6 research outputs found
Meat quality, fatty acid profile and genomic insight of Busha cattle in extensive production systems in Serbia
Identification of SNPs and effective genes associated with meat quality traits and fatty acid
(FA) profiles can help in the selection of animals with the best potential. In this study, we
characterized the meat quality parameters in young Busha bulls for the first time and further
performed GWAS analysis for meat quality traits. The fatty acid profile showed that
saturated fatty acids were the most abundant in meat and that oleic fatty acid, which is a
monounsaturated fatty acid, had the highest content. The correlation analysis showed that
the protein content had a negative correlation (p < 0.05) with the pH value and a positive
correlation (p < 0.05) with the content of polyunsaturated fatty acids. GWAS analysis was
performed with the BovineSNP50 v3 DNA Analysis BeadChip using the Illumina IscanTM
platform. The data were analyzed with PLINK 1.9. Thirteen SNPs were identified with
genome wide significant association with muscle fiber diameter (MFD) and one with meat
pH. Several candidate genes for MFD have been identified, including APOD, NTMT2 and
ZBTB37. Candidate
SNPs with near suggestive signifficant association with multiple fatty
acids level were identified: ARS-BFGL-NGS-118200, Hapmap48202-BTA-118947, BTA-
112619-no-rs. ANGPTL3 could be a potential new candidate gene influencing the FA profile.
This study provided valuable insights into the genetics of Busha meat quality traits.
The findings from this study could be used for the sustainable exploitation of this autochthonous
cattle breed
Effects of NADPH oxidase blockade and hyperbaric oxygen preconditioning on 4- HNE, NGAL, and HO-1 tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rats : [abstract]
The interpretation of the reasons for encounter âcoughâ and âsadnessâ in four international family medicine populations
Background This is a study of the relationships between common reasons for encounter and common diagnoses (episode titles) within episodes of care in family practice populations in four countries.Method Participating family doctors recorded details of all their patient contacts in an EoC structure using the International Classification of Primary Care, including RfEs presented by the patient, and the FDsâ diagnostic labels. The relationships between RfEs and episode titles were studied using Bayesian methods.Results The RfE âcoughâ is a strong, reliable predictor for the diagnoses âcoughâ, âacute bronchitisâ, âURTIâ and âacute laryngitis/tracheitisâ and a less strong, but reliable predictor for âsinusitisâ, âpneumoniaâ, âinfluenzaâ, âasthmaâ, âother viral diseasesâ, âwhooping coughâ, âchronic bronchitisâ, âwheezingâ and âphlegmâ. The absence of cough is a weak but reliable predictor to exclude a diagnosis of âcoughâ, âacute bronchitisâ and âtracheitisâ. Its presence allows strong, reliable exclusion of the diagnoses âgastroenteritisâ, âno diseaseâ and âhealth promotion/preventionâ, and less strong exclusion of âadverse effects of medicationâ. The RfE âsadnessâ is a strong, reliable predictor for the diagnoses âfeeling sad/depressedâ and âdepressive disorderâ. It is a less strong, but reliable predictor of a diagnosis of âacute stress reactionâ. The absence of sadness is a weak but reliable predictor to exclude the symptom diagnosis âfeeling sad/depressedâ. Its presence does not support the exclusion of any diagnosis.Conclusions We describe clinically and statistically significant diagnostic associations observed between the RfEs âcoughâ and âsadnessâ, presenting as a new problem in family practice, and all the episode titles in ICPC
Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of IschemiaâReperfusion-Induced Acute Kidney Injury
Ischemiaâreperfusion injury (IRI) is a frequent cause of AKI, resulting in vasoconstriction, cellular dysfunction, inflammation and the induction of oxidative stress. DNA damage, including physical DNA strand breaks, is also a potential consequence of renal IRI. The histone H2A variants, primary H2AX and H2AZ participate in DNA damage response pathways to promote genome stability. The aim of this study was to evaluate the immunohistochemical pattern of histone H2A variantsâ (H2AX, ÎłH2AX(S139), H2AXY142ph and H2AZ) expression in an experimental model of ischemiaâreperfusion-induced acute kidney injury in spontaneously hypertensive rats. Comparing the immunohistochemical nuclear expression of ÎłH2AX(S139) and H2AXY142ph in AKI, we observed that there is an inverse ratio of these two histone H2AX variants. If we follow different regions from the subcapsular structures to the medulla, there is an increasing extent gradient in