http://www.biomedcentral.com/1471-2199/9/54</p><p>BMC Molecular Biology 2008;9():54-54.</p><p>Published online 4 Jun 2008</p><p>PMCID:PMC2443376.</p><p></p