the nuclear expression of H2AXY142ph, accompanied by a decreasing nuclear expression of ÎłH2AX. In addition, we observed that different structures dominated when ÎłH2AX and H2AXY142ph expression levels were compared. ÎłH2AX was expressed only in the proximal tubule, with the exception of when they were dilated. In the medulla, H2AXY142ph is predominantly expressed in the loop of Henle and the collecting ducts. Our results show moderate sporadic nuclear H2AZ expression mainly in the cells of the distal tubules and the collecting ducts that were surrounded by dilated tubules with PAS (periodic acidâSchiff stain)-positive casts. These findings may indicate the degree of DNA damage, followed by postischemic AKI, with potential clinical and prognostic implications regarding this condition
An international comparative family medicine study of the Transition Project data from the Netherlands, Malta and Serbia. Is family medicine an international discipline? Comparing incidence and prevalence rates of reasons for encounter and diagnostic titles of episodes of care across populations
Item does not contain fulltextINTRODUCTION: This is a study of the epidemiology of family medicine (FM) in three practice populations from the Netherlands, Malta and Serbia. Incidence and prevalence rates, especially of reasons for encounter (RfEs) and episode labels, are compared. METHODOLOGY: Participating family doctors (FDs) recorded details of all their patient contacts in an episode of care (EoC) structure using electronic patient records based on the International Classification of Primary Care (ICPC), collecting data on all elements of the doctor-patient encounter. RfEs presented by the patient, all FD interventions and the diagnostic labels (EoCs labels) recorded for each encounter were classified with ICPC (ICPC-2-E in Malta and Serbia and ICPC-1 in the Netherlands). RESULTS: The content of family practice in the three population databases, incidence and prevalence rates of the common top 20 RfEs and EoCs in the three databases are given. CONCLUSIONS: Data that are collected with an episode-based model define incidence and prevalence rates much more precisely. Incidence and prevalence rates reflect the content of the doctor-patient encounter in FM but only from a superficial perspective. However, we found evidence of an international FM core content and a local FM content reflected by important similarities in such distributions. FM is a complex discipline, and the reduction of the content of a consultation into one or more medical diagnoses, ignoring the patient's RfE, is a coarse reduction, which lacks power to fully characterize a population's health care needs. In fact, RfE distributions seem to be more consistent between populations than distributions of EoCs are, in many respects
An international comparative family medicine study of the Transition Project data from the Netherlands, Malta, Japan and Serbia. An analysis of diagnostic odds ratios aggregated across age bands, years of observation and individual practices
Item does not contain fulltextINTRODUCTION: This is a study of the process of diagnosis in family medicine (FM) in four practice populations from the Netherlands, Malta, Serbia and Japan. Diagnostic odds ratios (ORs) for common reasons for encounter (RfEs) and episode titles are used to study the process of diagnosis in international FM and to test the assumption that data can be aggregated across different age bands, practices and years of observation. METHODOLOGY: Participating family doctors (FDs) recorded details of all their patient contacts in an episode of care (EoC) structure using the International Classification of Primary Care (ICPC). RfEs presented by the patient and the diagnostic labels (EoC titles) recorded for each encounter were classified with ICPC. The relationships between RfEs and episode titles were expressed as ORs using Bayesian probability analysis to calculate the posterior (post-test) odds of an episode title given an RfE, at the start of a new EoC. RESULTS: The distributions of diagnostic ORs from the four population databases are tabled across age groups, years of observation and practices. CONCLUSIONS: There is a lot of congruence in diagnostic process and concepts between populations, across age groups, years of observation and FD practices, despite differences in the strength of such diagnostic associations. There is particularly little variability of diagnostic ORs across years of observation and between individual FD practices. Given our findings, it makes sense to aggregate diagnostic data from different FD practices and years of observation. Our findings support the existence of common core diagnostic concepts in international FM